A Research on Association between SCD1 and OLR1 Genes and Milk Production Traits in Iranian Holstein Dairy Cattle
محورهای موضوعی : Camel
م. حسین پور مشهدی
1
(
Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
)
کلید واژه: Holstein, gene, <, i>, OLR1<, /i>, , <, i>, SCD1<, /i>, ,
چکیده مقاله :
The present study was carried out to investigate the association of C/T single nucleotide polymorphism(SNP)in exon 5 of stearoyl-CoA desaturase 1 (SCD1) gene and A/C SNP in the 3' untranslated region of oxidized low density lipoprotein receptor 1 (OLR1)gene with milk production traits in Iranian Holstein dairy Cattle. The blood samples of 153 (for OLR1) and 308 (for SCD1) dairy cattle from three different farms were used for genotyping. A 146 bp fragment of 3'UTR of OLR1 gene and a 400 bp fragment of exon 5 of SCD1 gene were amplified by standard PCR. Single nucleotide polymorphism of OLR1 and SCD1 gene was determined by polymerase chain reaction single-restriction fragment length polymorphism (PCR-RFLP) technique. The association between genotypes of OLR1 and SCD1 genes with milk production traits was studied by general linear models (GLM) procedure of SAS package and Duncan test was used for comparing means of traits. The frequency of AA, AV and VV genotype of SCD1 gene were 0.60, 0.32 and 0.08 respectively. The frequency of alleles A and V were 0.76 and 0.24. The genotype frequencies of AA, AC and CC in OLR1 gene were 0.22, 0.50 and 0.28 respectively. The frequency of allele A and C was 0.47 and 0.53. Thus, this population was in Hardy-Weinberg equilibrium for OLR1 but not for SCD1. The means of fat percentage for SCD1 genotypes were 3.43% and 3.33 for VV and AA respectively (P<0.05). The means of OLR1 genotypes were 8273 kg (CC), 8344 kg (AC) and 7178 kg (AA) for milk yield; 276.3 kg (CC), 277.6 kg (AC) and 239.7 kg (AA) for fat yield and for protein yield were 286.7 kg (CC), 290.5 kg (AC) and 253 kg (AA) (P<0.05). The results revealed these two SNP are appropriate for marker assisted selection.
تحقیق حاضر به منظور بررسی ارتباط بین چند‌شکلی تک نوکلئوتیدی C/T در اگزون 5 از ژن SCD1 و چند‌شکلی تک نوکلئوتیدی A/C در ناحیه غیر قابل ترجمه ‘3 ژن OLR1 با صفات تولید شیر در گاوهای شیری هلشتاین ایرانی انجام شد. خونگیری برای تعیین ژنوتیپ از تعداد 153 رأس (برای OLR1) و از تعداد 308 راس (برای SCD1) از سه گله مختلف انجام شد. قطعه bp 146 از ناحیه غیر قابل ترجمه ‘3 ژن OLR1 و قطعه bp 400 از اگزون 5 از ژن SCD1 با روش PCR استاندارد تکثیر شد. چند‌شکلی‌های تک نوکلئوتیدی ژن‌های OLR1 و SCD1 توسط روش PCR-RFLP تعیین شد. ارتباط بین ژنوتیپ ژن‌های OLR1 و SCD1 با صفات تولید شیر توسط روش مدل خطی عمومی (GLM) نرم افزار SAS و میانگین صفات با روش دانکن مقایسه شد. فراوانی ژنوتیپ‌های AA، AV و VV ژن SCD1 به ترتیب 6/0، 32/0 و 08/0 برآورد شد. فراوانی آلل‌های A و V به ترتیب 76/0 و 24/0 برآورد شد. فراوانی ژنوتیپ‌های AA، AC و CC ژن OLR1 به ترتیب 22/0، 5/0 و 28/0 برآورد شد. فراوانی آلل‌های A و C به ترتیب 47/0 و 53/0 برآورد شد. این جوامع برای ژن OLR1 در تعادل هاردی واینبرگ بود اما برای ژن SCD1 در تعادل نبود. میانگین درصد چربی برای ژنوتیپ‌های VV و AA در ژن SCD1 به ترتیب 43/3 درصد و 33/3 درصد بود (05/0>P). در ژن OLR1 میانگین مقدار شیر 8273، 8344 و 7178 کیلوگرم به ترتیب برای ژنوتیپ‌های CC، AC و AA، میانگین مقدار چربی 3/276، 6/277 و 7/239 کیلوگرم به ترتیب برای ژنوتیپ‌های CC، AC و AA و برای میانگین مقدار پروتئین 7/286، 5/290 و 253 کیلوگرم به ترتیب برای ژنوتیپ‌های CC، AC و AA برآورد شد (05/0>P). نتایج نشان داد که این دو SNP برای انتخاب به کمک نشانگر مناسب می‌باشند
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