اثرات پروبیوتیک روی پاسخ ایمنی و ریخت‌شناسی روده در جوجه های در معرض استرس القاء شده با کورتیکوسترون

Subject Areas : Camel

ف. عابد درگاهی ¹ , ح. درمانی‌کوهی ² , ر. حسن ساجدی ³ , ع. روستایی علی‌مهر ⁴ , س.ح. حسینی‌مقدم ⁵

1 - Department of Animal science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
2 - Department of Animal science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
3 - Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran
4 - Department of Animal science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran
5 - Department of Animal science, Faculty of Agricultural Science, University of Guilan, Rasht, Iran

Received: 2018-12-23 Accepted : 2019-03-16 Published : 2020-03-01

Keywords: جوجه‌های گوشتی, پروبیوتیک, تنش, اسپور باسیلوس سوبتیلیس, کورتیکوسترون,

Abstract :

این آزمایش با هدف بررسی اثرات سطوح مختلف اسپور باسیلوس سوبتلیس به عنوان پروبیوتیک روی پاسخ ایمنی، مورفولوژی روده و قابلیت هضم ایلئومی ماده خشک خوراک در جوجه‌های گوشتی در معرض تنش القایی با کورتیکوستورن انجام شد. 288 جوجه خروس نژاد رأس 308 یکروزه در 6 تیمار به صورت طرح کاملا تصادفی در قالب فاکتوریل با دو سطح تزریق کورتیکوسترون و سه سطح مصرف پروبیوتیک (0، 106×0.8 و 106×1.6 در هر گرم خوراک)اختصاص داده شدند. در سنین 7 تا 9 روزگی ( سه روز) جوجه‌ها به صورت تزریق زیر پوستی کورتیکوسترون یا روغن به مقدار 2 میلی‌گرم به ازای هر کیلو وزن بدن دو بار در روز دریافت نمودند. همین مراحل تزریق در روزهای 25 تا 27 نیز تکرار شد. تزریق کورتیکوسترون موجب تغییر معنی‌دار مورفولوژی روده شامل طول پرز و نسبت طول پرز به عمق کریپت و هضم ماده خشک گردید. این پارامترها به طور نسبی با استفاده از پروبیوتیک افزایش یافتند. نسبت هتروفیل به لمفوسیت با تزریق کورتیکوسترون افزایش یافت اما تحت تأثیر مصرف پروبیوتیک قرار نگرفت. در حالیکه با تزریق کورتیکوسترون غلظت لمفوسیت‌ها در بخش مدولای بورس کاهش یافت، این اثر منفی توسط پروبیوتیک بهبود یافت. گروه مصرف پروبیوتیک 106 × 1.6 در هر گرم خوراک تأثیر بیشتری نسبت به گروه 106 × 0.8 داشت. به عنوان نتیجه کلی، مصرف پروبیوتیک بر پایه اسپور باسیلوس سوبتلیس می­تواند اثرات استرس القاء شده با کورتیکوسترون را تخفیف دهد.

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