The Effect of in ovo Supplementation of Nano Zinc Oxide Particles on Hatchability and Post-Hatch Immune System of Broiler Chicken

محورهای موضوعی : Camel

آ. بیریا ¹ (Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran)
ب. نویدشاد ² (Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran)
ف. میرزایی آقجه قشلاق ³ (Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran)
س. نیک بین ⁴ (Department of Animal Science, University of Mohaghegh Ardabili, Ardabil, Iran)

تاریخ دریافت : 1397/10/09 تاریخ پذیرش : 1398/01/11 تاریخ انتشار : 1399/06/11

کلید واژه: performance, Broiler, antibody titer, immune system, hatchability, <i>in ovo</i> injection, nanoparti-cles of zinc,

چکیده مقاله :

The aim of this study was to evaluate the effects of in ovo injection of zinc oxide nanoparticles on the hatchability, production performance and immune responses of broilers. A total of 192 fertile eggs obtained from a Ross 308 broiler breeder flock were used. The eggs were divided into 4 experimental groups including a positive control group with normal saline injection, and injection of 50, 75 and 100 ppm nano-ZnO on the first day of incubation period in the air cell and were placed in the incubator. The hatched chicks were allocated to a completely randomized design with 4 treatments and 4 replicates for a 42-day experiment. In ovo injection of nano-ZnO increased hatchability of eggs compared to the control group (p &lt; 0.05). The daily feed intake during the starter phase significantly increased in the birds in 50 and 75 ppm nano-ZnO groups (p &lt; 0.05). At the grower phase in rearing period the growth rate and feed intake of all the nano-ZnO injected groups were higher than the control group (p &lt; 0.05). There was no significant difference in the feed conversion ratio between different treatment groups (P&gt;0.05). The results of antibody titer against the influenza virus at 10 days of age did not differ between the experimental treatments (P&gt;0.05). The heterophil, lymphocytes, monocytes and hematocrit number in the blood of broiler chickens at 10 days of age did not show any significant difference (P&gt;0.05). The results of the present study suggest that in ovo injection of nano ZnO particles had positive effects on early embryo mortality rate, total white blood cells, however the performance traits of the hatched chicks were not significantly affected through in ovo injection of nano ZnO particles.

چکیده انگلیسی:

هدف از این مطالعه بررسی اثرات تزریق درون تخم مرغی نانوذرات اکسید روی بر قابلیت جوجه درآوری، عملکرد تولیدی و پاسخ&shy;های ایمنی جوجه&shy;های گوشتی بود. از 192 تخم بارور تهیه شده از گله مرغ مادر گوشتی راس 308 استفاده شد. تخم&shy;ها به چهار گروه آزمایشی شامل یک گروه کنترل مثبت با تزریق سرم نمکی نرمال و تزریق 50، 75 و&nbsp; 100 ppm نانوذرات اکسید روی در روز اول دوره جوجه&shy;کشی در سلول هوایی تقسیم شده و در دستگاه جوجه&shy;کشی قرار گرفتند. جوجه&shy;های هچ شده در قالب یک طرح کاملاً تصادفی با چهار تیمار و چهار تکرار برای یک آزمایش 42 روزه مورد استفاده قرار گرفتند. تزریق درون تخم مرغی نانو ZnO باعث افزایش قابلیت جوجه درآوری تخم&shy;ها نسبت به گروه کنترل شد (0.05&gt;P). مصرف خوراک روزانه در مرحله آغازین به طور قابل توجهی در پرندگان در گروه&shy;های 50 و 75 ppm nano-ZnO افزایش یافت (0.05&gt;P). در مرحله رشد در دوره پرورش، نرخ رشد و خوراک مصرفی همه گروه&shy;های تزریق&shy;شده با نانو اکسید روی بالاتر از گروه شاهد بود (0.05&gt;P). تفاوت معنی&shy;داری در ضریب تبدیل خوراک بین گروه&shy;های مختلف مشاهده نشد (0.05&lt;P). نتایج تیتر آنتی&shy;بادی علیه ویروس آنفلوانزا در سن 10 روزگی تفاوتی بین تیمارهای آزمایشی نشان نداد (0.05&lt;P). شمارش هتروفیل، لنفوسیت&shy;ها، مونوسیت&shy;ها و هماتوکریت در خون جوجه&shy;های گوشتی در 10 روزگی اختلاف معنی&shy;داری نشان نداد (0.05&lt;P). نتایج مطالعه حاضر نشان می&shy;دهد که تزریق درون تخم مرغی نانو ذرات ZnO اثرات مثبتی بر میزان مرگ و میر اولیه جنین و کل سلول&shy;های سفید خون داشت، اما عملکرد تولیدی جوجه&shy; های تفریخ &shy;شده به طور معنی&shy;داری تحت تأثیر تزریق درون تخم مرغی نانو ذرات روی قرار نگرفت.

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