Effects of in ovo Injection of Vitamins B6 and B12 in Fertile Eggs Subjected to Ethanol Stress on Hatching Traits, Performance and Visceral Organs of Broiler Chicks Reared under Cold Stress Condition
محورهای موضوعی : Camel
1 - Department of Animal Science, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
2 - Department of Animal Science, College of Agriculture and Natural Resources, Razi University, Kermanshah, Iran
کلید واژه: Performance, broilers, ethanol, vitamins B,
چکیده مقاله :
Two subsequent experiments were conducted to evaluate the effects of in ovo injection of vitamin B6 and B12 in fertile eggs subjected to ethanol (EtOH) stress on hatching traits (first), performance and visceral organs of broiler chicks under cold stress (second). A number of 510 fertile eggs were incubated. A number of 180 eggs were considered as controls (three subgroups as: not-injected, eggshell with a hole and distilled water-injected). A number of 110 eggs were injected with 25 µL of a 1:1 (v/v) mixture of EtOH 47.5% + distilled water. Eggs in two other groups were injected with 25 µL of a 1:1 (v/v) mixture of EtOH 47.5% + 100 µL of B6 (n=110), 25 µL of a 1:1 (v/v) mixture of 47.5% EtOH + 1000 µL of B12 (n=110). A number of 240 one-day chicks, allocated to second experiment. Hatched chicks were divided into 4 treatments. Temperature was maintained 12 ˚C from 28 to 42 days of age. Hatchability percent (P<0.05) reduced by EtOH injected group. The lowest body weight at one day was observed in the EtOH injected group compared to other groups (P<0.05). No significant difference was detected in body weight gain and feed intake of chicks during 1-14 days of age between EtOH + B6 and EtOH + B12 groups. There was no effect of treatment on feed conversion ratio and visceral organ weight (P>0.05). In ovo injection of vitamins B6 and B12 alleviated EtOH-induced oxidative stress in chickens embryos. No significant difference was observed in the performance of the hatched birds in the cold conditions temperature (P>0.05).
در دو آزمایش مجزا اثرات تزریق ویتامینهای ب6 و ب12 در تخم مرغهای بارور تحت تنش تزریق اتانول بر جوجهدرآوری (اول)، عملکرد و اندامهای احشایی جوجههای گوشتی تحت تنش سرمایی طی پرورش (دوم) بررسی شد. تعداد 510 عدد تخم مرغ بارور داخل انکوباسیون قرار گرفتند. تعداد 180 عدد از تخم مرغها، بعنوان شاهد لحاظ شد (شاهد بدون تزریق، شاهد با سوراخ کردن پوسته و شاهد با تزریق آب مقطر). به 110 عدد تخم مرغ، 25 میکرولیتر محلول (1:1) آب مقطر + اتانول 5/47 درصد تزریق گردید. به دو گروه دیگر هر یک به تعداد 110 عدد تخم مرغ، به ترتیب 25 میکرولیتر محلول (1:1) اتانول 5/47 درصد + 100 میکرولیتر ویتامین ب6 و 25 میکرولیتر محلول (1:1) اتانول 5/47 درصد + 1000 میکرولیتر ویتامین ب12 تزریق شدند. از جوجههای تفریخ شده، تعداد 240 قطعه به آزمایش دوم اختصاص یافت. جوجههای تفریخ شده به چهار گروه آزمایشی تقسیم شدند. دمای سالن در 28 تا 42 روزگی در 2 ± 12 درجه سانتیگراد نگهداری گردید. درصد جوجهدرآوری در گروه اتانول به طور معنیداری کاهش یافت (05/0P<). وزن بدن در سن یکروزگی در گروه شاهد در مقایسه با سایر گروهها کمترین بود (05/0P<). تفاوت معنیداری در افزایش وزن بدن و مصرف خوراک در سن 1 تا 14 روزگی بین گروه‌های ب6 و ب12 نبود. تیمارهای آزمایشی بر روی ضریب تبدیل خوراک و وزن اندامهای احشایی تأثیری نداشتند (05/0P>). تزریق ویتامینهای ب6 و ب12 استرس اکسیداتیو را در جنین جوجههای گوشتی بهبود داد. تفاوت معنیداری در عملکرد جوجههای تفریخ شده در شرایط سرما مشاهده نشد.
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