Novel Single Nucleotide Polymorphisms (SNPs) in Two Oogenesis Specific Genes (BMP15, GDF9) and Their Association with Litter Size in Markhoz Goat (Iranian Angora)
محورهای موضوعی : Camelب. عارف‌نژاد 1 , ی. مهدی‌زاده 2 , آ. جوانمرد 3 , م.ج. ضمیری 4 , ع. نیازی 5
1 - Department of Animal Science, Faculty of Agriculture, University of Shiraz, Shiraz, Iran
2 - Department of Animal Science, Faculty of Agriculture, University of Shiraz, Shiraz, Iran
3 - Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran
4 - Department of Animal Science, Faculty of Agriculture, University of Shiraz, Shiraz, Iran
5 - Department of Biotechnology, Faculty of Agriculture, University of Shiraz, Shiraz, Iran
کلید واژه: polymorphism, fecundity, <, i>, BMP15<, /i>, , <, i>, GDF9<, /i>, ,
چکیده مقاله :
BMP15 and GDF9 are two oogenesis specific genes play a pivotal role in female fertility in mammals and potential for improvement of prolificacy in marker-assisted selection. The aim of present research was to investigate the variation and association between BMP15 and GDF9 polymorphism and litter size in Markhoz goats. The sequence variability of the different amplified fragments utilized for genotyping was further investigated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and sequencing. Sequencing technology allowed the detection of two nucleotide variations: A both non-functional mutation (G/A) transition at nt 1026 known as P3) and a synonymous mutation at 1049 position which for the first time was found at the BMP15 gene. Also, GDF9 gene exhibited a polymorphism on BcnI site (1159 bp) within exon 2 of amplified PCR products (glutamine/proline). Our analysis using general linear model (GLM) statistical methods showed litter size was significantly influenced by genotypes of GDF9 (P<0.01). Heterozygous genotype showed higher litter size than homozygous genotypes (P<0.01). The conformation of this founding would need large sample size and test to screen in other indigenous goats.
ژنهای کاندیدای BMP15، GDF9 دو ژن مسئول تخمک‌گذاری محسوب می‌شود که نقش کلیدی آن در عملکرد باروری پستانداران ماده به اثبات رسیده است. بنابراین می‌تواند سناریوی خوبی برای تحقیقات پیرامون بهبود باروری در برنامه‌های انتخاب بر اساس اطلاعات نشانگر باشد. هدف از پژوهش حاضر، شناسایی تنوع ژنتیکی و ارتباط جهش‌های شناخته شده در سطح دو ژن عمده ذکر شده با صفت چند‌قلوزایی در بزهای نژاد مرخز می‌باشد. برای این منظور، شناسایی جهش‌های محتمل موجود در این ژن‌ها با سه فناوری RFLP، SSCP و توالی‌یابی متعاقب انجام واکنش زنجیره پلی‌مراز صورت گرفت. از نتایج مشاهده شده این مطالعه شناسایی جهش جایگزینی گوانین/ آدنین در محل 1026 جفت بازی توالی و جهش از نوع کدون‌های مترادف در ناحیه 1049 جفت بازی از ژن BMP15 بود. همچنین، با استفاده از آنزیم محدودگر BcnI متعاقب تکثیر اختصاصی ناحیه از اگزون دوم ژن GDF9 مجددا حضور جهش از نوع جایگزینی اسید امینه گلوتامین به جای اسید آمینه پرولین اثبات شد. از نتایج جالب برگرفته از این مطالعه وجود ارتباط آماری معنی‌دار بین ژنوتیپ‌های مشاهده شده حاصل از هضم آنزیمی و فنوتیپ چند‌قلوزایی در بزهای مرخز (01/0>P) می‌باشد که در واقع پیام اصلی و کلیدی این پژوهش تظاهر چند‌قلوزایی بالا در افراد حامل ژنوتیپ هتروزیگوت برای ژن GDF9 می‌باشد. بهر‌حال پیشنهاد محققان تحقیق به تکرار آزمایش با اندازه نمونه بیشتر و بررسی اعتبار و تکرارپذیری نتایج در نژادهای بز دیگر می‌باشد.
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