Effect of Oocyte Maturation Period on Developmental Rate and Sex ratio Distribution of in vitro Produced Bovine Embryos
محورهای موضوعی : CamelH. Nazari 1 , N. Esfandabadi 2 , A. Shirazi 3 , A.M. Ahadi 4 , E. Ahmadi 5 , A. Safdarinejad 6
1 - Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
2 - Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran|Department of Clinical Science, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
3 - Reproductive Biotechnology Research Center, Avicenna Research Institute, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
4 - Department of Genetics, Faculty of Science, Shahrekord University, Shahrekord, Iran
5 - Research Institute of Animal Embryo Technology, Shahrekord University, Shahrekord, Iran
6 - Department of Clinical Science, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran
کلید واژه: Embryo, sex ratio, bovine, oocyte, <i>in vitro</i> maturation,
چکیده مقاله :
It has been suggested that the maturation status of the oocytes at the time of fertilization influences the sex ratio of resulting embryos produced in vitro. Therefore, the duration of the oocyte in vitro maturation is likely a simple method that leads to in vitro production of embryos with the desired sex. For this purpose, the current study was conducted to investigate the effect of in vitro maturation culture period of bovine oocytes on the sex ratio of resulting blastocysts. The abattoir ovary-derived oocytes were cultured for various duration of in vitro maturation (16 h, 20 h, 24 h, 28 h, and 36 h). After in vitro fertilization and culture of mature oocytes, nested multiplex polymerase chain reaction (PCR) with AMELX and SRY primers was used to determine the sex of blastocysts. The results of this study showed that the cleavage and blastocyst rates were increased by prolongation of in vitro maturation (IVM) duration from 16 hours to 24 hours and deceased from 24 hours to 36 hours (P<0.05). The sex ratio of male embryos in aged oocytes (36 hours IVM) was significantly higher than in other groups (P<0.05). In conclusion, the maturation duration of bovine COCs influences the sex distribution of embryos in the blastocyst stage appearing on day 8, and the male embryos were recovered more than female counterparts by increasing the oocytes culture period.
Agung B., Otoi T., Wongsrikeao P., Taniguchi M., Shimizu R., Watari H. and Nagai T. (2006). Effect of maturation culture period of oocytes on the sex ratio of in vitro fertilized bovine embryos. J. Reprod. Dev. 52(1), 123-127.
Avery B., Schmidt M. and Greve T. (1989). Sex determination of bovine embryos based on embryonic cleavage rates. Acta Vet. Scandinavica. 30(2), 147-153.
Bredbacka K. and Bredbacka P.J.R. (1996). Glucose controls sex-related growth rate differences of bovine embryos produced in vitro. J. Reprod. Fertil. 106(2), 169-172.
Dallemagne M., Ghys E., De Schrevel C., Mwema A., De Troy D., Rasse C. and Donnay I. (2018). Oxidative stress differentially impacts male and female bovine embryos depending on the culture medium and the stress condition. Theriogenology. 117, 49-56.
Dominko T. and First N.L. (1997). Relationship between the maturational state of oocytes at the time of insemination and sex ratio of subsequent early bovine embryos. Theriogenology. 47(5), 1041-1050.
Gutierrez-Adan A., Lonergan P., Rizos D., Ward F., Boland M., Pintado B. and De La Fuente J.J.T. (2001). Effect of the in vitro culture system on the kinetics of blastocyst development and sex ratio of bovine embryos. Theriogenology. 55(5), 1117-1126.
Gutiérrez-Adán A., Pérez G., Granados J., Garde J.J., Pérez-Guzmán M., Pintado B. and De La Fuente J. (1999). Relationship between sex ratio and time of insemination according to both time of ovulation and maturational state of oocyte. Zygote. 7(1), 37-43.
Kochhar H.S., Kochhar K.P., Basrur P.K. and King W.A.J.A.R.S. (2003). Influence of the duration of gamete interaction on cleavage, growth rate and sex distribution of in vitro produced bovine embryos. Anim. Reprod. Sci. 77(1), 33-49.
Macaulay A.D., Hamilton C.K., King W.A. and Bartlewski P.M. (2013). Influence of physiological concentrations of androgens on the developmental competence and sex ratio of in vitro produced bovine embryos. Reprod. Biol. 13(1), 41-50.
Marquant-leGuienne B., Nibart M., Guyader C., Kohen G., Esposito L., Thuard J. and Thibier M.J.T. (1992). DNA probe sexing of young in vitro fertilized bovine embryos. Theriogenology. 37, 253-262.
Martinez F., Kaabi M., Martinez-Pastor F., Alvarez M., Anel E., Boixo J.C., de Paz P. and Anel L. (2004). Effect of the interval between estrus onset and artificial insemination on sex ratio and fertility in cattle: A field study. Theriogenology. 62(7), 1264-1270.
Miao Y.L., Kikuchi K., Sun Q.Y. and Schatten H. (2009). Oocyte aging: cellular and molecular changes, developmental potential and reversal possibility. Hum. Reprod. Update. 15(5), 573-585.
Peippo J., Kurkilahti M. and Bredbacka P.J.Z. (2001). Developmental kinetics of in vitro produced bovine embryos: The effect of sex, glucose and exposure to time-lapse environment. Zygote. 9(2), 105-111.
Rahman M.S. and Pang M.G. (2020). New biological insights on X and Y chromosome-bearing spermatozoa. Front. Cell Dev. Biol. 7, 388-396.
Rheingantz M., Pegoraro L., Dellagostin O., Pimentel A., Bernardi M. and Deschamps J.J.A.R. (2018). The sex ratio of in vitro produced bovine embryos is affected by the method of sperm preparation. Anim. Reprod. 3(4), 423-430.
Rizos D., Bermejo-Alvarez P., Gutierrez-Adan A. and Lonergan P. (2008). Effect of duration of oocyte maturation on the kinetics of cleavage, embryo yield and sex ratio in cattle. Reprod. Fertil. Dev. 20(6), 734-740.
Rorie R.J.T. (1999). Effect of timing of artificial insemination on sex ratio. Theriogenology. 52(8), 1273-1280.
Steele H., Makri D., Maalouf W., Reese S. and Kölle S.J.S.R. (2020). Bovine sperm sexing alters sperm morphokinetics and subsequent early embryonic development. Sci. Rep. 10(1), 1-13.
Tarín J.J., Pérez-Albalá S., Pérez-Hoyos S. and Cano A.J.B.O.R. (2002). Postovulatory aging of oocytes decreases reproductive fitness and longevity of offspring. Biol. Reprod. 66(2), 495-499.
Wehner G.R., Wood C., Tague A., Barker D. and Hubert H. (1997). Efficiency of the OVATEC unit for estrus detection and calf sex control in beef cows. Anim. Reprod. Sci. 46(1), 27-34.
Wolf C., Brass K., Rubin M., Pozzobon S., Mozzaquatro F. and De La Corte F.J.A.R. (2018). The effect of sperm selection by Percoll or swim-up on the sex ratio of in vitro produced bovine embryos. Anim. Reprod. 5(3), 110-115.
