Osmotic Tolerance and Freezability of Beetal Goat Sperm during Breeding and Non-Breeding Seasons
محورهای موضوعی : Camel
M. Akhtar
1
(
Department of Molecular Biology, Faculty of Science and Technology, Virtual University, Lahore, Pakistan
)
E. Ahmad
2
(
Department of Clinical Science, Faculty of Veterinary Science, Bahauddin Zakariya University, Multan, Pakistan
)
Z. Naseer
3
(
Department of Clinical Science, Faculty of Veterinary and Animal Science, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
)
M.S. Akhtar
4
(
Department of Clinical Science, Faculty of Veterinary Science, Bahauddin Zakariya University, Multan, Pakistan
)
T. Ahmad
5
(
Department of Clinical Science, Faculty of Veterinary Science, Bahauddin Zakariya University, Multan, Pakistan
)
T. Hussain
6
(
Department of Molecular Biology, Faculty of Science and Technology, Virtual University, Lahore, Pakistan
)
M.T. Khan
7
(
Department of Clinical Science, Faculty of Veterinary Science, Bahauddin Zakariya University, Multan, Pakistan
)
کلید واژه: Season, freezing, Beetal goat sperm, osmotic tolerance,
چکیده مقاله :
The present study was conducted to determine seasonal variations in osmotic tolerance and post-thaw quality of Beetal goat sperm. The ejaculates (n=12) were collected from mature bucks (n=3) during breeding (late summer-early fall) and nonbreeding (winter-early spring) seasons. In the first experiment, the samples were exposed to hypo-osmotic, iso-osmotic, and hyper-osmotic conditions adjusted to 50, 100, 290, 500, or 1000 mOsm/L and incubated at 37 ˚C for 15 min. After the osmotic challenge, sperm viability and plasma membrane integrity (PMI) were determined using a combined hypo-osmotic swelling test (HOST) and eosin-nigrosine staining. In experiment 2, the ejaculates were diluted with a tris-citric acid fructose (TCF) extender and frozen in liquid nitrogen following a standard protocol. After freezing, samples were thawed to assess motility, motion kinetics, PMI, and viability. The results showed a significant (P<0.05) impact of the interaction between osmolality and season on live-HOST +ve and total-HOST +ve sperm. The live-HOST +ve and total-HOST +ve sperm were higher (P<0.05) in the breeding season. Post-thaw sperm PMI, progressive and total motilities were higher (P<0.05) in the breeding season; while, viability and sperm motion kinetics were similar (P>0.05) between the seasons. In conclusion, the season impacts the osmotic tolerance and cryopreservation of Beetal goat sperm.
Ahmad E., Naseer Z., Uçan U., Serin İ., Ceylan A. and Aksoy M. (2018). Seasonal variations in sperm acrosome reaction, osmotic tolerance and serum testosterone concentrations in rams. Anim. Reprod. Sci. 198, 112-120.
Arrebola F. and Abecia J.A. (2017). Effects of season and artificial photoperiod on semen and seminal plasma characteristics in bucks of two goat breeds maintained in a semen collection center. Vet. World. 10, 521-525.
Gallego-Calvo L., Gatica M.C., Santiago-Moreno J., Guzman J.L. and Zarazaga L. (2015). Seasonal changes in reproductive activity, sperm variables and sperm freezability in Blanca Andaluza bucks. Spanish J. Agric. Res. 13, e0403.
Gloria A., Henning H., Di Francesco L. and Contri A. (2021). Osmotic tolerance of rabbit spermatozoa is affected by extender composition and temperature. Anim. Reprod. Sci. 229, 11-21.
Johnston S.D., Lever J., McLeod R., Qualischefski E., Brabazon S., Walton S. and Collins S.N. (2014). Extension, osmotic tolerance and cryopreservation of saltwater crocodile (Crocodylus porosus) spermatozoa. Aquaculture. 426, 213-221.
Küçük N., Aksoy M., Uçan U., Ahmad E., Naseer Z., Ceylan A. and Serin I. (2014). Comparison of two different cryopreservation protocols for freezing goat semen. Cryobiology. 68, 327-331.
Kulaksız R., Arı U.Ç., Kuru M., Yıldız S., Lehimcioğlu N.C., Öztürkler Y. and Atakişi E. (2019). Seasonal changes in testes size, fresh and post-thawing semen characteristics, serum testosterone level, and phospholipase A2 activity in Gurcu male goats. J. Anim. Plant Sci. 29, 353-358.
Kulaksiz R., Ari U.C., Kuru M., Yildiz S., Lehimcioğlu N.C. and Öztürkler Y. (2020). Seasonal variations in testicular measurements, fresh sperm quality and post-thaw sperm motility in Gurcu goat bucks. Slovak J. Anim. Sci. 53, 161-167.
Kumar N., Rai B., Bhat S.A., Kharche S.D., Gangwar C., Jindal S.K. and Chandra S. (2016). Effect of management system and season on semen freezability in Jakhrana bucks. Vet. World. 9, 199-202.
Kunkitti P., Chatdarong K., Suwimonteerabutr J., Nedumpun T., Johannisson A., Bergqvist A.S., Sjunnesson Y. and Axnér E. (2017). Osmotic tolerance of feline epididymal spermatozoa. Anim. Reprod. Sci. 185, 148-153.
Oldenhof H., Heutelbeck A., Blässe A.K., Bollwein H., Martinsson G., Wolkers W.F. and Sieme H. (2015). Tolerance of spermatozoa to hypotonic stress: role of membrane fluidity and correlation with cryosurvival. Reprod. Fertil. Dev. 27, 285-293.
Qureshi M.S., Khan D., Mustaq A. and Afridi S.S. (2013). Effect of extenders, post dilution intervals and seasons on semen quality in dairy goats. Turkish J. Vet. Anim. Sci. 35, 147-152.
Ranjan R., Singh P., Gangwar C., Singh S., Swain D. and Kharche S. (2021). Fortification of catalase improves post-thaw fertility of goat semen. Iranian J. Appl. Anim. Sci. 11, 587-593.
Ustuner B., Gokce E., Toker M.B., Onder N.T., Soylu M.K., Sagirkaya H. and Nur Z. (2018). Effect of sperm pooling with seminal plasma collected in breeding or nonbreeding season on Saanen goat sperm cryo survival. Andrologia. 50, e12968.
Wang W., Luo J., Sun S., Xi L., Gao Q., Haile A.B., Shi H., Zhang W. and Shi H. (2015). The effect of season on spermatozoa motility, plasma membrane and acrosome integrity in fresh and frozen thawed semen from Xinong Sanen bucks. Reprod. Dom. Anim. 50, 23-28.
Xu B., Wang R., Wang Z., Liu H., Wang Z., Zhang W., Zhang Y., Su R., Liu Z., Liu Y., Li J. and Zhang J. (2022). Evaluation of lipidomic change in goat sperm after cryopreservation. Front. Vet. Sci. 9, 1-11.
