Effects of Probiotic on Immune Response and Intestine Morphology of Broiler Chicks Exposed to Stress Induced by Corticosterone
محورهای موضوعی : Camelف. عابد درگاهی 1 , ح. درمانی‌کوهی 2 , ر. حسن ساجدی 3 , ع. روستایی علی‌مهر 4 , س.ح. حسینی‌مقدم 5
1 - Department of Animal science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
2 - Department of Animal science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
3 - Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
4 - Department of Animal science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
5 - Department of Animal science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
کلید واژه: Stress, Probiotic, broiler chicks, Corticosterone, Bacillus subtilis spores,
چکیده مقاله :
This experiment investigated the effects of different doses of Bacillus subtilis spore as a probiotic on the immune response, intestinal morphology and ileal dry matter digestibility in broiler chicks exposed to stress induced by corticosterone (CORT). Two hundred and eighty-eight one-day-old Ross 308 male broiler chicks were randomly assigned to six treatments in a completely randomized factorial design with and without CORT injection and 3 levels (0, 0.8×106, and 1.6×106 cfu/g) of B. subtilis spore supplementation. At 7 to 9 days of age (for 3 days), the chicks received one of the subcutaneous injections of CORT or corn oil (as control) at 2 mg/kg BW twice a day. The same injections were repeated at 25 to 27 days of age. Corticosterone injection led to significant (p <0.05) changes in intestinal morphology including villus height, ratio of villus height to crypt depth, and dry matter digestibility (as measured by TiO2 marker). These same parameters, however, increased in the experimental chicks relative to the control as a result of probiotic supplementation. Heterophil/lymphocyte ratio increased (p <0.05) as a result of corticosterone injection but exhibited no significant effect of probiotic supplementation. While corticosterone injection decreased lymphocyte density in the medulla of the bursa of Fabricius, this adverse effect was reversed by probiotic supplementation, which was more effective at a 1.6 × 106 concentration than at 0.8 × 106. As a general conclusion, it may be claimed that administration of B. subtilis-based probiotic alleviates certain negative effects of the stress induced by the corticosterone injection.
این آزمایش با هدف بررسی اثرات سطوح مختلف اسپور باسیلوس سوبتلیس به عنوان پروبیوتیک روی پاسخ ایمنی، مورفولوژی روده و قابلیت هضم ایلئومی ماده خشک خوراک در جوجه‌های گوشتی در معرض تنش القایی با کورتیکوستورن انجام شد. 288 جوجه خروس نژاد رأس 308 یکروزه در 6 تیمار به صورت طرح کاملا تصادفی در قالب فاکتوریل با دو سطح تزریق کورتیکوسترون و سه سطح مصرف پروبیوتیک (0، 106×0.8 و 106×1.6 در هر گرم خوراک)اختصاص داده شدند. در سنین 7 تا 9 روزگی ( سه روز) جوجه‌ها به صورت تزریق زیر پوستی کورتیکوسترون یا روغن به مقدار 2 میلی‌گرم به ازای هر کیلو وزن بدن دو بار در روز دریافت نمودند. همین مراحل تزریق در روزهای 25 تا 27 نیز تکرار شد. تزریق کورتیکوسترون موجب تغییر معنی‌دار مورفولوژی روده شامل طول پرز و نسبت طول پرز به عمق کریپت و هضم ماده خشک گردید. این پارامترها به طور نسبی با استفاده از پروبیوتیک افزایش یافتند. نسبت هتروفیل به لمفوسیت با تزریق کورتیکوسترون افزایش یافت اما تحت تأثیر مصرف پروبیوتیک قرار نگرفت. در حالیکه با تزریق کورتیکوسترون غلظت لمفوسیت‌ها در بخش مدولای بورس کاهش یافت، این اثر منفی توسط پروبیوتیک بهبود یافت. گروه مصرف پروبیوتیک 106 × 1.6 در هر گرم خوراک تأثیر بیشتری نسبت به گروه 106 × 0.8 داشت. به عنوان نتیجه کلی، مصرف پروبیوتیک بر پایه اسپور باسیلوس سوبتلیس میتواند اثرات استرس القاء شده با کورتیکوسترون را تخفیف دهد.
Alkhalf A., Alhaj M. and Al-Homidan I. (2010). Influence of probiotic supplementation on immune response of broiler chicks. Egyptian Poult. Sci. 30(1), 271-280.
Awad W., Ghareeb K., Abdel-Raheem S. and Böhm J. (2009). Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poult. Sci. 88(1), 49-56.
Compton M.M., Gibbs P.S. and Swicegood L.R. (1990). Glucocorticoid-mediated activation of DNA degradation in avian lymphocytes. Gen. Comp. Endocrinol. 80(1), 68-79.
Cooper E.L. (1984). Stress, Immunity, and Aging. Marcel Dekker, United Kingdom.
Deng W., Dong X., Tong J. and Zhang Q. (2012). The probiotic Bacillus licheniformis ameliorates heat stress-induced impairment of egg production, gut morphology, and intestinal mucosal immunity in laying hens. Poult. Sci. 91(3), 575-582.
Deniz G., Orman A., Cetinkaya F., Gencoglu H., Meral Y. and Turkmen I. (2011). Effects of probiotic (Bacillus subtilis DSM 17299) supplementation on the caecal microflora and performance in broiler chickens. Revue Méd. Vét. 162(11), 538-545.
Fellah J.S., Jaffredo T., Nagy N. and Dunon D. (2014). Development of the avian immune system. Pp. 45-63 in Avian immunology. K.A. Schat, B. Kasper and P. Kaiser, Eds. Elsevier, London, United Kingdom.
Feng Y., Yang X., Wang Y., Li W., Liu Y., Yin R. and Yao J. (2012). Effects of immune stress on performance parameters, intestinal enzyme activity and mRNA expression of intestinal transporters in broiler chickens. Asian-Australasian J. Anim. Sci. 25(5), 701-711.
Gao Z., Wu H., Shi L., Zhang X., Sheng R., Yin F. and Gooneratne R. (2017). Study of Bacillus subtilis on growth performance, nutrition metabolism and intestinal microflora of 1 to 42 d broiler chickens. Front. Microbiol. 3(2), 109-113.
Gomes A., Quinteiro-Filho W., Ribeiro A., Ferraz-de-Paula V., Pinheiro M., Baskeville E. and Palermo-Neto J. (2014). Overcrowding stress decreases macrophage activity and increases Salmonella Enteritidis invasion in broiler chickens. Avian Pathol. 43(1), 82-90.
Guo M., Hao G., Wang B., Li N., Li R., Wei L. and Chai T. (2016). Dietary administration of Bacillus subtilis enhances growth performance, immune response and disease resistance in Cherry Valley ducks. Front. Microbiol. 7, 1-7.
Gross W.B. and Siegel H.S. (1983). Evaluation of the heterophil/lymphocyte ratio as a measure of stress in chickens. Avian Dis. 27, 972-979.
Guzman-Cedillo A.E., Corona L., Castrejon-Pineda F., Rosiles-Martínez R. and Gonzalez-Ronquillo M. (2017). Evaluation of chromium oxide and titanium dioxide as inert markers for calculating apparent digestibility in sheep. J. Appl. Anim. Res. 45(1), 275-279.
Habashy W., Milfort M., Adomako K., Attia Y., Rekaya R. and Aggrey S. (2017). Effect of heat stress on amino acid digestibility and transporters in meat-type chickens. Poult. Sci. 96(7), 2312-2319.
Hu X. and Guo Y. (2008). Corticosterone administration alters small intestinal morphology and function of broiler chickens. Asian-Australasian J. Anim. Sci. 21(12), 1773-1778.
Hu X., Guo Y., Huang B., Zhang L., Bun S., Liu D. and Jiao P. (2010). Effect of corticosterone administration on small intestinal weight and expression of small intestinal nutrient transporter mRNA of broiler chickens. Asian-Australasian J. Anim. Sci. 23, 175-181.
Lin H., Decuypere E. and Buyse J. (2004). Oxidative stress induced by corticosterone administration in broiler chickens (Gallus gallus domesticus): 1. Chronic exposure. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 139(4), 737-744.
Lin H., Sui S., Jiao H., Jiang K., Zhao J. and Dong H. (2007). Effects of diet and stress mimicked by corticosterone administration on early postmortem muscle metabolism of broiler chickens. Poult. Sci. 86(3), 545-554.
Mehaisen G.M., Eshak M.G., Elkaiaty A.M., Atta A.R.M., Mashaly M.M. and Abass A.O. (2017). Comprehensive growth performance, immune function, plasma biochemistry, gene expressions and cell death morphology responses to a daily corticosterone injection course in broiler chickens. PLoS One. 12(2), e0172684.
Muniz E., Fascina V., Pires P., Carrijo A. and Guimarães E. (2006). Histomorphology of bursa of Fabricius: Effects of stock densities on commercial broilers. Brazilian J. Poult. Sci. 8(4), 217-220.
Opalinski M., Maiorka A., Dahlke F., Cunha F., Vargas F. and Cardozo E. (2007). On the use of a probiotic (Bacillus subtilis-strain DSM 17299) as growth promoter in broiler diets. Rev. Bras. Cienc. Avic. 9(2), 99-103.
Otutumi L.K., Góis M.B., de Moraes Garcia E.R. and Loddi M.M. (2012). Variations on the efficacy of probiotics in poultry. Pp. 203-220 in Probiotic in Animals. E.C. Rigobelo, Ed. InTech, Rijeka, Croatia.
Puvadolpirod S. and Thaxton J. (2000). Model of physiological stress in chickens 4. Digestion and metabolism 1 2. Poult. Sci. 79(3), 383-390.
Quinteiro-Filho W., Ribeiro A., Ferraz-de-Paula V., Pinheiro M., Sakai M., Sá L. and Palermo-Neto J. (2010). Heat stress impairs performance parameters, induces intestinal injury, and decreases macrophage activity in broiler chickens. Poult. Sci. 89(9), 1905-1914.
Rajput I.R., Li Y.L., Xu X., Huang Y., Zhi W.C., Yu D.Y. and Li W. (2013). Supplementary effects of Saccharomyces boulardii and Bacillus subtilis B10 on digestive enzyme activities, antioxidation capacity and blood homeostasis in broiler. Int. J. Agric. Biol. 15(2), 231-237.
SAS Institute. (2008). SAS®/STAT Software, Release 9.2. SAS Institute, Inc., Cary, NC. USA.
Scanes C.G. (2016). Biology of stress in poultry with emphasis on glucocorticoids and the heterophil to lymphocyte ratio. Poult. Sci. 95(9), 2208-2215.
Sen S., Ingale S., Kim J., Kim K., Kim Y., Khong C. and Kwon I. (2011). Effect of supplementation of Bacillus subtilis LS 1-2 grown on citrus-juice waste and corn-soybean meal substrate on growth performance, nutrient retention, caecal microbiology and small intestinal morphology of broilers. Asian-Australasian J. Anim. Sci. 24(8), 1120-1127.
Sikandar A., Zaneb H., Younus M., Masood S., Aslam A., Shah M. and Rehman H. (2017). Growth performance, immune status and organ morphometry in broilers fed Bacillus subtilis supplemented diet. South African J. Anim. Sci. 47(3), 378-388.
Smeets N., Nuyens F., Van Campenhout L., Delezie E., Pannecoucque J. and Niewold T. (2015). Relationship between wheat characteristics and nutrient digestibility in broilers: Comparison between total collection and marker (titanium dioxide) technique. Poult. Sci. 94(7), 1584-1591.
Song J., Xiao K., Ke Y., Jiao L., Hu C., Diao Q. and Zou X. (2014). Effect of a probiotic mixture on intestinal microflora, morphology, and barrier integrity of broilers subjected to heat stress. Poult. Sci. 93(3), 581-588.
Virden W. and Kidd M. (2009). Physiological stress in broilers: Ramifications on nutrient digestibility and responses 1 2. J. Appl. Poult. Res. 18(2), 338-347.
Xu L., Fan Q., Zhuang Y., Wang Q., Gao Y. and Wang C. (2017). Bacillus coagulans enhance the immune function of the intestinal mucosa of yellow broilers. Rev. Bras. Cienc. Avic. 19(1), 115-122.
Yang J., Liu L., Sheikhahmadi A., Wang Y., Li C., Jiao H. and Song Z. (2015). Effects of corticosterone and dietary energy on immune function of broiler chickens. PLoS One. 10(3), e0119750.
Yegani M. and Korver D.R. (2008). Factors affecting intestinal health in poultry: A review. Poult. Sci. 87(10), 2052-2063.
Zaghari M., Zahroojian N., Riahi M. and Parhizkar S. (2015). Effect of Bacillus subtilis spore (GalliPro®) nutrients equivalency value on broiler chicken performance. Italian J. Anim. Sci. 14, 94-98.
Zhang L., Zhang L., Zeng X., Zhou L., Cao G. and Yang C. (2016). Effects of dietary supplementation of probiotic, Clostridium butyricum, on growth performance, immune response, intestinal barrier function, and digestive enzyme activity in broiler chickens challenged with Escherichia coli K88. J. Anim. Sci. Biotechnol. 7(3), 1-9.
