Association of IGF-I Gene Polymorphisms with Carcass Traits in Iranian Mehraban Sheep Using SSCP Analysis
محورهای موضوعی : CamelS. Behzadi 1 , S.R. Miraei-Ashtiani 2 , M. Sadeghi 3 , P. Zamani 4 , R. Abdoli 5
1 - Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran
2 - Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran
3 - Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran
4 - Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
5 - Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
کلید واژه: carcass traits, single nucleotide polymorphism, insulin-like growth factor I, Mehraban sheep, single-strand conformational polymorphism,
چکیده مقاله :
Molecular genetics selection on individual genes is a promising method to genetically improve economically important traits in livestock. The insulin like growth factor-I (IGF-I) gene may play important roles in growth of multiple tissues, including muscle cells, cartilage and bone. The objectives of the present study were the estimate the haplotype frequencies of the IGF-I gene polymorphisms in Iranian Mehraban sheep and to determine associations between polymorphisms and carcass traits in 439 individuals. Polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) and DNA sequencing methods were employed in screening for genetic variation. Two single nucleotide polymorphisms (C114G and G116A) and two amino acid exchange (S5T and G6S) withthree patterns were found in IGF-I gene fragment. Associations of IGF-I patterns with blood cholesterol and blood triglycerides were significant (P<0.05) and tended to be significant (P<0.1) for dorsal fat thick and carcass weight. The other studied traits were not significantly affected by different patterns of IGF-I. This finding indicates that IGF-I polymorphisms may be used as DNA markers for selection in the breeding process of Mehraban sheep.
انتخاب به کمک ژنتیک مولکولی با استفاده از ژنهای اختصاصی یک روش پیشنهاد شده برای بهبود ژنتیکی صفات مهم اقتصادی در دام‌ها میباشد. ژن IGF-I ممکن است نقشهای مهمی در رشد بافتهای متفاوت شامل سلولهای عضلانی، غضروف و استخوان بازی کند. اهداف مطالعه حاضر برآورد فراوانیهای هاپلوتایپی چندشکلیهای ژن IGF-I در گوسفندان نژاد مهربان و تعیین ارتباط بین این چندشکلیها و صفات لاشه در 439 حیوان بودند. واکنش زنجیرهای پلیمراز با استفاده از روشهای چندشکلی آرایش فضایی رشته‌های منفرد (PCR-SSCP) و توالی یابی برای شناسایی تفاوتهای ژنتیکی بکار گرفته شدند. دو چندشکلی تک نوکلئوتیدی (C114G و G116A) و دو تغییر آمینو اسیدی (S5T و G6S) با سه الگوی متفاوت در قطعه تکثیر شده از ژن IGF-I شناسایی شدند. ارتباط الگوهای ژن IGF-I با کلسترول و تریگلیسرید خون معنیدار (05/0P<) و برای ضخامت چربی پشتی و وزن لاشه متمایل به معنیدار (1/0P<) بود. دیگر صفات مطالعه شده تحت تأثیر معنی‌داری به وسیله الگوهای متفاوت ژن IGF-I قرار نگرفتند. این یافتهها نشان میدهند که چندشکلیهای ژن IGF-I ممکن است به عنوان نشانگرهای DNA برای انتخاب در فرآیندهای اصلاح نژادی در گوسفندان نژاد مهربان مورد استفاده قرار گیرند.
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