Insulin Like Growth Factor-1 Fortification Improves Post Thaw Sperm Quality and Fertility of Goat
محورهای موضوعی : Camel
K. Sharma
1
(Department of Animal Physiology and Reproduction, Institute of Indian Council of Agricultural Research (ICAR)-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh, India|GLA University, Mathura, UP, India)
R. Ranjan
2
(Department of Animal Physiology and Reproduction, Institute of Indian Council of Agricultural Research (ICAR)-Central Institute for Research on Goats, Farah, Mathura, Uttar Pradesh, India)
S. Gupta
3
(GLA University, Mathura, UP, India)
کلید واژه: Antioxidant, Malondialdehyde, IGF-1, Goat, acrosome,
چکیده مقاله :
Insulin like growth factor-1 (IGF-I) has an antioxidant effect to maintain sperm motility. The present study designed to evaluate the effect of different levels IGF-1 on semen characteristic after cryopreservation of buck semen. Fifty ejaculates were collected and were extended with tris- citric acid- fructose diluent with 10% egg yolk and 6% glycerol with sperm concentrations of 4 × 108 mL−1. The effect of IGF-1 concentration showed significant differences (P<0.05) on most of studied traits (sperm motility, live sperm count, acrosomes integrity, hypo osmotic swelling positive spermatozoa, malondialdehyde (MDA)) and were significantly (P<0.05) highest in 250 ng/mL of IGF-1in post thaw semen. The post thaw progressive motility, live, hypo osmotic swelled and acrosome intact sperm increased by 25.26%, 26.77%, 30.35% and 25.52%, respectively in 250 ng/mL IGF1supplemented group in compare with control group. The 250 ng/mL IGF-1 supplemented group showed the lowest MDA content (P<0.05) in compare with other studied groups. The fortification 250 ng/mL IGF-1 reduced the MDA content by 31.16% more than the control group. The results suggested that IGF-1 protects sperm membrane lipid peroxidation by lowering MDA production by reducing the harmful effect of reactive oxygen species. Eight out of ten goats were conceived and kidded by using supplemented of 250 ng/mL IGF-1 and three goats were kidded by non-supplemented (control) frozen semen straw through artificial insemination (AI). The success of kidding was 40.00% in supplemented group (250 ng/mL IGF-1) than 30.00% in non-supplemented group (control) on actual kidding basis.
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