Allelic Variation of MYF5 Gene Detected in the Camelus bactrianus
محورهای موضوعی : Camelن. هدایت-ایوریق 1 , س.ر. میرایی-آشتیانی 2 , م. مرادی شهر بابک 3 , و. واحدی 4 , ح. عبدی 5
1 - Department of Animal Science, Faculty of Agricultural Science, University of Mohaghegh Ardabili, Ardabil, Iran
2 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 - Department of Animal Science, Moghan College Of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
5 - Department of Animal Science, Faculty of Agricultural Science, University of Mohaghegh Ardabili, Ardabil, Iran
کلید واژه: PCR-SSCP, SNP, <, i>, Camelus bactrianus<, /i>, , MYF5 genes,
چکیده مقاله :
The myogenic factors (MYF) 5 gene has been reported to contribute to muscle growth and development, therefore they are considered as candidate genes for growth and meat quality related traits. The MYF5 gene is expressed during proliferation of myoblasts and comprises 3 exons. To ascertain whether there is any variation in the camel MYF5 gene, we have used a polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) method for the analysis. In this study, coding region (exon 1) of the MYF5gene was investigated. Four unique SSCP patterns were detected in exon 1. Two Single nucleotide polymorphisms (SNPs) were detected - A/G and G/A in 98 and 366 position, respectively that create four haplotype, related to banding patterns. The variations detected in this study leads to a serin/asparagine and tryptophan/stop codon amino acid changes, respectively and could be considered for the development of gene-assisted selection in camel breeding.
فاکتور میوژنیک 5 (MYF5) نقش مهمی در رشد و توسعه ماهیچه دارد و به عنوان یک ژن کاندیدا برای صفات رشد و کیفیت گوشت شناخته میشود. ژن MYF5 در دوره تکثیر میوبلاستها بیان میشود که شامل سه اگزون میباشد. به منظور بررسی وجود یا عدم وجود تنوع ژنتیکی در ژن فاکتور میوژنیک 5 در شترهای دو کوهانه ناحیه اگزون 1 ژن MYF5 از روش نشانگر PCR-SSCP استفاده شد. نتایج نشان داد که چهار الگوی متفاوت از اگزون I در نمونههای مورد مطالعه وجود دارد. توالییابی از این الگوها نشان داد که دو نوع چندشکلی تک نوکلئوتیدی (SNP) در موقعیتهای 98 و 366 وجود دارد که به ترتیب منجر به تغییر A به G و G به A میشود این جهشها باعث ایجاد چهار هاپلوتیپ میشود که در ارتباط با الگوهای مختلف شناسایی شده بودند. تنوع و جهش مشاهده شده در این مطالعه منجر به تغییر اسیدآمینه سرین به آسپارژین و تریپتوفان به کدون متوقف کننده میشود که میتوان از آن در توسعه و بهبود برنامههای اصلاح نژادی شتر برای استفاده در انتخاب به کمک ژن به کار گرفته شود.
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