تنوع آللی شناسایی شده ژن MYF5 در شترهای دوکوهانه

Subject Areas : Camel

ن. هدایت-ایوریق ¹ , س.ر. میرایی-آشتیانی ² , م. مرادی شهر بابک ³ , و. واحدی ⁴ , ح. عبدی ⁵

1 - Department of Animal Science, Faculty of Agricultural Science, University of Mohaghegh Ardabili, Ardabil, Iran
2 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
3 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 - Department of Animal Science, Moghan College Of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
5 - Department of Animal Science, Faculty of Agricultural Science, University of Mohaghegh Ardabili, Ardabil, Iran

Received: 2014-10-11 Accepted : 2015-01-31 Published : 2016-06-01

Keywords: PCR-SSCP, SNP, شتر دو کوهانه, ژن MYF5,

Abstract :

فاکتور میوژنیک 5 (MYF5) نقش مهمی در رشد و توسعه ماهیچه دارد و به عنوان یک ژن کاندیدا برای صفات رشد و کیفیت گوشت شناخته می‪شود. ژن MYF5 در دوره تکثیر میوبلاست‪ها بیان می‪شود که شامل سه اگزون می‪باشد. به منظور بررسی وجود یا عدم وجود تنوع ژنتیکی در ژن فاکتور میوژنیک 5 در شترهای دو کوهانه ناحیه اگزون 1 ژن MYF5 از روش نشانگر PCR-SSCP استفاده شد. نتایج نشان داد که چهار الگوی متفاوت از اگزون I در نمونه‪های مورد مطالعه وجود دارد. توالی‪یابی از این الگوها نشان داد که دو نوع چندشکلی تک نوکلئوتیدی (SNP) در موقعیت‪های 98 و 366 وجود دارد که به ترتیب منجر به تغییر A به G و G به A می‪شود این جهش‪ها باعث ایجاد چهار هاپلوتیپ می‪شود که در ارتباط با الگوهای مختلف شناسایی شده بودند. تنوع و جهش مشاهده شده در این مطالعه منجر به تغییر اسید‪آمینه سرین به آسپارژین و تریپتوفان به کدون متوقف کننده می‪شود که می‪توان از آن در توسعه و بهبود برنامه‪های اصلاح نژادی شتر برای استفاده در انتخاب به کمک ژن به کار گرفته شود.

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