Quantitative Trait Loci for some of Behavior and Performance Traits on Chromosome 4 of Japanese Quail
محورهای موضوعی : Camelا. رضوان نژاد 1 , ا. نصیری فر 2
1 - Department of Biotechnology, Institute Science and High Technology and Environmental Science, Graduate University of Advanced Technology, Kerman, Iran
2 - Department of Biotechnology, Institute Science and High Technology and Environmental Science, Graduate University of Advanced Technology, Kerman, Iran
کلید واژه: Japanese quail, tonic immobility, QTL mapping, body weight trait, daily gain trait,
چکیده مقاله :
The current study was conducted to identify the quantitative trait locus (QTL) for the body weight at age 1, 7, 14, 21 and 28 days and daily gain at age 0-1, 1-2, 2-3 and 3-4 weeks, slighter carcass weight and tonic immobility in Japanese quail. Two divergently lines of wild and white Japanese quail which maintained in the Animal Science Research Center of the Shahid Bahonar University of Kerman was used. Birds in two lines were mated in reciprocal cross for generating F1 generation. The F2 generation was generated by random mating of F1 birds. The half sib and F2 model analysis of QTL express was used for QTL mapping the measured traits. Total F2 individuals (422 birds), their parents (34 F1 birds) and pure lines (16 birds) were genotyped by 2 informative microsatellite markers on chromosomes 4. On this chromosome, with the half sib model, no significant QTL at the 5%, 1% chromosome wide was detected. But, with the F2 model, a significant QTL at the 5% chromosome wide was identified for body weight (BW) at age 21 and 28 days, carcass weight and daily gain traits during 1-2, 2-3 and 3-4 weeks in 54cM position. The all of identified QTLs explain less of 4% of the phenotypic variance, because the traits of growth are complex traits affected by many loci influencing appetite, feed uptake, nutrient allocation, metabolic rate, physical activity and so forth.
مطالعه حاضر به منظور شناسایی وجود QTL برای صفات وزن بدن در سنین 1، 7، 14، 21 و 28 روزگی، نرخ رشد روزانه در سنین 1-0، 2-1، 3-2 و 4-3 هفتگی، وزن لاشه بعد از کشتار و عدم تحرک در بلدرچین ژاپنی انجام شد. دو لاین متمایز بلدرچین ژاپنی شامل لاین وحشی و سفید که در مزرعه تحقیقاتی دانشگاه شهید باهنر کرمان نگهداری میشدند، مورد استفاده قرار گرفتند. پرندگان دو لاین به صورت تلاقی جابجایی برای تولید نسل F1 با یکدیگر تلاقی داده شدند. نسل F2 از تلاقی تصادفی پرندگان نسل F1 تولید شدند. مدل مورد استفاده برای نقشه یابیQTL برای صفات اندازهگیری شده، مدل F2 و ناتنی‌ها در نرم افزار QTL express بود. کل افراد نسل F2 (422 پرنده)، والدین آنها )34 پرنده (F1و لاین‌های خالص (16 پرنده) توسط 2 نشانگر ریزماهواره بر روی کروموزوم 4 ژنوتایپ شدند. بر روی این کروموزوم، با استفاده از مدل ناتنی، QTL معنی‌داری در سطح 05/0 و 01/0 کروموزوم، شناسایی نشد. اما با مدل F2، یک QTL معنی‌دار در سطح 05/0کروموزوم برای وزن بدن در سن 21 و 28 روزگی، وزن لاشه ونرخ رشد روزانه در طی 2-1، 3-2 و 4-3 هفتگی در موقعیت 54cM شناسایی شد. از آنجاکه صفات رشد صفات پیچیدهای هستند که تحت تأثیر بسیاری از جایگاه‌های مؤثر بر اشتها، جذب غذا، تخصیص مواد مغذی، میزان سوخت و ساز، فعالیت بدنی و غیره می‌باشند، همهQTL های شناسایی شده کمتر از 4 درصد از واریانس فنوتیپی برای صفات مورد نظر توضیح میدهند.
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