Analysis of Awassi Sperm Motility in Two Media at Different Levels of Temperature, pH and Osmolality
محورهای موضوعی : Camel
م. آلومار
1
(Department of Agriculture, Atomic Energy Commission, Damascus, Syria)
م. زرکاوی
2
(Department of Agriculture, Atomic Energy Commission, Damascus, Syria)
م.آ. آلزوآبی
3
(Department of Agriculture, Atomic Energy Commission, Damascus, Syria)
کلید واژه: temperature, ram, computer-aided sperm analyzer, osmolality,
چکیده مقاله :
The effects of five levels of osmolality and pH as well as three temperatures on Awassi ram sperm motility parameters assessed by computer-aided sperm analyzer(CASA)in tyrode albumin lactate pyruvate (TALP) and egg-yolk-tris (YET) media were studied. Semen obtained from five adult Awassi rams were pooled and diluted in the above two media at 100, 200, 300, 400 and 500 mOsm/kg at temperatures of 4˚, 20˚ and 37˚ degree celsius and also at pH levels of 5, 6, 7, 8 and 9 at the same temperatures. The osmolality, pH and temperature had a significant effect (P<0.05) on the percent motility (MOT %) and the percent of sperm showing progressive motility (PMOT %). With the exception of lateral head displacement, all the other CASA motility parameters were also significantly affected (P<0.05). Semen incubated in YET was able to tolerate osmolarities within 200 to 400 mOsm/kg range for MOT %. Alkaline condition at pH 9 had higher negative effect than acidic condition at pH 5. Compared to the 20 ˚C and 37 ˚C, the 4 ˚C had negatively affected MOT % and PMOT % and this was more obvious in TALP medium. In conclusion, our results suggest: 1) the significant effects of osmolality, pH and temperature on Awassi sperm incubated in both TALP and YET media and 2) the need for careful selection of temperature by which the semen may be manipulated during artificial insemination and in vitro fertilization experiments in these two media.
اثرات پنج سطح اسمولاریته و pH همراه با سه دمای مختلف روی پارامترهای تحرک اسپرم قوچهای آواسی با استفاده از تحلیلگر اسپرم مبتنی بر کامپیوتر (CASA) در محیط کشتهای تیرود آلبومین لاکتات پیرووات (TALP) و تریس زرده تخم مرغ (YET) مطالعه گردید. اسپرم حاصل از 5 قوچ آواسی بالغ با هم مخلوط گردیده و روی دو محیط با میلیاسمولاریته 100، 200، 300، 400 و 500 به ازای هر کیلوگرم در دمای 4، 20 و 37 درجه سلسیوس و نیز در سطح pH 5، 6، 7، 8 و 9 در دماهای یکسان رقیق گردیدند. اسمولاریته، pH و دما اثر معنیداری (05/0>P) روی درصد تحرک (MOT %) و درصد اسپرم با حرکت پیشرونده (PMOT %) داشتند. غیر از ناهنجاری سر جانبی، همه پارامترهای دیگر تحرک CASA نیز به طور معنیداری تحت تأثیر قرار گرفتند (05/0>P). اسپرم انکوباسیون شده در YET قادر بود در اسمولاریتههای بین 200 تا 400 میلیاسمول به ازای هر کیلوگرم دامنه برای MOT % مقاومت کند. شرایط بازی در 9 =pH اثر منفی بزرگتری در مقایسه با شرایط اسیدی در 5 =pH داشت. در مقایسه با 20 و 37 درجه سلسیوس، دمای 4 درجه سلسیوس تأثیر منفی بر MOT % و PMOT % داشته و این امر در محیط TALP با وضوح بیشتری دیده میشد. به عنوان نتیجهگیری، نتایج ما نشان داد که: 1) اثرات اسمولاریته، pH و دما بر اسپرم آواسی انکوباسیون شده در هر دو محیط TALP و YET معنی‌دار بود و 2) لازم است که دما برای اسپرمی که در تلقیح مصنوعی و آزمایشات باروری خارج رحمی در این دو محیط مورد استفاده قرار میگیرند، به دقت انتخاب شوند.
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