نمایی از چندشکلی های تک نوکلئوتیدی در ژن های باروری در میان نژادهای گوسفند ایران با بکارگیری روش PCR-RFLP

Subject Areas : Camel

پ. پتکی ¹ , س.ض. میرحسینی ² , ف. افراز ³ , س.م.ف. وحیدی ⁴

1 - Department of Genomics, Agricultural Biotechnology Research Institute of Iran (ABRII), North Branch, Rasht, Iran
2 - Department of Animal Science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
3 - Department of Animal Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), North Branch, Rasht, Iran
4 - Department of Animal Biotechnology, Agricultural Biotechnology Research Institute of Iran (ABRII), North Branch, Rasht, Iran

Received: 2019-01-14 Accepted : 2019-08-15 Published : 2020-06-01

Keywords: PCR-RFLP, GDF9, گوسفند, BMP15, BMPR1B, جهش تک نوکلئوتیدی,

Abstract :

نرخ تخمک­ گذاری و میزان بره ­زایی، از ویژگی­های باروری بوده که ارزش اقتصادی فراوانی در گوسفندان داشته و به افزایش تولید گوشت، پشم و شیر می­ انجامد. از این رو کاوش بر روی ژن­ های کاندید و کارساز بر این ویژگی­ ها جایگاه ارزنده­ای دارد. سه ژن­ GDF9، BMP15 و BMPR1B از مهم­ترین این ژن­ ها هستند که دارای چندین جهش تک­نوکلئوتیدی (SNPs) مرتبط با باروری بالا در گوسفندان می ­باشند. در این پژوهش، به برآورد فراوانی ژنی و ژنوتیپی هفت جهش تک نوکلئوتیدی؛ جهش­ های G1، G4، G7 و G8 در ژن GDF9، جهش های B2 و B4 در ژن  BMP15 و جهش FecB در ژن BMPR1B در میان 532 گوسفند از 11 نژاد گوسفندان بومی ایران به روش PCR-RFLP پرداخته شد. به ازای هر یک از مناطق جهشی، واکنش­ های زنجیره­ای پلی­مراز و نیز گوارش آنزیمی با آنزیم­ های برشی ویژه انجام شد. دستاوردها نشان داد که جهش G1 در ژن GDF9 در میان 10 نژاد بجز نژاد زندی، جهش G4 در همین ژن در میان هر 11 نژاد و جهش FecB در ژن BMPR1B تنها در میان دو نژاد کلکویی و زندی وجود دارد. جهش ­های G7 و G8 در ژن GDF9 و جهش­ های B2 و B4 در ژن  BMP15 در هیچ یک از این 11 نژاد دیده نشدند و همگی گوسفندان برای این چهار جایگاه، هموزیگوت غالب بودند.

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