پیش‌بینی اپی توپ‌های سلول‌هایB و Tآنتی‌ژن Omp25 از باکتری بروسلا ملی تنسیس به منظور طراحی واکسن نوترکیب گوسفندی

Subject Areas : Camel

س. یوسفی ¹ , م. طهمورث‌پور ² , م.ه. سخاوتی ³

1 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
2 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran
3 - Department of Animal Science, Ferdowsi University of Mashhad, Mashhad, Iran

Received: 2014-03-25 Accepted : 2014-06-15 Published : 2015-09-01

Keywords: بروسلا, پیش بینی اپی‌ توپ, Omp25‌,

Abstract :

بروسلوز یکی از رایج ترین بیماری‌های دامی است که توسط باکتری گرم منفی بروسلا ایجاد می­شود. با توجه به ضرر­های جدی اقتصادی و درمانی این بیماری که برای دام و انسان همواره به ارمغان دارد تلاش­های بسیاری جهت جلوگیری و درمان این بیماری توسط واکسن­های نوترکیب بر پایه آنتی‌ژن­های غشای پروتئینی خارجی صورت می­گیرد. بدین منظور هدف از مطالعه حاضر بررسی خصوصیات بیوانفورماتیکی آنتی ژن Omp25 به عنوان یکی از آنتی‌ژن‌های غالب غشای پروتئینی باکتری بروسلا بوده است. در این پژوهش از نرم افزار­های بیوانفورماتیکی مختلفی برای پیش‌بینی اپی توپ­های B وT، ساختار دوم و سوم پروتئین، خصوصیات ایمنی‌زایی و ویژگی­های هضم پروتئین استفاده گردید. پیش از استفاده از نرم افزار‌ها میزان دقت آنها توسط داده‌های تجربی اعتبار سنجی گردید. نتایج آنالیز بیوانفورماتیکی نشان داد که پنج اپی توپ برای سلول‌های B در موقعیت‌های 44-26، 79-59، 112-88، 166-146، 202-175 و پنج اپی توپ برای سلول‌های T در مکان‌های 10-1، 22-14، 132-122، 162-154 و 213-206 وجود دارد. تمامی اپی توپ‌های شناسایی شده به جز اپی توپ‌های 10-1 و 22-14 دارای توانایی ایمنی‌زایی بودند. نهایتاً اپی توپ ناحیه 162-154 به عنوان یک اپی توپ مشترک بین سلول‌های B و T جهت طراحی واکسن نوترکیب پیش‌بینی گردید.

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