اثرات همبستگی نانوسلنیوم و اسید لینولئیک مزدوج بر عملکرد، متابولیسم چربی و سیستم ایمنی برههای نر مغانی
Subject Areas : Camelش. قادرزاده 1 , ف. میرزایی آقجه قشلاق 2 , س. نیک‌بین 3 , نوید شاد 4
1 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
2 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
3 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
4 - Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
Keywords: عملکرد, مغانی, بره, اسید لینولئیک مزدوج, نانوسلنیوم,
Abstract :
این پژوهش اولین مطالعه در رابطه با بررسی اثرات نانوسلنیوم (nano-Se) و اسید لینولئیک مزدوج (CLA) بر روی بیان ژنهای گلوتاتیون پروکسیداز 1 (GPX1) و سلنوپروتئین W1 (SEPW1) در کبد و ژنهای گیرنده متأثر از تکثیر‌کنندههای پروکسیزومی نوع گاما (PPARγ) و استئاروئیل کوانزیم آ دساچوراز 1 (SCD1) در دنبه و فاکتورهای عملکردی، بیومتری و خون برههای نر مغانی میباشد. در این طرح از 30 رأس بره نر مغانی 3 ماهه با وزن 250/0±30 کیلوگرم، در قالب یک طرح کاملاً تصادفی با روش فاکتوریل 3 × 2 با استفاده از مکمل CLA (در سطوح 0 و 15 گرم بر کیلوگرم ماده خشک جیره) و nano-Se (در سطوح 0، 1 و 2 گرم بر کیلوگرم ماده خشک جیره) استفاده گردید. برهها در پایان آزمایش ذبح گردیدند (90 روزگی). مصرف جیرههای آزمایشی در تیمارهای حاوی مخلوط CLA و nano-Se گرایش به کاهش وزن برهها را در مقایسه با تیمار شاهد نشان میداد. هیچ یک از جیرههای آزمایشی اثری بر پارامترهای بیومتری نداشتند. برخی از پارامترهای خونی همچون لیپوپروتئین با چگالی بالا (HDL)، لیپوپروتئین با چگالی کم (LDL)، تیروکسین (T4)، تری یودوتیرونین (T3) و گلوتاتیون پراکسیداز (GPX) تحت تاثیر مکمل nano-Se جیره قرار گرفتند (05/0<P) اما گلوکز، تری گلیسرید (TG)، لیپوپروتئین با چگالی بسیار کم VLDL، پروتئین کل (TP) و کلسترول هیچگونه اثر معنیداری را نشان ندادند. نتایج آنالیز qPCR نشان داد که بالاترین سطح نانوسلنیوم (2 گرم بر کیلوگرم ماده خشک ) به صورت معنیداری باعث افزایش بیان ژن GPX1 و SEPW1 در کبد شده است (05/0>P)). حضور CLA در جیره باعث افزایش بیان ژن PPARγ و کاهش بیان ژن SCD1 در دنبه گردید (01/0>P). از این آزمایش می‌توان نتیجه گرفت که استفاده از nano-Se و CLA به صورت متفاوتی باعث افزایش بیان ژنهای مرتبط به خود در کبد و دنبه میشوند و اثرات مفیدی بر برخی از فاکتورهای خونی دارند که این نتایج اثبات میکند که nano-Se و CLA دارای اثرات متقابل هم‌افزایی بر فاکتورهای مذکور نیستند.
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