A Research on Association between SCD1 and OLR1 Genes and Milk Production Traits in Iranian Holstein Dairy Cattle
الموضوعات :
م. حسین پور مشهدی
1
(Department of Animal Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran)
الکلمات المفتاحية: Holstein, gene, <, i>, OLR1<, /i>, , <, i>, SCD1<, /i>, ,
ملخص المقالة :
The present study was carried out to investigate the association of C/T single nucleotide polymorphism(SNP)in exon 5 of stearoyl-CoA desaturase 1 (SCD1) gene and A/C SNP in the 3' untranslated region of oxidized low density lipoprotein receptor 1 (OLR1)gene with milk production traits in Iranian Holstein dairy Cattle. The blood samples of 153 (for OLR1) and 308 (for SCD1) dairy cattle from three different farms were used for genotyping. A 146 bp fragment of 3'UTR of OLR1 gene and a 400 bp fragment of exon 5 of SCD1 gene were amplified by standard PCR. Single nucleotide polymorphism of OLR1 and SCD1 gene was determined by polymerase chain reaction single-restriction fragment length polymorphism (PCR-RFLP) technique. The association between genotypes of OLR1 and SCD1 genes with milk production traits was studied by general linear models (GLM) procedure of SAS package and Duncan test was used for comparing means of traits. The frequency of AA, AV and VV genotype of SCD1 gene were 0.60, 0.32 and 0.08 respectively. The frequency of alleles A and V were 0.76 and 0.24. The genotype frequencies of AA, AC and CC in OLR1 gene were 0.22, 0.50 and 0.28 respectively. The frequency of allele A and C was 0.47 and 0.53. Thus, this population was in Hardy-Weinberg equilibrium for OLR1 but not for SCD1. The means of fat percentage for SCD1 genotypes were 3.43% and 3.33 for VV and AA respectively (P<0.05). The means of OLR1 genotypes were 8273 kg (CC), 8344 kg (AC) and 7178 kg (AA) for milk yield; 276.3 kg (CC), 277.6 kg (AC) and 239.7 kg (AA) for fat yield and for protein yield were 286.7 kg (CC), 290.5 kg (AC) and 253 kg (AA) (P<0.05). The results revealed these two SNP are appropriate for marker assisted selection.
Ashwell M.S., Heyen D.W., Sonstegard T.S., Van Tassell C.P., Da Y., VanRaden P.M., Ron M., Weller J.I. and Lewin H.A. (2004). Detection of quantitative trait loci affecting milk production, health, and reproductive traits in Holstein cattle. J. Dairy Sci. 87, 468-475.
Campbell E.M.G., Gallagher D.S., Davis S.K., Taylor J.F. and Smith S.B. (2001). Mapping of the bovine stearoyl coenzyme A desaturase (SCD) gene to BTA26. J. Anim. Sci. 79, 1954-1955.
Cecchinato A., Chessa S., Ribeca C., Cipolat-Gotet C., Bobbo T., Casellas J. and Bittante G. (2015). Genetic variation and effects of candidate-gene polymorphisms on coagulation properties, curd firmness modeling and acidity in milk from Brown Swiss cows. Animal. 9(7), 1104-1112.
Clark L.A., Thomson J.M., Moore S.S. and Oba M. (2010). The effect of Ala293Val single nucleotide polymorphism in the stearoyl-CoA desaturase gene on conjugated linoleic acid concentration in milk fat of dairy cows. Canadian J. Anim. Sci. 90, 575-584.
De Koning D.J., Schulmant N.F., Elo K., Moisio S., Kinos R., Vilkki J. and Maki-Tanila A. (2001). Mapping of multiple quantitative trait loci by simple regression in half-sib designs. J. Anim. Sci. 79, 616-622.
Heyen D.W., Weller J.I., Ron M., Band M., Beever J.E., Feldmesser E., Da Y., Wiggans G.R., VanRaden P.M. and Lewin H.A. (1999). A genome scans for QTL influencing milk production and health traits in dairy cattle. Physiol. Genomics. 1, 165-175.
Hosseinpour Mashhadi H., Nassiri M.R., Emam Jome Kashan N. and Vaez Torshizi R. (2013). Associationbetween DGAT1 genotype and breeding value of milk production traits in Iranian Holstein bulls. Iranian J.Appl. Anim. Sci. 3, 811-815.
Imanishi T., Hano T., Sawamura T., Takarada S. and Nishio I. (2002). Oxidized low density lipoprotein potentiation of Fas-induced apoptosis through lectin-like oxidized-low density lipoprotein receptor-1 in human umbilical vascular endothelial cells. Circ. J. 66, 1060-1064.
Kgwatalala P.M., Ibeagha-Awemu E.M., Mustafa A.F. and Zhao X. (2009). Influence of stearoyl-coenzyme a desaturase 1genotype and stage of lactation on fatty acid composition of Canadian Jersey cows. J. Dairy Sci. 92, 1220-1228.
Khatib H., Leonard S.D., Schutzkus V., Luo W. and Chang Y.M. (2006). Association of the OLR1 gene with milk composition in Holstein dairy cattle. J. Dairy Sci. 89, 1753-1760.
Khatib H., Rosa G.J., Weigel K., Schiavini F., Santus E. and Bagnato A. (2007). Additional support for an association between OLR1 and milk fat traits in cattle. Anim. Genet. 38, 308-310.
Komisarek J. and Dorynek Z. (2009). Effect of ABCG2, PPARGC1A, OLR1 and SCD1 gene polymorphism on estimated breeding values for functional and production traits in Polish Holstein-Friesian bulls. J. Appl. Genet. 50, 125-132.
Mehta J.L. and Li D. (2002). Identification, regulation and function of a novel lectin–like oxidized low–density lipoprotein receptor. J. Am. Coll. Cardiol. 39, 1429-1435.
Mehta J.L. and Li D. (1998). Identification and autoregulation of receptor for OX-LDL in cultured human coronary artery endothelial cells. Biochem. Biophys. Res. Commun. 248, 511-514.
Mele M., Conte G., Castiglioni B., Chessa S., Macciotta N.P., Serra A., Buccioni A., Pagnacco G. and Secchiari P. (2007). Stearoyl-CoA desaturase gene polymorphism and milk fatty acid composition in Italian Holsteins. J. Dairy Sci. 90, 4458-4465.
Moioli B., Contarini G., Avalli A., Catillo G., Orru L., De Matteis G., Masoero G. and Napolitano F. (2007). Short communication: effect of stearoyl-coenzyme a desaturase polymorphism on fatty acid composition of milk. J. Dairy Sci. 90, 3553-3558.
Olsen H.G., Gomez-Raya L., Vage D.I., Olsaker I., Klungland H., Svendsen M., Adnoy T., Sabry A., Klemetsdal G., Schulman N., Kramer W., Thaller G., Ronningen K. and Lien S. (2002). A genome scan for quantitative trait loci affecting milk production in Norwegian dairy cattle. J. Dairy Sci. 85, 3124-3130.
Rodriguez-Zas S.L., Southey B.R., Heyen D.W. and Lewin H.A. (2002). Interval and composite interval mapping of somatic cell score, yield, and components of milk in dairy cattle. J. Dairy Sci. 85, 3081-3091.
Rychtarova J., Sztankoova Z., Kyselova J., Zink V., Stipkova M., Vacek M. and Stolc L. (2014). Effect of DGAT1, BTN1A1, OLR1 and STAT1 genes on milk production and reproduction traits in the Czech Fleckvieh breed. Czech J. Anim. Sci. 59 (2), 45-53.
SAS Institute. (2004). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Sawamura T., Kume N., Aoyama T., Moriwaki H., Hoshikawa H., Aiba Y., Tanaka T., Miwa S., Katsura Y., Kita T. and Masaki T. (1997). An endothelial receptor for oxidized low-density lipoprotein. Nature. 386, 73-77.
Schennink A., Heck J.M., Bovenhuis H., Visker M.H., Van Valenberg H.J. and Van Arendonk J.A. (2008). Milk fatty acid unsaturation: genetic parameters and effects of stearoyl-CoA desaturase (SCD1) and acyl-CoA: diacylglycerol acyltransferase 1 (DGAT1). J. Dairy Sci. 91, 2135-2143.
Schennink A., Bovenhuis H., Le´on-Kloosterziel K.M., van Arendonk J.A.M. and Visker M.H.P.W. (2009). Effect of polymorphisms in the FASN, OLR1, PPARGC1A, PRL and STAT5A genes on bovine milk-fat composition. Anim. Genet. 40, 909-916.
Soltani-Ghombavan M., Ansari-Mahyari S., Ghorbani G.R. and Edriss M.A. (2013). Association of a polymorphism in 3' untranslated region of OLR1 gene with milk fat and protein in dairy cows. Arch. Tierz. 56, 32-40.
Taniguchi M., Utsugi T., Oyama K., Mannen H., Kobayashi M., Tanabe Y., Ogino A. and Tsuji S. (2004). Genotype of stearoyl-CoA desaturase is associated with fatty acid composition in Japanese Black cattle. Mamm. Genome. 14, 142-148.
Viitala S.M., Schulman N.F., de Koning D.J., Elo K., Kinos R., Virta A., Virta J., Maki-Tanila A. and Vilkki J.H. (2003). Quantitative trait loci affecting milk production traits in Finnish Ayrshire dairy cattle. J. Dairy Sci. 86, 1828-1836.
Wang Q., Hulzebosch A. and Bovenhuis H. (2016). Genetic and environmental variation in bovine milk infrared spectra. J. Dairy Sci. 99(8), 6793-6803.
Yeh F.C., Yang R.C. and Boyle T. (1999). POPGENE. Microsoft Windows-Based Freeware for Population Genetic Analysis. Release 1.31. Univ Alberta, Edmonton, Canada.
