Polymorphism of Β-Lactoglobulin (Β-Lg) Gene and Its Association with Milk Yield and Milk Composition on Senduro Goats
محورهای موضوعی : Camel
F.E. Wardani
1
,
J. Palayukan
2
,
A. Furqon
3
,
S. Suyadi
4
,
T.E. Susilorini
5
1 - Department of Animal Production, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, East Java, Indonesia
2 - Department of Animal Production, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, East Java, Indonesia
3 - Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, East Java, Indonesia
4 - Department of Animal Breeding and Reproduction, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, East Java, Indonesia
5 - Department of Animal Production, Faculty of Animal Science, Universitas Brawijaya, Malang 65145, East Java, Indonesia
کلید واژه: RFLP, PCR, genotype, SNP, allele,
چکیده مقاله :
The objective of this research was to examine the β-lactoglobulin gene polymorphism and its association with milk yield and composition in Senduro goats. A total of 60 lactation Senduro goats aged 2 to 4 years were used in this study. Milk yield and blood samples were collected from dairy goat farms in Burno and Kandangtepus Senduro villages. The 480 bp β-lactoglobulin gene DNA fragments were amplified using the polymerase chain reaction (PCR) method at 60 ˚C annealing for 35 cycles. The genotypes of the β-lactoglobulin gene were determined using the restriction fragment length polymorphism (RFLP) technique with the Sac II restriction enzyme. Association of β-lactoglobulin genotypes with milk yield and composition were analyzed using the ANOVA test. The result showed that the single nucleotide polymorphism (SNP) c.497G > A was detected. The β-lactoglobulin was polymorphic in Senduro goats. There were three genotypes (AA, AG, GG) and two alleles (A and G). The frequency of AA, AG, GG genotypes were 0.4, 0.52, 0.08, respectively, while the frequencies of A and G alleles were 0.66 and 0.34, respectively. There was no association between β-lactoglobulin gene polymorphism and milk yield and composition on Senduro goats. In conclusion, the genotype of the β-lactoglobulin gene did not effect on milk yield, protein, fat, density, lactose, salt, SnF, and total solid.
Allendorf F.W., Hohenlohe P.A. and Luikart G. (2010). Genomics and the future of conservation genetics. Genetics. 11, 697-710.
Ambarwati R., Sutopo S. and Kurnianto E. (2019). Polymorphism of β-lactoglobulin (β-LG)|SacII gene in its association to milk protein and milk production in Saanen goats. J. Indonesian Trop. Anim. Agric. 44, 346-355.
Barbosa S.B.P., Araújo I.L.M.M., Martins M.F., Silvia E.C., Jacopini L.A., Batista A.M.V. and Silvia M.V.B. (2019). Genetic association of variation in the kappa-casein and β-lactoglobulin genes with milk traits in girolando cattle. Rev. Bras. Saúde Prod. Anim. 20, 1-12.
Dincel D.H., Ardicli S., Samli H. and Balci F. (2016). Determining the frequencies of B1, B2, B3 and E alleles of the CSN1S1 gene and their effects on milk yield and composition in Saanen goats. South African J. Anim. Sci. 46, 180-190.
El-Hanafy A.A., El-Saadani M.A., Eissa M., Maharem G.M. and Khalifa Z.A. (2010). Polymorphism of β-lactoglobulin gene in Barki and Damascus and their cross bred goats in relation to milk yield. Biotechnol. Anim. Husb. 26, 1-12.
El-Hanafy A.A., Qureshi M., Sabir J., Mutawakil M., Ahmed M.M., Ashmaoui H.E., Ramadan H.A.M.I., Alsoud M.A. and Sadek M.A. (2014). DNA polymorphism studies of β-lactoglobulin gene in Saudi goats. Int. J. Biol. Biomol. Agric. Food Biotechnol. Eng. 8, 1390-1393.
El-Hanafy A.A., Qureshi M., Sabir J., Mutawakil M., Ahmed M.M., Ashmaoui H.E., Ramadan H.A.M.I., Abu-Alsoud M. and Sadek M.A. (2015). Nucleotide sequencing and DNA polymorphism studies of beta lactoglobulin gene in Native Saudi goat breeds in relation to milk yield. Czech J. Anim. Sci. 60, 132-138.
El-Shazly S.A., Ahmed M.M. and Amer S.A.M. (2017). Genetic polymorphism in some milk protein genes and its impact on milk composition of Saudi Arabian goat breeds reared in Taif region. Rom. Biotechnol. Lett. 22, 12860-12867.
El-Shazly S.A., Mahfouz M.E., Al-Otalbi S.A. and Ahmed M.M. (2012). Genetic polymorphism in β-lactoglobulin gene of some sheep breeds in the Kingdom of Saudi Arabia (KSA) and its influence on milk composition. African J. Biotecnol. 11, 4330-4337.
El-Tarabany M.S., El-Tarabany A.A. and Roushdy E.M. (2018). Impact of lactation stage on milk composition and blood biochemical and hematological parameters of dairy Baladi goats. Saudi J. Biol. Sci. 25, 1632-1638.
Escareño L., Gonzales H.S., Wurzinger M., Iñiguez L., Sölkner J. and Herrera C.M. (2012). Dairy goat production systems. Trop. Anim. Health Prod. 45, 17-34.
Feitosa T.F., Vilela V.L.R., de Almeida-Neto J.L., dos Santos A., de Morais D.F., Alves B.F., Nakashima F., Gennari S.M., Athayde S.C.R. and Pena H.F.J. (2017). High genetic diversity in Toxoplasma gondii isolates from pigs at slaughterhouses in Paraiba state, northeastern Brazil: Circulation of new genotypes and Brazilian clonal lineages. Vet. Parasitol. 244, 76-80.
Ferro M.M., Tedeschi L.O. and Atzori A.S. (2017). The comparison of the lactation and milk yield and composition of selected breeds of and goats. Transl. Anim. Sci. 1, 498-506.
Furqon A., Gunawan A., Ulupi N., Suryati T. and Sumantri C. (2017). Association of ApoVLDII gene polymoprhism with body composition in Kampung Chicken. Int. J. Poult. Sci. 16, 462-466.
Gatot G., Ihsan M.N., Budiarto A., Mudawamah M., Putri A.I. and Naufal M.N. (2019). Reproduction characters of senduro goat at lumajang sitrict east java. J. Phys. Conf. Ser. 1146(1), 1-5.
Gelasakis A.I., Angelidis A.S., Giannakou R., Filioussis G., Kalamaki M.S. and Arsenos G. (2016). Bacterial subclinical mastitis and its effect on milk yield in low-input dairy goat herds. J. Dairy Sci. 99, 3698-3708.
Gharedaghi L., Shahrbabak H.M. and Sadeghi M. (2016). Identification of novel SNP in caprine β-lactoglobulin gene. J. Genet. 95, 485-490.
Goetsch A.L., Zeng S.S. and Gipson T.A. (2011). Factors affecting goat milk production and quality. Small Rumin. Res. 101, 55-63.
Gunawan A., Nurajizah E.S., Listyarini K., Furqon A., Bilyaro W., Sumantri C., Jakaria, Akter S.H. and Uddin M.J. (2018). Association study and expression analysis of stearoyl Co-A desaturase as a candidate gene for fatty acid composition in Indonesian crossbred chickens. Int. J. Poult. Sci. 17, 348-355.
Harlt D.L. and Clark A.G. (1997). Principles of Population Genetics. Sinauer Associates, Sutherland.
Hedayat-Evrigh N., Zalpour S., Sharifi R.S., Khalkhali-Evrigh R., Navidshad B., Pourasad K. and Shirzadi H. (2020). Identification of single nucleotide polymorphism (SNP) in β-lactoglobulin gene and its association with milk composition in Iranian indigenous Khalkhali goats. Iranian J. Appl. Anim. Sci. 10, 687-692.
Idowu S.T. and Adewumi O.O. (2017). Genetic and non-genetic factors affecting yield and milk composition in goats. J. Adv. Dairy. Res. 5, 1-4.
Işik R., Bilgen G., Kosum N., Kandemir C. and Taşkin T. (2017). Polymorphism in exon 7 of β-lactoglobulin (β-LG) gene and its association with milk yield in Saanen goats. Pp. 35-41 in Proc. 2nd Int. Balkan Agric. Congr., Tekirdağ, Turkey.
Jawasreh K., Al Amareen A. and Aad P. (2019). Effect and interaction of β-lactoglobulin, kappa casein and prolactin gene on milk production and composition of Awassi sheep. Animals. 9, 1-14.
Joshi A., Bankar P.S., Kuralkar S.V., Ali S.S., Deshmukh S.G. and Kuralkar P. (2019). Genetic polymorphism of alpha-lactalbumin and beta-lactoglobulin genes in Marathwadi buffalo. Rumin. Sci. 8, 205-208.
Kahilo K.H., Shazly S.E., Khadrawy A.E. and Fattouh I. (2014). Genetic polymorphism in β-lactoglobulin gene of some goat breeds in Egypt and its influence on milk yield. Life Sci. 11, 232-238.
Karimi K., Nassiri M.T.B., Mirzadeh K., Ashayerizadeh A., Roushanfekr H. and Fayyazi J. (2009). Polymorphism of the β-lactoglobulin gene and its association with milk production traits in Iranian Najdi cattle. Iranian J. Biotechnol. 7, 82-85.
Lekerpes S.S., Jung’a J.O., Badamana M.S. and Rubensteins D.I. (2014). Genetic polymorphism of beta-lactoglobulin in kenyan small East African goat breed using PCR-RFLP and sequencing. Sci. J. Anim. Sci. 3, 233-239.
Mahmood A., Jamy M., Saqlain M., Shaiq P.A., Naqvi S.M.S. and Raja G.K. (2016). Association of β-lactoglobulin protein isoforms with milk constituents in goat breeds. J. Anim. Plant Sci. 26, 862-867.
Ministry of Agriculture. (2014). The Decision of Goat Breed Senduro Jakarta Indonesia 1055/Ktps/SR.
Mohsin A.Z., Sukor R., Selamat J., Hussin A.S.M. and Ismail I.H. (2019). Chemical and mineral composition of raw goat milk as affected by breed varieties available in Malaysia. Int. J. Food Prop. 22, 815-824.
Morammazi S., Masoudi A.A., Torshizi R.V. and Pakdel A. (2016). Differential expression of the alpha S1 casein and beta-lactoglobulin genes in defferent physiological stages of the Adani goats mammary glands. Iranian J. Biotechnol. 14, 278-285.
Nafiu L.O., Muzuni Pagala M.A., Kurniawan W. and Rahadi S. (2020). Identification of growth genes diversity of swamp buffalo using RFLP in Kabaena Island, Bombana District, Southeast Sulawesi, Indonesia. Biodiversitas. 21, 1901-1907.
Nei M. and Kumar S. (2000). Molecular Evolution and Phylogenetics, Oxford University Press, New York.
Norris D., Ngambi J.W., Benyi K. And Mbajiorgu C.A. (2011). Milk production of three exotic dairy goat genotypes in Limpopo province, South Africa. Asian J. Anim. Vet. Adv. 6, 274-281.
Praharani L., Supriyati S. and Krisnan R. (2015). Milk quality of Anglo Nubian × Etawah Grade goats and Saanen × Etawah Grade goats at first kidding period. Pp. 401-405 in Proc. 6th Int. Trop. Anim. Prod. Yogyakarta, Indonesia.
Rahmatalla S.A., Arends D., Ahmed A.S., Reissmann M. and Brockmann G.A. (2019). Whey protein polymorphisms in Sundanese goat breeds. Trop. Anim. Health Prod. 52, 1211-1222.
Raja G.K., Mahmood A., Saqlain R.M., Zafar M.M., Raja A.M., Shaiq P.A. and Naqvi S.M.S. (2019). B allele of β-lactoglobulin as a marker of dairy traits in Pakistani goat breeds. J. Anim. Plant Sci. 29, 1-7.
Rashaydeh F.S., Sholi N. and Al-Atiyat R.M. (2020). Genetic polymorphisms of milk genes (β-lactoglobulin and ĸ-casein) in indigenous Awassi and improved Awassi sheep of Palestine. Livest. Res. Rural Dev. 32, 1-11.
Sihite D.E.W., Priyanto R. and Jakaria J. (2019). Polymorphism and association of 5’UTR CAPN1 gene with growth traits in bali cattle by PCR-RFLP. Trop. Anim. Sci. J. 42, 175-179.
Susilorini T.E., Furqon A., Ridhowi A., Murthadho A., Putra N.D. and Palayukan J. (2020). Phenotypic characteristic of Doe Senduro goat in Senduro Subdistrict, Lumajang Regency. Earth Environ. Sci. 478, 1-4.
Susilorini T.E., Maylinda S., Surjowardojo P. and Suyadi S. (2014). Importance of body condition score for milk production trait in Peranakan Etawah goats. J. Biol. Agric. Healthcare. 4, 151-157.
Tai C.S., Chen Y.Y. and Chen W.L. (2016). β-lactoglobulin influences human immunity and promotes cell proliferation. BioMed. Res. Int. 2016, 1-12.
Toni F., Vincenti L., Grigoleto L., Ricci A. and Schukken Y.H. (2011). Early lactation ration of fat and milk protein percentage in milk is associated with health, milk production and survival. J. Dairy Sci. 94, 1772-1783.
Upadhyay D., Patel H.M., Kerketta S., Sahu S., Bhushan B. and Dutt T. (2014). Study of udder morphology and its relationship with production parameters in local goats of Rohilkhand region of India. Indian J. Anim. Res. 48, 615-619.
Verma M., Dige M.S., Kaushik R., Gautam D., De S. and Rout P.K. (2019). Milk composition traits in Jamunapari goats: genetic parameters and effect of CSN1S1 gene variants. Int. J. Dairy Technol. 70, 1-10.
Verma M.K., Sachdeva G.K., Yadav A.K., Prakash C. and Verma A.K. (2017). Factor affecting average milk protein percentage in Sahiwal cattle and Murrah buffalo. Environ. Ecol. 35, 611-613.
Volkandari S.D., Indriawati I. and Margawati E.T. (2016). Genetic polymorphism of kappa-casein gene in Friesian Holstein: A basic selection of dairy cattle superiority. J. Indonesian Trop. Anim. Agric. 42, 213-219.
