In vitro Evaluation of Oil Releasing Extent from a Calcium Salt of Fatty Acids in Different Sites of Gastrointestinal Tract
محورهای موضوعی : CamelP. Peravian 1 , H. Mirzaei-Alamouti 2 , M. Dehghan-Banadaky 3 , H. Amanlou 4 , M. Vazirigohar 5 , H. Khalilvandi 6 , P. Rezamand 7
1 - Department of Animal Science, University of Zanjan, Zanjan, Iran
2 - Department of Animal Science, University of Zanjan, Zanjan, Iran
3 - Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
4 - Department of Animal Science, University of Zanjan, Zanjan, Iran
5 - Department of Animal Science, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
6 - Department of Animal Science, University of Urmia, Urmia, Iran
7 - Department of Animal and Veterinary Science, University of Idaho, Moscow Idaho 83844-2330, USA
کلید واژه: fish oil, Fatty Acids, Flaxseed oil, Calcium salts, dairy nutrition,
چکیده مقاله :
Calcium salts (CS) of fatty acids (FAs) are involved as fat sources in dairy nutrition. In this supplement dissociation of FAs and calcium is affected by the nature of fatty acids (saturated or unsaturated), so this process led to the release oil in the whole gastrointestinal tract. This study was designed to evaluate the oil releasing extent (ORE) from CS of FAs in a simulated culture of the gastrointestinal tract. Rumen fluid was collected from fistulated cows 4 hours after feeding and transferred to the laboratory. A simulation of the digestion environment at three sites (rumen, abomasums, and small intestine) pH (6.4, 1.2, 6.8) was used to incubate the rumen fluid. Treatments are represented by 1) CS of fish oil (CFO), 2) CS of flaxseed oil (CFL), 3) CS of herbal n-6 and n-3 (CHO) and 4) CS of fish and herbal n-3 (CFH). The ORE in the rumen was affected by treatments, with CS of flaxseed having the greatest rate of release (P<0.01). Differences between treatments in the abomasum were nonsignificant (P=0.1), however, the greatest ORE was in this part in comparison to other sections of the tract. The treatments did affect ORE in the small intestine (P<0.01). According to our results, CS of FAs supplied less than 200 mg/g ORE in the rumen which is acceptable and could be used with minimum adverse effects on rumen function.
Baldin M., Rico D.E., Green M.H. and Harvatine K.J. (2018). Technical note: An in vivo method to determine kinetics of unsaturated fatty acid biohydrogenation in the rumen J. Dairy Sci. 101, 1-9.
Carroll S.M., DePeters E.J. and Rosenberg M. (2006). Efficacy of a novel whey protein gel complex to increase the unsaturated fatty acid composition of bovine milk fat. J. Dairy Sci. 89, 640-651.
Chilliard Y., Glasser F., Ferlay A., Bernard L., Rouel J. and Doreau M. (2007). Diet, rumen biohydrogenation and nutritional quality of cow and goat milk fat. European J. Lipid Sci. Technol. 109, 828-855.
De Souza J., Preseault C.L. and Lock A.L. (2018). Altering the ratio of dietary palmitic, stearic, and oleic acids in diets with or without whole cottonseed affects nutrient digestibility, energy partitioning, and production responses of dairy cows. J. Dairy Sci. 101, 172-185.
Dewhurst R.J. and Moloney A.P. (2013). Modification of animal diets for the enrichment of dairy and meat products with omega-3 fatty acids. Pp. 257-287 in Food Enrichment with Omega-3 Fatty Acids. C. Jacobsen, N. Skall Nielsen, A. Frisenfeldt Horn and A.D. Moltke Sørensen, Eds. Woodhead Publishing Ltd, Cambridge, United Kingdom.
Fievez V., Vlaeminck B., Jenkins T., Enjalbert F. and Doreau M. (2007). Assessing rumen biohydrogenation and its manipulation in vivo, in vitro and in situ. European J. Lipid Sci. Technol. 109, 740-756.
Givens D.I. and Shingfield K.J. (2006). Optimizing dairy milk fatty acid composition. Pp. 252-280 in Improving the fat content of foods. C.M. Williams and J. Buttriss, Eds. Woodhead Publishing Ltd, Cambridge, United Kingdom.
Khalilvandi H., Dehghan-banadaki M. and Ghafarzadeh M. (2013). In vitro assessment of different protection methods of fish oil. J. Rumin. Res. 4, 1-28.
Kosaraju S.L., Weerakkody R. and Augustin M.A. (2009). In vitro evaluation of hydrocolloid-based encapsulated fish oil. Food Hydrocol. 23, 1413-1419.
Loor J., Ueda J., Ferlay K., Chilliard A.Y. and Doreau M. (2004). Biohydrogenation, duodenal flow, and intestinal digestibility of trans fatty acids and conjugated linoleic acids in response to dietary forage: Concentrate ratio and linseed oil in dairy cows. J. Dairy Sci. 87, 2472-2485.
NRC. (2001). Nutrient Requirements of Dairy Cattle. 7th Ed. National Academy Press, Washington, DC., USA.
Rabiee A.R., Breinhild K., Scott W., Golder H.M., Block E. and Lean I.J. (2012). Effect of fat additions to diets of dairy cattle on milk production and components: A meta-analysis and meta-regression. J. Dairy Sci. 95, 3225-3247.
SAS Institute. (2003). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Silvestre F.T., Carvalho T.S.M., Crawford C., Santos J.E.P., Staples C.R. and Thatcher W.W. (2008). Effects of differential supplementation of calcium salts of fatty acids (CSFAs) to lactating dairy cows on plasma acute phase proteins and leukocyte responses: Phagocytic and oxidative burst, CD62L and CD18 expression and cytokine production. J. Biol. Reprod. 2008, 1-158.
Sinclair L.A., Cooper S.L., Chikunya S., Wilkinson R.G., Hallett K.G., Enser M. and Wood J.D. (2005). Biohydrogenation of n-3 polyunsaturated fatty acids in the rumen and their effects on microbial metabolism and plasma fatty acid concentrations in sheep. J. Anim Sci. 81, 239-248.
Vazirigohar M., Dehghan-Banadaki M., Rezayazdi K., Krizan S.J., Nejati-Javaremi A. and Shingfield K.J. (2014). Fat source and dietary forage to concentrate ratio influences milk fatty acid composition in lactating cows. Animal. 8, 163-174.
