Genetic Analysis of Nigerian Indigenous Goat Populations Using Microsattelite Markers

محورهای موضوعی : Camel

اُ.آ. اُجو ¹ ( National Agricultural Extension and Research Liaison Services, Ahmadu Bello University, Zaria, Nigeria )
جی.ن. آکپا ² ( Department of Animal Science, Ahmadu Bello University, Zaria, Nigeria )
م. اُرونمویی ³ ( Department of Animal Science, Federal University, Oye-Ekiti, Nigeria )
آی.آ. آدیینکا ⁴ ( National Animal Production Reseacrch Institute, Ahmadu Bello University, Zaria, Nigeria )
م. کبیر ⁵ ( Department of Animal Science, Ahmadu Bello University, Zaria, Nigeria )
سی. آلفونسوس ⁶ ( Department of Animal Science, Kaduna State University, Kafanchan Campus, Nigeria )

تاریخ دریافت : 1396/04/06 تاریخ پذیرش : 1396/06/24 تاریخ انتشار : 1397/03/11

کلید واژه: microsatellite marker, dendogram, genetic distance, Nigerian indigenous goat,

چکیده مقاله :

This study was conducted to determine the genetic relationships among and population history of four Nigerian indigenous goat populations. A total of 200 goats from three breeds namely Sahel (60), Red Sokoto (60), West-African dwarf (60) and Kano Brown, a strain (20), were used for the study. Tissue samples were collected from the ear of animals using an Allflex® ear punch tissue sample collector, and aliquoted into plastic tubes containing the Allflex® tissue preservative. DNA extraction, amplification and sequencing were carried out at the International Livestock Research Institute (ILRI), Nairobi, Kenya. Genetic analysis of the DNA was carried out using 25 microsatellite markers proposed by Food and Agricultural Organization and International Society for Animal Genetics (FAO-ISAG). From the results of the study, the highest heterozygosity was observed in Kano Brown goats (0.68±0.04), which was followed by that of Red Sokoto goats (0.64±0.04) and that of West African dwarf goat (0.58±0.05) as the least. In the entire goat populations, low inbreeding was observed; mean Fis was 0.105 and Fit was 0.129. As expected, populations of Red Sokoto and Kano Brown showed higher genetic similarity as was seen in the genetic distance (0.022), confirming the notion that the Kano Brown is a strain from the Red Sokoto breed. Gene flow (Nm) played an important role in the genetic uniformity in populations of narrow geographical vicinity (14.868). The dendogram displayed a remarkable degree of consistency with the geographical origin of goats in this country. The information obtained in this study will aid in rational development, utilization and conservation of Nigerian indigenous goats.

چکیده انگلیسی:

این مطالعه با هدف تعیین رابطه ژنتیکی بین چهار جمعیت تاریخی بُز بومی نیجریه صورت گرفته است. 200 بُز از سه نژاد به نام­های ساحل (60)، سوکوتو قرمز (60)، کوتوله غرب آفریقا (60) و سویه قهوه­ای کانو (20) در این مطالعه به کار برده شدند. نمونه­های بافت از گوش حیوانات با استفاده از پانچ گوش آلفلکس جمع­آوری گردیده و در لوله­های پلاستیکی حاوی ماده نگهدارنده بافت آلفلکس قرار داده شدند. استخراج DNA تکثیر و توالی­یابی در موسسه بین­المللی تحقیقات حیوانات اهلی (ILRI) نایروبی کنیا انجام شد. آنالیز ژنتیکی DNA با استفاده از 25 نشانگر پیشنهاد شده مشترکاً توسط سازمان خواربار و کشاورزی ملل متحد (FAO) و انجمن بین­المللی ژنتیک حیوانی (ISAG) انجام شد. نتایج حاصل از این مطالعه نشان داد که بالاترین هتروزیگوتی به ترتیب در بُزهای قهوه­ای کانو (04/0±68/0) و سوکوتو قرمز (04/0±64/0) دیده شده و کمترین مقدار نیز در کوتوله غرب آفریقا (05/0±58/0) دیده می­شود. در کُل جمعیت بُزها، همخونی اندکی مشاهده گردیده و میانگین Fis و Fit به ترتیب 105/0 و 129/0 بود. همان­گونه که انتظار می­رفت، جمعیت­های سوکوتو قرمز و قهوه­ای کانو بالاتری قرابت ژنتیکی را داشتند (فاصله ژنتیکی برابر با 022/0). این موضوع تأیید می­کند که قهوه­ای کانو یک سویه از نژاد سوکوتو قرمز است. جریان ژنی (Nm) نقش مهمی در یکنواختی ژنتیکی در جمعیت­هایی که از نظر جغرافیایی همسایگی نزدیکی داشتند، ایفا می­کرد (868/14). دندروگرام نیز درجه مطابقت قابل توجهی با منشا جغرافیایی در این کشور داشت. اطلاعات حاصل از این مطالعه در توسعه، کاربرد و حفاظت از بُزهای بومی نیجریه مفید واقع می­شوند.

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