Effects of Glutamine Supplementation on Growth Performance and Antioxidant Status in Broilers with Pulmonary Hypertension Syndrome (PHS)
محورهای موضوعی : CamelM. Fathi 1 , T. Tanha 2 , M. Daneshyar 3
1 - Department of Animal Science, Faculty ofAgriculture, Payame Noor University, Tehran, Iran
2 - Department of Animal Science, Faculty ofAgriculture, Payame Noor University, Tehran, Iran
3 - Department of Animal Science, Uremia University, Uremia, Iran
کلید واژه: ascites, Lipid peroxidation, Antioxidant enzymes, glutamine,
چکیده مقاله :
The present study examined the antioxidant defense of glutamine on plasma and liver antioxidant status in broilers under PHS with respect to changes in the levels of lipid peroxidation marker malondialdehyde (MDA), total antioxidant status (TAS) and the activities of anti peroxidative enzymes [superoxide dismutase (SOD) and glutathione dependent antioxidant enzymes glutathione peroxidase (GPX)] in the plasma and liver tissue. Sampling of blood and liver tissue were determined at d 21 and 42. At the end of the experiment, 2 chicks from each replicate were randomly selected and slaughtered. The heart was removed; the right ventricle was dissected away from the left ventricle and septum, then the ratio of right ventricle weight to total ventricle weight (RV/TV) calculated too. Average BW gain and average feed intake were measured weekly from d 15 and average feed conversion ratio was calculated and reported weekly. The results showed that glutamine supplementation improve average feed conversion ratio (P<0.05). Moreover, in bird with PHS, supplementation of glutamine significantly (P<0.05) reduced the levels of MDA in plasma and liver tissue. A parallel increase glutathione peroxidase (GPX) activates in plasma and liver tissue. But SOD activity in plasma and liver tissue did not affected by glutamine supplementation. Also, glutamine significantly (P<0.05) reduced right ventricle weight to total ventricle weight (RV/TV) and mortality.
مطالعه حاضر با تأکید بر توان دفاعی گلوتامین بر وضعیت آنتی‌اکسیدانی پلاسما و کبد، تغییرات سطوح پراکسیداسیون چربی(MDA)، وضعیت آنتی‌اکسیدانی کل پلاسما (TAS) و فعالیت آنزیم‌های آنتی‌اکسیدان (SOD) و آنزیم وابسته به گلوتاتیون (GPX) در پلاسما و کبد جوجه‌های گوشتی در گیر با سندرم آسیت، انجام شد. نمونه برداری از خون و بافت کبد در روزهای 21 و 42 انجام شد. در پایان آزمایش، از هر قفس، 2 جوجه، به طور تصادفی انتخاب شده و بعد از کشتار، قبل آنها برداشته شد و بطن راست و بطن چپ از ناحیه سپتوم، جدا گشته و نسبت بطن راست به کل بطن‌ها (RV/TV)، نیز محاسبه گشت. میانگین خوراک مصرفی، افزایش وزن حاصله و ضریب تبدیل غذایی نیز به طور هفتگی، از روز 15، اندازه‌گیری شدند. نتایج نشان داد که، مکمل‌سازی گلوتامین سبب بهبود ضریب تبدیل غذایی شد (05/0P<). علاوه بر این، مکمل‌سازی گلوتامین در پرندگان آسیتی، سبب کاهش معنی‌دار MDA در پلاسما و بافت کبد گشت. همچنین، گلوتامین، سبب افزایش هم زمان فعالیت آنزیم GPX در پلاسما و بافت کبد شد. فعالیت آنزیم SOD در پلاسما و کبد، به طور معنی‌داری تحت تأثیر گلوتامین قرار نگرفت (05/0P>). علاوه بر این، مکمل‌سازی گلوتامین، سبب کاهش معنی‌دار تلفات آسیتی و RV / TV شد.
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