Correlation Effects of Nano Selenium and Conjugated Linoleic Acid on the Performance, Lipid Metabolism and Immune System of Male Moghani Lambs
محورهای موضوعی : Camelش. قادرزاده 1 , ف. میرزایی آقجه قشلاق 2 , س. نیک‌بین 3 , نوید شاد 4
1 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
3 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
4 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
کلید واژه: performance, lambs, conjugated linoleic acid, nano selenium, Moghani,
چکیده مقاله :
This study was carried out to evaluate the effects of nano selenium (nano-Se) and conjugated linoleic acid (CLA) on performance, biometric and blood parametersand selenoprotein W1 (SEPW1) and lipid gene expression in male Moghani lambs. Thirty male Moghani lambs, 3-months-old and weighing 30 ± 0.25 kg, were used in completely randomized design in a 2 × 3 factorial arrangement with dietary supplemental the CLA (0 and 15 g/kg DM) and nano-Se (0, 1 and 2 mg/kg DM). The lambs were slaughtered on day 90 (end of experiment). Feeding a mixture of CLA and nano-Se tended to reduce the body weight of lambs comparing to the control group. The experimental diets had no impact on biometric parameters. Some blood parameters like high density lipoprotein (HDL), low density lipoprotein (LDL), thyroxine (T4), triiodothyronine (T3) and glutathione peroxidase (GPx) and glutathione peroxidase depended on dietary nano-Se supplementation (P<0.05); but glucose, triglycerides (TG), very low density lipoprotein (VLDL), total protein (TP) and cholesterol had no considerable effects. The results of qPCR analysis showed that nano-Se supplemental at highest level (2 mg/kg DM) increased gene expression of GPX1 and SEPW1 in liver (P<0.05). Dietary inclusion of CLA enhanced the peroxisome proliferator-activated receptor gamma expression and decreased stearoyl COA desaturase 1 genes in tail (P<0.01). In conclusion, nano-Se and CLA differently increased the gene expression in liver and tail and had good impacts on some blood parameters; suggesting that nano-Se and CLA have not synergism interaction in the above parameters.
این پژوهش اولین مطالعه در رابطه با بررسی اثرات نانوسلنیوم (nano-Se) و اسید لینولئیک مزدوج (CLA) بر روی بیان ژنهای گلوتاتیون پروکسیداز 1 (GPX1) و سلنوپروتئین W1 (SEPW1) در کبد و ژنهای گیرنده متأثر از تکثیر‌کنندههای پروکسیزومی نوع گاما (PPARγ) و استئاروئیل کوانزیم آ دساچوراز 1 (SCD1) در دنبه و فاکتورهای عملکردی، بیومتری و خون برههای نر مغانی میباشد. در این طرح از 30 رأس بره نر مغانی 3 ماهه با وزن 250/0±30 کیلوگرم، در قالب یک طرح کاملاً تصادفی با روش فاکتوریل 3 × 2 با استفاده از مکمل CLA (در سطوح 0 و 15 گرم بر کیلوگرم ماده خشک جیره) و nano-Se (در سطوح 0، 1 و 2 گرم بر کیلوگرم ماده خشک جیره) استفاده گردید. برهها در پایان آزمایش ذبح گردیدند (90 روزگی). مصرف جیرههای آزمایشی در تیمارهای حاوی مخلوط CLA و nano-Se گرایش به کاهش وزن برهها را در مقایسه با تیمار شاهد نشان میداد. هیچ یک از جیرههای آزمایشی اثری بر پارامترهای بیومتری نداشتند. برخی از پارامترهای خونی همچون لیپوپروتئین با چگالی بالا (HDL)، لیپوپروتئین با چگالی کم (LDL)، تیروکسین (T4)، تری یودوتیرونین (T3) و گلوتاتیون پراکسیداز (GPX) تحت تاثیر مکمل nano-Se جیره قرار گرفتند (05/0<P) اما گلوکز، تری گلیسرید (TG)، لیپوپروتئین با چگالی بسیار کم VLDL، پروتئین کل (TP) و کلسترول هیچگونه اثر معنیداری را نشان ندادند. نتایج آنالیز qPCR نشان داد که بالاترین سطح نانوسلنیوم (2 گرم بر کیلوگرم ماده خشک ) به صورت معنیداری باعث افزایش بیان ژن GPX1 و SEPW1 در کبد شده است (05/0>P)). حضور CLA در جیره باعث افزایش بیان ژن PPARγ و کاهش بیان ژن SCD1 در دنبه گردید (01/0>P). از این آزمایش می‌توان نتیجه گرفت که استفاده از nano-Se و CLA به صورت متفاوتی باعث افزایش بیان ژنهای مرتبط به خود در کبد و دنبه میشوند و اثرات مفیدی بر برخی از فاکتورهای خونی دارند که این نتایج اثبات میکند که nano-Se و CLA دارای اثرات متقابل هم‌افزایی بر فاکتورهای مذکور نیستند.
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