Morus nigra Fruit Extract Safeguards Frozen Thawed Bovine Sperm Parameters

محورهای موضوعی : Camel

S. Suleman ¹ , M.A. Kanwal ² , R. Ali ³ , N. Kanwal ⁴ , S. Yasmeen ⁵ , M.Y. Mehmood ⁶ , S. Siddique ⁷ , S.N. Ahmad ⁸ , A. Younis ⁹ , I. Inayat ¹⁰ , K. Raees Ahmad ¹¹

1 - Government Associate College (for Women), Mochh, Mianwali, Punjab, Pakistan
2 - Department of Zoology, University of Sargodha, Sargodha, Punjab, Pakistan
3 - Department of Zoology, University of Sargodha, Sargodha, Punjab, Pakistan
4 - Department of Zoology, University of Sargodha, Sargodha, Punjab, Pakistan
5 - Government College University, Faisalabad, Pakistan
6 - Riphah College of Veterinary Sciences, Lahore, Pakistan
7 - Department of Zoology, University of Sargodha, Sargodha, Punjab, Pakistan
8 - Department of Zoology, University of Chakwal, Chakwal, Pakistan
9 - Department of Zoology, University of Sargodha, Sargodha, Punjab, Pakistan
10 - Department of Zoology, University of Sargodha, Sargodha, Punjab, Pakistan
11 - Government Ambala Muslim Graduate College, Sargodha, Pakistan

تاریخ دریافت : 1401/06/24 تاریخ پذیرش : 1401/09/17 تاریخ انتشار : 1402/03/11

کلید واژه: semen quality, Oxidative stress, cryopreservation, cryo-injuries, <i>Morus nigra</i>,

چکیده مقاله :

This study explored the effect of Morus nigra fruit extract (MFE) on microanatomical and physiological parameters of cryopreserved bovine sperms. Three ejaculates were collected on weekly basis, from five fertile bulls. Each semen sample was mixed with dilution medium (DM) (1:2 v/v), centrifuged (32 g) for 10 minutes to pore away the upper-half of the mixture. The remaining material was then extended (1:4 ratios) with standard cryopreservation extender (SCE). Three aliquots (0.3 mL) from this extended sample were further extended with 0.7 mL pure SCE (control group), 0.7 mL SCE containing 3% MFE (MFE-3 group) and 0.7 mL SCE containing 6% MFE (MFE-6 group) respectively to attain final dilutions (25 times dilution of fresh semen ejaculate, containing approximately 1836.5 ± 85.23 million sperms per mL). From each final dilution (0.1 mL) sample was directly analyzed for semen quality parameters (SQPs) and rest of the material was placed in liquid nitrogen for 24 hrs for post thaw study of the SQPs and in-vitro fertilizability. Results revealed substantial improvement in sperm membrane integrity, motility, and fertilizability in MFE-3 and MFE-6 against SCE group. Likewise, significantly high mean percent number of progressively motile sperms and sperms showing 20 µ/sec or above velocity both before and after cryopreservation, were observed in MFE-6 and MFE-3 groups as compared to SCE. These findings show protective effects of MFE for bovine spermatozoa against cryoinjuries and the post thawed oxidative stress.

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