Distribution of Allele Frequencies at 5′-Flanking Region of CYP19 and ERα Genes between Iranian Simmental and Three Indigenous Cattle Breeds

محورهای موضوعی : Camel

ف. محمد نژاد سنگدهی ¹ (Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agriculture science and Natural Resources University, Sari, Iran)
ق. رحیمی میانجی ² (Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agriculture science and Natural Resources University, Sari, Iran)
م. صفدری شاهرودی ³ (Department of Animal Science, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj, Iran)
س.ع. رضوی-ششده ⁴ (Department of Animal Science, Faculty of Animal Science and Fishery, Sari Agriculture science and Natural Resources University, Sari, Iran)
م. غلامی ⁵ (Department of Animal Science, Faculty of Agricultural Science and Engineering, University of Tehran, Karaj, Iran)

تاریخ دریافت : 1393/02/17 تاریخ پذیرش : 1393/05/23 تاریخ انتشار : 1394/03/11

کلید واژه: cattle breeds, CYP19 gene, ERα gene, estrogen, polymorphisms, promoter,

چکیده مقاله :

This study was performed to investigate two polymorphic sites from Cyp19 gene (PvuII and MspI) and one polymorphic site from ERα gene (SnaBI) in four cattle breeds including Mazandarani, Taleshi, Sistani and Simmental. In overall 278 samples for CYP19 and 206 samples for ERα marker sites were genotyped using polymerase chain reactionsingle-strand conformation polymorphism (PCR-RFLP) procedure. For CYP19/PvuII, the frequency of A allele ranged from 0.89 in Mazandarani to 0.98 in Taleshi. For CYP19/MspI, Taleshi was the only monomorphic breed with just AA genotype. Other breeds were polymorphic with A allele frequency ranging from 0.93 (Simmental) to 0.98 (Sistani). No BB individuals were observed in the studied sample. Considering two loci in combination with each other, only 5 out of 8 theoretically possible combined genotypes were observed in the genotyped samples. For ERα/SnaBI, all populations showed polymorphism. The highest and lowest frequencies of A allele were observed in Sistani (0.55) and Simmental (0.96), respectively. Based on Nei’s method, the most genetic distance was observed between Sistani and Simmental and the least genetic distance was observed between Mazandarani and Taleshi breeds. The results of the present study showed significant differences in genotypic and allelic frequencies among four investigated breeds. These findings may be used in further researches, such as association studies for performance traits in cattle breeding.

چکیده انگلیسی:

پژوهش حاضر به منظور بررسی دو جایگاه پلی مورف از ژن CYP19 (PvuII و MspI) و یک جایگاه پلی مورف از ژن ERα (SnaBI) در چهار نژاد گاو شامل مازندرانی، تالشی، سیستانی و سیمنتال انجام شد. در مجموع 278 نمونه برای ژن CYP19 و 206 نمونه برای ژن ERα با استفاده از تکنیک PCR-RFLP تعیین ژنوتیپ شدند. برای جایگاه CYP19/PvuII، فراوانی آلل A دامنه­ای از 89/0 در نژاد مازندرانی تا 98/0 در نژاد تالشی داشت. برای جایگاه CYP19/MspI، تالشی تنها نژاد مونو مورف با ژنوتیپ AA بود. سایر نژادها پلی مورف بودند و فراوانی آلل A از 93/0 (سیمنتال) تا 98/0 (سیستانی) متغیر بود. ژنوتیپ BB در نمونه مورد مطالعه مشاهده نشد. با در نظر گرفتن دو جایگاه در ترکیب با یکدیگر، تنها 5 ژنوتیپ از 8 ژنوتیپ ترکیبی ممکن مشاهده شد. برای جایگاه ERα/SnaBI، همه جمعیت­ها پلی مورف بودند. بیشترین و کمترین فراوانی آلل A به ترتیب در نژادهای سیستانی (55/0) و سیمنتال (96/0) مشاهده شد. بر اساس شاخص نی، بیشترین فاصله ژنتیکی بین دو نژاد سیستانی و سیمنتال، و کمترین فاصله ژنتیکی بین دو نژاد مازندرانی و تالشی مشاهده شد. نتایج پژوهش حاضر نشان دهنده وجود اختلاف معنی دار در فراوانی­های ژنوتیپی و آللی در بین چهار نژاد مورد بررسی بود. این یافته­ها ممکن است در مطالعات آتی مانند مطالعات ارتباطی برای صفات عملکردی در اصلاح نژاد گاو مورد استفاده قرار گیرد.

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