Using 15N Dilution Method to Correct for Microbial Contamination When Assessing in situ Protein Degradability of Fresh Ryegrass

محورهای موضوعی : Camel

ر. طهماسبی ¹ , ج. و. نولان ² , ر. س. دوبس ³

1 - Department of Animal Science, Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran|School of Environmental and Rural Science, University of New England, New South Wales, Armidale, Australia
2 - School of Environmental and Rural Science, University of New England, New South Wales, Armidale, Australia
3 - Department of Primary Industries, Beef Industry Centre of Excellence, New South Wales, Armidale, Australia

تاریخ دریافت : 1394/03/22 تاریخ پذیرش : 1394/10/10 تاریخ انتشار : 1395/03/12

کلید واژه: Degradation, ryegrass, Microbial contamination, labeled nitrogen, &lt, i&gt, in situ&lt, /i&gt, ,

چکیده مقاله :

This experiment was done to investigate microbial contamination and in situ disappearance rates of dry matter (DM), N and 15N of fresh labeled ryegrass. Perennial ryegrass (Lolium perenne) were labeled with 15N during growth in a glasshouse, harvested at 4th leaves stage and were incubated up to 34 h in situ in the rumen of 3 individually housed sheep. The animals were fed 800 g/d chopped alfalfa and had free access to drinking water. Six bags were placed in the rumen of each sheep simultaneously and removed after 0, 3, 7, 12, 21 and 33 h after incubation. The results were fitted to a model describing the degradation of DM and total N with time. It was found that residues from the washed zero time bags had lower 15N enrichments (7.7% vs. 8.3% enriched) than the original fresh samples. Under-estimation of effective degradability (ED) of protein in fresh forages by about 4% would have potential consequences for predictions of ruminally fermentable and escape protein and thus for dietary protein feeding management. However, because the correction assumes contaminating microbial N is unlabeled, but microbes attached to labeled ryegrass would become labeled to some extent, the true error and effective degradability may still be underestimated. Studies with two markers would help us to better understand the errors associated with the in situ technique.

چکیده انگلیسی:

این آزمایش برای تعیین میزان آلودگی میکروبی و نرخ‪های ناپدید شدن ماده خشک، نیتروژن و نیتروژن 15 در گیاه نشان‪دار شده رای‪گراس صورت گرفت. پرنیال رای‪گراس (لولیوم پرنه) با استفاده از محلول نیتروژن 15 در هنگام رشد در گلخانه نشان‪دار گردید و در مرحله چهار برگی برداشت شد. سپس به مدت 33 ساعت درون شکمبه سه رأس گوسفند فیستولا شده، که به صورت مجزا در قفس‪های متابولیکی نگهداری می‪شدند، قرار داده شد. گوسفندان روزانه 800 گرم یونجه خرد شده دریافت می‪کردند و به آب آشامیدنی تازه دسترسی داشتند. تعداد 6 کیسه به صورت همزمان درون شکمبه گوسفندان قرار داده شد و سپس در زمان‪های 0، 3، 7، 12، 21 و 33 ساعت پس از شکمبه‪گذاری برداشته شدند. نتایج با استفاده از یک مدل نشان دهنده تجزیه‪پذیری ماده خشک و کل نیتروژن در زمان تنظیم گردیدند. نتایج نشان داد که بقایای حاصل از شسته شدن کیسه‪های زمان صفر در مقایسه با نمونه‪های گیاه تازه، درصد غنی شدن پایینتری داشتند (7/7 درصد در مقایسه با 3/8 درصد). تخمین کمتر از حد تجزیه‪پذیری مؤثر پروتئین گیاه تازه در حدود 4 درصد می‪تواند در باره پیش‪بینی پروتئین عبوری و تجزیه‪پذیر در شکمبه و همینطور مدیریت تغذیه پروتئین پیامدهایی داشته باشد. بنابراین به دلیل این که تصحیح نمودن بر پایه این فرض استوار می‪باشدکه نیتروژن میکروبی نشان‪دار نمی‪باشد اما میکروب‪ها با چسبیدن به رای‪گراس تا اندازه‪ای نشان‪دار می‪شوند، خطای واقعی و تجزیه‪پذیری مؤثر کماکان ممکن است کمتر از حد تخمین زده شوند. انجام مطالعات با دو مارکر می‪تواند برای درک بهتر خطاهای مرتبط با تکنیک کیسه‪های نایلونی کمک شایانی نماید.

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