A Profile of Single Nucleotide Polymorphisms in Fecundity Genes Among Iranian Sheep Breeds by Using Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP) Method
محورهای موضوعی : Camelپ. پتکی 1 , س.ض. میرحسینی 2 , ف. افراز 3 , س.م.ف. وحیدی 4
1 - Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), North Branch, Rasht, Iran
2 - Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
3 - Department of Animal Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), North Branch, Rasht, Iran
4 - Department of Animal Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), North Branch, Rasht, Iran
کلید واژه: PCR-RFLP, Sheep, GDF9, SNP, BMP15, BMPR1B,
چکیده مقاله :
Ovulation rate and litter size are traits of fecundity and have a very remarkable economic value that leads to an increase in the production of meat, wool and milk. Therefore, exploring on the candidate and functional genes for these traits is very important. Growth differentiation factor 9 (GDF9), bone morphogenetic protein 15 (BMP15) and bone morphogenetic protein receptor-1B (BMPR1B) are the most important of these genes containing single nucleotide polymorphisms (SNPs) markers that are associated with high fertility in sheeps. The aim of this study was to determine the allelic and genotypic frequencies of seven of these SNPs including; G1, G4, G7 and G8 in GDF9 gene, B2 and B4 in BMP15 gene and FecB in BMPR1B gene among 532 sheeps from eleven of Iranian native breeds using polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method. For each mutation region, polymerase chain reactions and digestion reactions with specific endonuclease enzymes, were performed. The results showed that G1 mutation presents among ten breeds except Zandi, G4 mutation in all breeds, and the FecB mutation in two breeds; Kalakuei and Zandi. The G7, G8, B2 and B4 mutations were not observed in any of breeds, and all sheeps were homozygote wild type for these four positions.
نرخ تخمک گذاری و میزان بره زایی، از ویژگیهای باروری بوده که ارزش اقتصادی فراوانی در گوسفندان داشته و به افزایش تولید گوشت، پشم و شیر می انجامد. از این رو کاوش بر روی ژن های کاندید و کارساز بر این ویژگی ها جایگاه ارزندهای دارد. سه ژن GDF9، BMP15 و BMPR1B از مهمترین این ژن ها هستند که دارای چندین جهش تکنوکلئوتیدی (SNPs) مرتبط با باروری بالا در گوسفندان می باشند. در این پژوهش، به برآورد فراوانی ژنی و ژنوتیپی هفت جهش تک نوکلئوتیدی؛ جهش های G1، G4، G7 و G8 در ژن GDF9، جهش های B2 و B4 در ژن BMP15 و جهش FecB در ژن BMPR1B در میان 532 گوسفند از 11 نژاد گوسفندان بومی ایران به روش PCR-RFLP پرداخته شد. به ازای هر یک از مناطق جهشی، واکنش های زنجیرهای پلیمراز و نیز گوارش آنزیمی با آنزیم های برشی ویژه انجام شد. دستاوردها نشان داد که جهش G1 در ژن GDF9 در میان 10 نژاد بجز نژاد زندی، جهش G4 در همین ژن در میان هر 11 نژاد و جهش FecB در ژن BMPR1B تنها در میان دو نژاد کلکویی و زندی وجود دارد. جهش های G7 و G8 در ژن GDF9 و جهش های B2 و B4 در ژن BMP15 در هیچ یک از این 11 نژاد دیده نشدند و همگی گوسفندان برای این چهار جایگاه، هموزیگوت غالب بودند.
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