ارتباط چندشکلی‌های ژن IGF-I با صفات لاشه در گوسفندان نژاد مهربان با استفاده از روش SSCP

Subject Areas : Camel

S. Behzadi ¹ , S.R. Miraei-Ashtiani ² , M. Sadeghi ³ , P. Zamani ⁴ , R. Abdoli ⁵

1 - Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran
2 - Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran
3 - Department of Animal Science, Faculty of Agricultureand Natural Resources, University of Tehran, Karaj, Iran
4 - Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran
5 - Department of Animal Science, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Iran

Received: 2014-01-08 Accepted : 2014-04-30 Published : 2015-03-01

Keywords: صفات لاشه, فاکتور رشد شبه انسولین نوع 1, گوسفندان مهربان, چندشکلی‌های تک نوکلئوتیدی, چندشکلی آرایش فضایی رشته‌های منفرد,

Abstract :

انتخاب به کمک ژنتیک مولکولی با استفاده از ژن­های اختصاصی یک روش پیشنهاد شده برای بهبود ژنتیکی صفات مهم اقتصادی در دام‌ها می­باشد. ژن IGF-I ممکن است نقش­های مهمی در رشد بافت­های متفاوت شامل سلول­های عضلانی، غضروف و استخوان بازی کند. اهداف مطالعه حاضر برآورد فراوانی­های هاپلوتایپی چندشکلی­های ژن IGF-I در گوسفندان نژاد مهربان و تعیین ارتباط بین این چندشکلی­ها و صفات لاشه در 439 حیوان بودند. واکنش زنجیره­ای پلیمراز با استفاده از روش­های چندشکلی­ آرایش فضایی رشته‌های منفرد (PCR-SSCP) و توالی یابی برای شناسایی تفاوت­های ژنتیکی بکار گرفته شدند. دو چندشکلی تک نوکلئوتیدی (C114G و G116A) و دو تغییر آمینو اسیدی (S5T و G6S) با سه الگوی متفاوت در قطعه تکثیر شده از ژن IGF-I شناسایی شدند. ارتباط الگوهای ژن IGF-I با کلسترول و تریگلیسرید خون معنی­دار (05/0P<) و برای ضخامت چربی پشتی و وزن لاشه متمایل به معنی­دار (1/0P<) بود. دیگر صفات مطالعه شده تحت تأثیر معنی‌داری به وسیله الگوهای متفاوت ژن IGF-I قرار نگرفتند. این یافته­ها نشان می­دهند که چندشکلی­های ژن IGF-I ممکن است به عنوان نشانگرهای DNA برای انتخاب در فرآیندهای اصلاح نژادی در گوسفندان نژاد مهربان مورد استفاده قرار گیرند.

References:

Adam C.L., Gadd T.S., Findlay P.A. and Wathes D.C. (2000). IGF-I stimulation of luteinizing hormone secretion, IGF binding proteins (IGFBPs) and expression of mRNAs for IGFs, IGF receptors and IGFBPs in the bovine pituitary gland. J. Endocrinol. 166, 247-254.
Akers R.M. (2006). Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows. J. Dairy Sci. 89, 1222-1234.
Anderson P.T., Bergen W.G., Merkel R.A., Enright W.J., Zinn S.A., Refsal K.R. and Hawkins D.R. (1988). The relationship between composition of gain and circulating hormones in growing beef bulls fed three dietary crude protein levels. J. Anim. Sci. 66, 3059-3067.
Andrade P.C., Grossi D.A., Paz C.C., Alencar M.M., Regitano L.C. and Munari D.P. (2008). Association of an insulin-like growth factor 1 gene microsatellite with phenotypic variation and estimated breeding values of growth traits in Canchim cattle. Anim. Genet. 39, 480-485.
Barash H., Aharoni Y., Brosh A. and Holzer Z. (1998). Effect of low energy diets followed by a compensatory diet on bodyweight gain and plasma hormone concentrations in bull calves. J. Dairy Sci. 81, 250-254.
Bryne P.F. and McMullen M.D. (1996). Defining genes for agricultural traits: QTL analyses and the candidate gene approach. Probe. 7, 24-27.
Burgos S.A. and Cant J.P. (2010). IGF-I stimulates protein synthesis by enhanced signaling through mTORC1 in bovine mammary epithelial cells. Domest. Anim. Endocrinol. 38, 211-221.
Casas-Carrillo E., Prill-Adams A., Price S.G., Clutter A.C. and Kirkpatrick B.W. (1997). Relation of growth hormone and insulin-like growth factor-I genotypes with carcass traits in swine. Anim. Genet. 28, 88-93.
Curi R.A., Oliveira H.N., Silveira A.C. and Lopes C.R. (2004). Effects of polymorphic microsatellites in the regulatory region of IGF-I and GHR on growth and carcass traits in beef cattle.Anim. Genet. 6, 58-62.
Davis M.E. and Simmen R.C.M. (1997). Genetic parameter estimates for serum insulin-like growth factor concentration and performance traits in Angus beef cattle. J. Anim. Sci. 75, 317-324.
Eulalia S., Lech Z., Jolanta O., Nina S., Emilia B. and Krzyzewski J. (2006). Effect of polymorphism in IGF-I gene on production traits in Polish Holstein-Friesian cattle. Anim. Sci. Pap. Rep. 24, 225-237.
Ge W., Davis M.E., Hines H.C., Irvin K.M. and Simmen R.C.M. (2001). Associations of a genetic marker with blood serum insulin-like growth factor-I concentration and growth traits in Angus cattle. J. Aim. Sci. 79, 1757-1762.
Hines H.C., Ge W., Zhao Q. and Davis M.E. (1998). Association of genetic markers in growth hormone and insulin-like growth factor I loci with lactation traits in Holsteins. Anim. Genet. 29, 69-74.
Islam K.K., Vinsk M., Crews R.E., Okine E., Moore S.S., Crews Junior D.H. and Li C. (2009). Association analyses of a SNP in the promoter of IGF-I with fat deposition and carcass merit traits in hybrid, Angus and Charolais beef cattle. Anim. Genet. 40, 766-769.
Kim E.S., Shi X., Cobanoglu O., Weigel K., Berger P.J. and Kirkpatrick B.W. (2009). Refined mapping of twinning-rate quantitative trait loci on bovine chromosome 5 and analysis of insulin-like growth factor-I as a positional candidate gene. J. Anim. Sci. 87, 835-843.
Li C., Basarab J., Snelling W.M., Benkel B., Murdoch B., Hansen C. and Moore S.S. (2004). Assessment of positional candidate genes myf5 and IGF-I for growth on bovine chromosome 5 in commercial lines of Bos taurus. J. Anim. Sci. 82, 1-7.
Miller S.A., Dykes D.D. and Polesky H.F. (1988). A simple salting out procedure for extraction DNA from human nucleotide cells. Nucl. Acid. Res. 6, 3-4.
Moody D.E., Pomp D., Newman S. and Macheil M.D. (1996). Characterization of DNA polymorphisms in three populations of Hereford cattle and their associations with growth and maternal EPD in line 1 Herefords. J. Anim. Sci. 74, 1784-1793.
Moyes T.E. (2004). Variation in concentrations of insulin-like growth factor-I (IGF-I) in pasture-fed Holstein-Friesian cows. Ph D. Thesis. University of Melbourne, Melbourne, Australia.
Owens F.N., Dubeski P. and Hanson C.F. (1993). Factors that alter the growth and development of ruminants. J. Anim. Sci. 71, 3138-3150.
Rajkumar K., Modric T. and Murphy L.J. (1999). Impaired adipogenesis in insulin-like growth factor binding protein-I transgenic mice. J. Endocrinol. 162, 457-465.
SAS Institute. (2004). SAS^®/STAT Software, Release 6.1. SAS Institute, Inc., Cary, NC.
Schwerin M., Brockmann G., Vanselow J. and Seyfert H.M. (1995). Perspectives of molecular genome analysis in livestock improvement. Arch. Fur. Tierz. 38, 21-31.
Shen W., Wisniowski P., Ahmed L., Boyle D.W. Denne S.C. and Liechty E.A. (2003). Protein anabolic effects of insulin and IGF-I in the ovine fetus. Am. J. Physiol. Endocrinol. Metab. 284, 48-56.
Sirotkin A.V., Chrenek P., Makarevich A.V., Huba J. and Bulla J. (2000). Interrelationships between breed, growth hormone, plasma IGF-I level and meat performance in bulls of different ages. Arch. Fur. Tierz. 6, 591-596.
Yao J., Aggrey S.E., Zadworny D., Hayes J.F. and Kuhnlein U. (1996). Sequence variations in the bovine growth hormone gene characterized by single-strand conformation polymorphism (SSCP) analyses and their association with milk production traits in Holstein. Genetic. 144, 1809-1816.
Yilmaz A., Davis M.E., Hines C.H. and Chung H. (2005). Detection of two nucleotide substitutions and putative promoters in the 5’ flanking region of the ovine IGF-I gene. J. Appl. Genet. 46, 307-309.
Zhang Y., Du H., Chen J., Yang G. and Zhang X. (2008). Porcine growth differentiation factor 9 gene polymorphisms and their associations with litter size. J. Genet. Genomic. 35, 163-169.