آنالیز تحرک اسپرم آواسی در دو محیط در سطوح مختلف دما، pH و اسمولاریته

Subject Areas : Camel

م. آلومار ¹ (Department of Agriculture, Atomic Energy Commission, Damascus, Syria)
م. زرکاوی ² (Department of Agriculture, Atomic Energy Commission, Damascus, Syria)
م.آ. آلزوآبی ³ (Department of Agriculture, Atomic Energy Commission, Damascus, Syria)

Received: 2017-11-02 Accepted : 2017-12-31 Published : 2018-09-01

Keywords: دما, قوچ, تحلیل‌گر اسپرم مبتنی بر کامپیوتر, اسمولاریته,

Abstract :

اثرات پنج سطح اسمولاریته و pH همراه با سه دمای مختلف روی پارامترهای تحرک اسپرم قوچ­های آواسی با استفاده از تحلیل­گر اسپرم مبتنی بر کامپیوتر (CASA) در محیط کشت­های تیرود آلبومین لاکتات پیرووات (TALP) و تریس زرده تخم مرغ (YET) مطالعه گردید. اسپرم حاصل از 5 قوچ آواسی بالغ با هم مخلوط گردیده و روی دو محیط با میلی­اسمولاریته 100، 200، 300، 400 و 500 به ازای هر کیلوگرم در دمای 4، 20 و 37 درجه سلسیوس و نیز در سطح pH 5، 6، 7، 8 و 9 در دماهای یکسان رقیق گردیدند. اسمولاریته، pH و دما اثر معنی­داری (05/0>P) روی درصد تحرک (MOT %) و درصد اسپرم با حرکت پیش­رونده (PMOT %) داشتند. غیر از ناهنجاری سر جانبی، همه پارامترهای دیگر تحرک CASA نیز به طور معنی­داری تحت تأثیر قرار گرفتند (05/0>P). اسپرم انکوباسیون شده در YET قادر بود در اسمولاریته­های بین 200 تا 400 میلی­اسمول به ازای هر کیلوگرم دامنه برای MOT % مقاومت کند. شرایط بازی در 9 =pH اثر منفی بزرگتری در مقایسه با شرایط اسیدی در 5 =pH داشت. در مقایسه با 20 و 37 درجه سلسیوس، دمای 4 درجه سلسیوس تأثیر منفی بر MOT % و PMOT % داشته و این امر در محیط TALP با وضوح بیشتری دیده می­شد. به عنوان نتیجه­گیری، نتایج ما نشان داد که: 1) اثرات اسمولاریته، pH و دما بر اسپرم آواسی انکوباسیون شده در هر دو محیط TALP و YET معنی‌دار بود و 2) لازم است که دما برای اسپرمی که در تلقیح مصنوعی و آزمایشات باروری خارج رحمی در این دو محیط مورد استفاده قرار می­گیرند، به دقت انتخاب شوند.

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