Evaluation of Thyroid Hormones, Blood Gases, Body Antioxidant Status, the Activity of Blood Enzymes and Bone Characteristics in Broiler Chickens with Cold Induced Ascites
محورهای موضوعی : Camelر. عبدالکریمی 1 , م.ح. شهیر 2 , م. دانشیار 3
1 - Department of Animal Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
3 - Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran
کلید واژه: Performance, Broiler, ascites, leg problem,
چکیده مقاله :
A total of 150 day old female chickens (Ross 308) were randomly allocated to 2 groups with 5 replicate and 15 chicks in each replicate to determine the effects of cold induced ascites on performance, antioxidant status, blood enzyme activities and bone metabolism. The two experimental treatments were: 1) chicks that reared under normal temperature (NT) and 2) chicks that reared under cold temperature (CT). The experiment was terminated at 42 day of chicken age. Feed intake was reduced significantly by ascites during the starter period. Weight gain of NT birds was higher than CT birds during the starter, grower, finisher and the whole period (P<0.05). Feed conversion ratio was greater for CT birds during the grower, finisher and the whole period (P<0.05). Total mortality was greater in CT birds than NT ones during the whole period (P<0.05). Cold-induced ascites increased the right ventricular, total ventricular and ventricular septum weights and right ventricular (RV)/total ventricular (TV) ratio at day 42 (P<0.05). Blood pO2, O2 saturation, pH and T4 level were lower and blood pCO2, T3 and calcium level was higher in CT birds than NT birds (P<0.05). The birds of both treatments had the same tibia length but femur length was shorter in CT birds (P<0.05). The diameter of both tibia and femur was smaller in CT birds (P<0.05). CT birds had a higher incidence of leg problems than NT ones during the whole period of the experimental phase (P<0.05). In conclusion, cold induced ascites reduced the performance, increased mortality and caused leg problems in broiler chickens.
تأثیر آسیت آلقایی توسط سرما بر عملکرد، وضعیت آنتی اکسیدانی، فعالیت آنزیم‌های خونی و متابولیسم استخوان با استفاده از 150 قطعه جوجه گوشتی ماده یکروزه (سویه راس 308) در قالب طرح کاملاً تصادفی با 2 تیمار، 5 تکرار و 15 قطعه جوجه در هر تکرار بررسی شد. تیمارهای آزمایشی جوجه‌های پرورش یافته تحت دمای طبیعی (شاهد) و تحت سرما (برای إلقای آسیت) تا 42 سن روزگی بودند. مصرف خوراک به طور معنیداری توسط شرایط سرمایی (آسیت) در دوره آغازین کاهش یافت (05/0>P). افزایش وزن پرندگان پرورش یافته تحت شرایط دمایی طبیعی در دورههای آغازین، رشد، پایانی و کل دوره به طور معنیداری بیشتر از مقدار مربوط به پرندگان تحت سرما بود (05/0>P). ضریب تبدیل خوراک پرندگان تحت تیمار سرما در دورههای رشد، پایانی و کل دوره آزمایشی بیشتر از مقدار پرندگان تیمار شاهد بود (05/0>P). تلفات ناشی از آسیت پرندگان تحت سرما نیز در کل دوره آزمایشی بالاتر از مقدار مربوط به پرندگان تیمار شاهد بود (05/0>P). همچنین آسیت إلقایی توسط سرما باعث افزایش معنیدار وزن بطن چپ، کل بطن، دیواره بطن و نسبت بطن راست به کل بطن در 42 روزگی شد (05/0>P). فشار اکسیژن، درصد اشباع شدن اکسیژن با هموگلوبین، pH و میزان T4 خون پرندگان تحت تیمار سرما کمتر از مقادیر پرندگان شاهد بود در حالیکه فشار دی اکسید کربن، میزان T3 و کلسیم خون پرندگان تحت سرما بیشتر از مقادیر پرندگان تیمار شاهد بود (05/0>P). طول استخوان تیبیا در پرندگان هر دو تیمار مشابه بود اما طول فمور در پرندگان تحت تیمار سرما کوتاهتر بود (05/0>P). قطر استخوان‌های تیبیا و فمور در پرندگان تحت تیمار سرما کمتر از پرندگان تیمار شاهد بود (05/0>P). همچنین ناهنجاری‌های پای بیشتری در پرندگان تحت تیمار سرما در کل دوره آزمایشی مشاهده شد (05/0>P). به طور کلی، آسیت إلقایی به روش سرما باعث کاهش عملکرد، افزایش تلفات و وقوع ناهنجاری‌های پا در جوجه‌های گوشتی گردید.
Arab H.A., Jamshidi R., Rassouli A., Shams G. and Hassanzadeh M.H. (2006). Generation of hydroxyl radicals during ascites experimentally induced in broiler. Br. Poult. Sci. 47(2), 216-222.
Aviagen. (2014). Ross 308: Broiler Nutrition Specification. Aviagen Inc., Huntsville, Alabama.
Balog J.M. (2003). Ascites syndrome (pulmonary hypertension syndrome) in broiler chickens: are we seeing the light at the end of the tunnel? Avian. Poult. Biol. Rev. 14, 99-126.
Bobek S., Jastrzebski M. and Pietras M. (1977). Age related changes in oxygen consumption and plasma thyroid hormone concentration in the young chicken. Gen. Comp. Endocrinol. 31, 169-174.
Bradshaw R.H., Kirkden R.D. and Broom D.M. (2002). A review of the aetiology and pathology of leg weakness in broilers in relation to welfare. Avian. Poult. Biol. Rev. 13, 45-103.
Camacho-Escobar M.A., García-López J.C., Suárez-Oporta M.E., Pinos-Rodríguez J.M., Arroyo-Ledezma J.A. and Sánchez-Bernal E.I. (2011). Effects of feed intake restriction and micronutrients supplementation on ascites mortality and leg characteristics of broilers. J. Appl. Anim. Res. 39(2), 97-100.
Closter A.M., Van As P., Groenen M.A., Vereijken A.L., Van Arendonk J.A. and Bovenhuis H. (2009). Genetic and phenotypic relationships between blood gas parameters and ascites related traits in broilers. Poult. Sci. 88, 483-490.
Daneshyar M., Kermanshahi H. and Golian A. (2009). Changes of biochemical parameters and enzyme activities in broiler chickens with cold-induced ascites. Poult. Sci. 88, 106-110.
Daneshyar M., Kermanshahi H. and Golian A. (2007). Changes of blood gases, internal organs weight and performance of broiler chickens with cold induced ascites. Res. J. Boil. Sci. 2(7), 729-735.
Daneshyar M., Kermanshahi H. and Golian A. (2012). The effects of turmeric supplementation on antioxidant status, blood gas indices and mortality in broiler chickens with T3-induced ascites . Br. Poult. Sci. 53(3), 379-385.
David B.S., Maurizio E.A. and Jeffries W. (1966). The oxyzen Bohr effects in mouse hemoglobin. Arch. Biochem. Biophys. 113, 725-729.
Dawson Y.L., Gores G.L. and Nieminen A.L. (1993). Mitochondria as a source of reactive oxygen species during reductive stress in rats hepatocytes. Am. J .Physiol. 264, 961-967.
De Smit L.,Tona K., Bruggeman V., Onagbesan O., Hassanzadeh M., Arckens L. and Decuypere E. (2005). Comparison of three lines of broilers differing in ascites susceptibility or growth rate, 2. Egg weight loss, gas pressures, embryonic heat production, and physiological hormone levels. Poult. Sci. 84, 1446-1452.
Decuypere E., Buyse J. and Buys N. (2000). Ascites in broiler chickens: exogenous and endogenous structural and functional causal factors. Worlds. Poult. Sci. J. 56, 367-376.
Duthie G.G., Wahle K.W.J. and James W.P.T. (1989). Oxidants, Antioxidant's and Cardiovascular Disease. Nutr. Res. Rev. 2, 52-62.
Fathi K.N., Ebrahim Nezhad Y., Aghdam Shahryar H., Daneshyar M. and Tanha T. (2011). Antioxidant enzyme activities characterization in pulmonary hypertension syndrome (PHS) in broilers. Res. J. Biol. Sci. 6(3), 118-123.
Gabarrou J.F., Duchump C., Williams J. and Geraert P.A. (1997). A role of thyroid hormones in the regulation of dietinduced thermogenesis in birds. Br. J. Nutr. 78, 963-973.
Guo J.L., Zheng Q.H., YIN Q.Q., Cheng W. and Jiang Y.B. (2007). Study on mechanism of ascites syndrome of broilers. Am. J .Anim. Vet .Sci. 2(3), 62-65.
Gupta A.R. (2011). Ascetic syndrome in poultry: a review. Worlds. Poult. Sci. J. 67(3), 457-468.
Hafshejani E.F., Gholami Ahangaran M. and Hosseni E. (2012). Study of blood cells, blood gases and thyroid hormones in broiler chickens suspected of ascites syndrome. Glob. Vet. 8(1), 18-21.
Han B., Yoon S.S., Su J.L, Han H.R., Qu W. and Nigussie F. (2005). Effect of low ambient temperature on the concentration of free radicals related to ascites in broiler chickens. Asian-Australas. J. Anim. Sci. 18(8), 1182-1187.
Han B., Yoon S.S., Su J.L., Han H.R., Wang M., Qu W.J. and Zhong D.B. (2004b). Effects of selenium, copper and magnesium on antioxidant enzymes and lipid peroxidation in bovine fluorosis. Asian-Australas J. Anim. Sci. 17(12), 1695-1699.
Hassanzadeh H., Bozargmehri F., Buyse J., Bruggeman V. and Decuyper E. (2004). Effect of chronic hypoxia during embryonic development on physiological functioning and on hatching and post hatching parameters related to ascites syndrome in broiler chickens. Avian. Pathol. 33, 558-564.
Huchzermeyer F.W. (2012). Broilers ascites: a review of the ascites work done at the poultry section of the Onderstepoort Veterinary Institute 1981-1990. Worlds. Poult. Sci. J. 68, 41-50.
Ipek A. and Sahan U. (2006). Effects of cold stress on broiler performance and ascites susceptibility. Asian-Australas J. Anim. Sci. 19(5), 734-738.
Iqbal M., Cawthon D., Beers K., Wideman R.F. and Bottje W.G. (2002). Antioxidant enzyme activities and mitochondrial fatty acids in pulmonary hypertension syndrome (PHS) in broilers1. Poult. Sci. 81, 252-260.
Issacks R., Goldman P. and Kim C. (1986). Studies on avian erythrocyte metabolism XIV. Effect of CO2 and pH on P50 in the chicken. Am. J. Physiol. 250, 260-266.
Julian R.J. (1993). Ascites in poultry: a review article. Avian. Pathol. 22, 419-454.
Julian R.J. (1998). Rapid growth problems: ascites and skeletal deformities in broilers. Poult. Sci. 77, 1773-1780.
Katrina M.M., Michael G.E. and David A.W.A. (2001). Rapid, simple spectrophotometric method for simultaneous detection of nitrate and nitrite. Nitric Oxide: Biol. Chem. 5(1), 62-71.
Khajali F. and Qujeq D. (2005). Relationship between growth and serum lactate dehydrogenase activity and the development of ascites in broilers subjected to skip-a-day feed restriction. Int. J. Poult. Sci. 4, 317-319.
Lin H., Decuypere E. and Buyse J. (2008). Effect of thyroid hormones on the redox balance of broiler chickens. Asian-Australas J. Anim. Sci. 21, 794-800.
Luger D., Shinder D., Rzepakovsky V., Rusal M. and Yahav S. (2001). Association between weight gain, blood parameters, and thyroid hormones and the development of ascites syndrome in broiler chickens. Poult. Sci. 80, 965-971.
Maxwell M.H. and Robertson G.W. (1997). World broiler ascites survey 1996. Poult. Int. 36, 16-19.
Maxwell M.H., Alexander I.A., Robertson J.W., Mitchell M.A. and McCorquodale C.C. (1995). Identification of tissue hypoxia in the livers of ascitic and hypoxia induced broilers using trypan blue. Br. Poult. Sci. 36, 791-798.
Moayyedian H., Asasi K., Nazifi S., Hassanzadeh M. and Ansari-Lari M. (2011). Relationship between venous blood gas parameters, thyroid hormone levels and ascites syndrome in broiler chickens exposed to cold temperature . Iran J. Vet. Res. 12(1), 31-38.
Ocak F. (2006). Ascites in broilers. J. Health. Sci. 15(1), 46-50.
Olkowski A.A., Korver D., Rathgeber B. and Classen H.L. (1999). Cardiac in index, oxyzen delivery and tissue oxyzen extraction in slow and fast growing chickens and in chickens with heart failure and ascites: a comparative study. Avian. Pathol. 28, 137-146.
Oviedo-Rondón E.O., Ferket P.R. and Havestein G.B. (2006a). Understanding long bone development in broilers and turkeys. Avian. Poult .Biol .Rev. 17(3), 77-88.
Oviedo-Rondón E.O., Ferket P.R. and Havestein G.B. (2006b). Nutritional factors that affect leg problems in broilers and turkeys. Avian. Poult. Biol. Rev. 17(3), 89-103.
Park Y. and Kehre J.P. (1991). Oxidative changes in hypoxicre oxygenated rabbit heart: a consequence of hypoxia rather than reoxygenation. Free Radic. Res. Commun. 14, 179-185.
SAS Institute. (2002). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Scheele C.W., Decuypere E., Vereijken P.F.G. and Schreurs F.J. (1992). Ascites in broilers. 2. Disturbances in the hormonal regulation of metabolic rate and fat metabolism. Poult. Sci. 71, 1971-1984.
Stojevi Z., Milinkovic-Tur S. and C. Katarina. (2000). Changes in thyroid hormones concentrations in chicken blood plasma during fattening. Veterinarski Arh. 70(1), 31-37.
Tankson J.D., Thaxton J.P. and Vizzier-Thaxton Y. (2002). Biochemical and immunological changes in chickens experiencing pulmonary hypertension syndrome caused by Enterococcus faecalis. Poult. Sci. 81, 1826-1831.
Tekeli A. (2014). Effects of ascites (pulmonary hypertension stndrome ) on blood gas, blood oximetry parameters and heart section of broilers grown at high altitude. J. Anim. Plant. Sci. 24(4), 998-1002.
Van As P., Elferink M.G., Closter A.M., Vereijken A., Bovenhuis H., Crooijmans R.P.M.A., Decuypere E. and Groenen M.A.M. (2010). The use of blood gas parameters to predict ascites susceptibility in juvenile broilers. Poult. Sci. 89, 1684-1691.
Wang Y., Guo Y., Ning D., Peng Y., Cai H., Tan J., Yang Y. and Liu D. (2012). Changes of hepatic biochemical parameters and proteomics in broiler with cold-induced ascites. J. Anim. Sci. Biotechnol. 3(41), 1-9.
Widema R.F. and Tackett C. (2000). Cardio-pulmonary function in preascitic (hypoxemic) or normal broilers reared at warm or cold temperatures: effect of acute inhalation of 100% oxygen. Poult. Sci. 79, 257-264.
Wideman R.F.J.R. and Bottje W.G. (1993). Nutrition and Technical Symposium Proceedings. Novous International Inc., St. Louis, Missouri.
Wideman R.F.J.R. (1988). Ascites in poultry. Monsanto Nutr. Update. 6, 1-7.
Williams B., Solomon S., Waddington D., Thorp B. and Farquharson C. (2000). Skeletal development in the meat-type chicken. Br. Poult. Sci. 41, 141-149.
Wolfenson D., Sklan D., Graber Y., Kedar O., Bengal I. and Hurwitz S. (1987). Absorption of protein, fatty acids and minerals in young turkeys under heat and cold stress. Br. Poult. Sci. 28(4), 739-742.
Yahav S. (2000). Relative humidity at moderate ambient temperatures: its effect on male broiler chickens and turkeys. Br. Poult. Sci. 41, 94-100.
