اثرات همبستگی نانو‌سلنیوم و اسید لینولئیک مزدوج بر عملکرد، متابولیسم چربی و سیستم ایمنی بره‌های نر مغانی

Subject Areas : Camel

ش.  قادرزاده ¹ (Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran)
ف.  میرزایی آقجه قشلاق ² (Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran)
س.  نیک‌بین ³ (Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran)
نوید  شاد ⁴ (Department of Animal Science, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran)

Received: 2018-06-14 Accepted : 2018-10-30 Published : 2019-09-01

Keywords: عملکرد, مغانی, بره, اسید لینولئیک مزدوج, نانو‌سلنیوم,

Abstract :

این پژوهش اولین مطالعه در رابطه با بررسی اثرات نانو‌سلنیوم (nano-Se) و اسید لینولئیک مزدوج (CLA) بر روی بیان ژن­های گلوتاتیون پروکسیداز 1 (GPX1) و سلنوپروتئین W1 (SEPW1) در کبد و ژن­های گیرنده متأثر از تکثیر‌کننده­های پروکسیزومی نوع گاما (PPARγ) و استئاروئیل کوانزیم آ دساچوراز 1 (SCD1) در دنبه و فاکتورهای عملکردی، بیومتری و خون بره­های نر مغانی می­باشد. در این طرح از 30 رأس بره نر مغانی 3 ماهه با وزن 250/0±30 کیلوگرم، در قالب یک طرح کاملاً تصادفی با روش فاکتوریل 3 × 2 با استفاده از مکمل CLA (در سطوح 0 و 15 گرم بر کیلوگرم ماده خشک جیره) و nano-Se (در سطوح 0، 1 و 2 گرم بر کیلوگرم ماده خشک جیره) استفاده گردید. بره­ها در پایان آزمایش ذبح گردیدند (90 روزگی). مصرف جیره­های آزمایشی در تیمارهای حاوی مخلوط CLA و nano-Se گرایش به کاهش وزن بره­ها را در مقایسه با تیمار شاهد نشان می­داد. هیچ یک از جیره­های آزمایشی اثری بر پارامترهای بیومتری نداشتند. برخی از پارامترهای خونی همچون لیپوپروتئین با چگالی بالا (HDL)، لیپوپروتئین با چگالی کم (LDL)، تیروکسین (T4)، تری یودوتیرونین (T3) و گلوتاتیون پراکسیداز (GPX) تحت تاثیر مکمل nano-Se جیره قرار گرفتند (05/0<P) اما گلوکز، تری گلیسرید (TG)، لیپوپروتئین با چگالی بسیار کم VLDL، پروتئین کل (TP) و کلسترول هیچگونه اثر معنی­داری را نشان ندادند. نتایج آنالیز qPCR نشان داد که بالاترین سطح نانوسلنیوم (2 گرم بر کیلوگرم ماده خشک ) به صورت معنی­داری باعث افزایش بیان ژن GPX1 و SEPW1 در کبد شده است (05/0>P)). حضور CLA در جیره باعث افزایش بیان ژن PPARγ و کاهش بیان ژن SCD1 در دنبه گردید (01/0>P). از این آزمایش می‌توان نتیجه گرفت که استفاده از nano-Se و CLA به صورت متفاوتی باعث افزایش بیان ژن­های مرتبط به خود در کبد و دنبه می­شوند و اثرات مفیدی بر برخی از فاکتورهای خونی دارند که این نتایج اثبات می­کند که nano-Se و CLA دارای اثرات متقابل هم‌افزایی بر فاکتورهای مذکور نیستند.

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