Osmotic Tolerance and Freezability of Beetal Goat Sperm during Breeding and Non-Breeding Seasons
محورهای موضوعی : Camel
M. Akhtar
1
,
E. Ahmad
2
,
Z. Naseer
3
,
M.S. Akhtar
4
,
T. Ahmad
5
,
T. Hussain
6
,
M.T. Khan
7
1 - Department of Molecular Biology, Faculty of Science and Technology, Virtual University, Lahore, Pakistan
2 - Department of Clinical Science, Faculty of Veterinary Science, Bahauddin Zakariya University, Multan, Pakistan
3 - Department of Clinical Science, Faculty of Veterinary and Animal Science, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan
4 - Department of Clinical Science, Faculty of Veterinary Science, Bahauddin Zakariya University, Multan, Pakistan
5 - Department of Clinical Science, Faculty of Veterinary Science, Bahauddin Zakariya University, Multan, Pakistan
6 - Department of Molecular Biology, Faculty of Science and Technology, Virtual University, Lahore, Pakistan
7 - Department of Clinical Science, Faculty of Veterinary Science, Bahauddin Zakariya University, Multan, Pakistan
کلید واژه: Season, freezing, Beetal goat sperm, osmotic tolerance,
چکیده مقاله :
The present study was conducted to determine seasonal variations in osmotic tolerance and post-thaw quality of Beetal goat sperm. The ejaculates (n=12) were collected from mature bucks (n=3) during breeding (late summer-early fall) and nonbreeding (winter-early spring) seasons. In the first experiment, the samples were exposed to hypo-osmotic, iso-osmotic, and hyper-osmotic conditions adjusted to 50, 100, 290, 500, or 1000 mOsm/L and incubated at 37 ˚C for 15 min. After the osmotic challenge, sperm viability and plasma membrane integrity (PMI) were determined using a combined hypo-osmotic swelling test (HOST) and eosin-nigrosine staining. In experiment 2, the ejaculates were diluted with a tris-citric acid fructose (TCF) extender and frozen in liquid nitrogen following a standard protocol. After freezing, samples were thawed to assess motility, motion kinetics, PMI, and viability. The results showed a significant (P<0.05) impact of the interaction between osmolality and season on live-HOST +ve and total-HOST +ve sperm. The live-HOST +ve and total-HOST +ve sperm were higher (P<0.05) in the breeding season. Post-thaw sperm PMI, progressive and total motilities were higher (P<0.05) in the breeding season; while, viability and sperm motion kinetics were similar (P>0.05) between the seasons. In conclusion, the season impacts the osmotic tolerance and cryopreservation of Beetal goat sperm.
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