Motility, Viability, and Abnormality of the Spermatozoa of Bali Bull with Andromed® and Egg Yolk-Tris Diluents Stored at 4 °C

محورهای موضوعی : Camel

ر. سوهاردی ¹ , م. مگاواتی ² , ف. اردهانی ³ , پ. سامپون ⁴ , س. ووتیسوتیمتو ⁵

1 - School of Agricultural Technology, Walailak University, Nakhon Si Thammarat, Thailand|Department of Animal Science, Faculty of Agriculture, Mulawarman University, Samarinda, East Kalimantan, Indonesia
2 - Vocational High School 1 Penajam Paser Utara, Majoring in Animal Husbandry, Sepaku, East Kalimantan, Indonesia
3 - Department of Animal Science, Faculty of Agriculture, Mulawarman University, Samarinda, East Kalimantan, Indonesia
4 - Food Technology and Innovation Research Center of Excellence, School of Agricultural Technology, Walailak University, Nakhon Si Thammarat 80161, Thailand
5 - School of Agricultural Technology, Walailak University, Nakhon Si Thammarat, Thailand

تاریخ دریافت : 1398/03/10 تاریخ پذیرش : 1398/04/24 تاریخ انتشار : 1399/03/12

کلید واژه: viability, Abnormality, motility, Andromed®, Bali bull semen, egg yolk-tris,

چکیده مقاله :

Bali bull has great potential to be developed as a national meat source in Indonesia which can address concerns of meat importation. Bali bull farming only requires small holding system, has high fertility and low calf mortality as its advantages and is a growing enterprise by way of artificial insemination (AI) with semen collected from phenotypically superior bull sires. However, efficient means of preserving Bali bull semen quality using diluents still requires exploration at a national level. The purpose of this study was to determine which of the selected diluents will best preserve the quality of local Bali bull semen over a period of five days at 4 ˚C. Andromed® and egg yolk-tris diluents were compared in preserving quality of semen collected from seven-year old bulls with 70% minimum motility. Spermatozoa motility, viability, and abnormality were observed and compared. This research used factorial complete randomized design with two factors in which each factor has 2 levels and 5 levels with three-times treatment. Results of the study showed Bali bull spermatozoa demonstrated 51.66% motility, 59.13% viability, and only 22.68% abnormality until day five in egg yolk-tris diluent. In comparison, Andromed® diluent demonstrated 23.33% motility, 45.59% viability, and 23.15% abnormality under similar conditions. To conclude, egg yolk-tris is a superior diluent when compared to Andromed® in preserving local Bali bull semen quality by better maintaining spermatozoa motility, viability and lower percentages of abnormalities when stored for five days at 4 ˚C. This data provides useful information for bull farmers, in particular Bali bull farmers in Indonesia in the practice of AI to consider carefully the choice of diluent relative to storage time and temperature in improving cattle farming practices for increased meat productivity in the region.

چکیده انگلیسی:

گاو نر Bali پتانسیل بالایی برای توسعه به­ عنوان یک منبع گوشت ملی در اندونزی دارد که می‌تواند نگرانی‌های مربوط به واردات گوشت را برطرف کند. پرورش گاو نر Bali تنها نیازمند سیستم‌های نگهداری کوچک است، دارای باروری بالا و مرگ­و­میر گوساله پایین به ­عنوان مزایای آن یک سرمایه‌گذاری در حال رشد از طریق تلقیح مصنوعی (AI) با منی جمع‌آوری شده گاوهای نر برتر فنوتیپی است. با این حال، ابزار کارآمد برای حفظ کیفیت منی گاو نر Bali با استفاده از رقیق­کننده‌ها هنوز هم نیاز به اکتشاف در سطح ملی دارد. هدف از این پژوهش تعیین اینکه کدام یک از رقیق­کننده‌های انتخاب شده به بهترین وجه کیفیت مایع منی گاو نر Bali محلی را برای مدت پنج روز در دمای 4 درجه سلسیوس حفظ می‌کند. رقیق­کننده‌های Andromed و تریس-زرده تخم ‌مرغ در حفظ کیفیت منی جمع آوری شده از گاوهای هفت ساله با حداقل تحرک 70 درصد مقایسه شدند. تحرک، زنده‌مانی، و نابهنجاری اسپرماتوزوآ مشاهده و مقایسه شدند. این تحقیق از طرح فاکتوریل کاملا تصادفی با دو فاکتور که هر فاکتور دارای دو سطح و پنج سطح با سه سطح زمانی استفاده کرده است. نتایج این مطالعه نشان داد اسپرماتوزوآ گاو نر Bali 51.66 درصد تحرک، 59.13 درصد زنده‌مانی، و تنها 22.68 درصد نابهنجاری تا روز پنج در رقیق­کننده تریس-زرده تخم ‌مرغ نشان داده است. در مقایسه، رقیق­کننده Andromed 33/23 درصد تحرک، 59/45 درصد زنده‌مانی، و 23.15 درصد نابهنجاری تحت شرایط مشابه نشان داده است. برای نتیجه­گیری، تریس-زرده تخم ‌مرغ یک رقیق کننده قوی هنگام مقایسه با Andromed در نگهداری کیفیت اسپرم گاوهای بومی Bali با بهترین حفظ تحرک، زنده‌مانی اسپرماتوزوآ و کمترین درصد نابهنجاری هنگام ذخیره برای 5 روز در دمای 4 درجه سلسیوس است. این داده‌ها اطلاعات مفیدی برای پرورش دهندگان گاو نر فراهم می‌کند، به ویژه پرورش دهندگان گاو نر Bali در اندونزی در عمل تلقیح مصنوعی برای در نظر گرفتن دقیق انتخاب رقیق‌کننده نسبت به زمان نگهداری و دما در بهبود روش‌های پرورش گاو برای افزایش بهره‌وری گوشت در این منطقه می‌باشد.

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