In vitro Gas Production and Dry Matter Digestibility of Irradiated Pomegranate (Punica granatum) Seeds
محورهای موضوعی : Camelم. زارعی 1 , ف. خلیلی 2 , پ. شورنگ 3
1 - Department of Animal Science, Razi University, Kermanshah, Iran
2 - Department of Animal Science, Razi University, Kermanshah, Iran
3 - Radiation Applications Research School, Nuclear Science and Technology Research Institute, Atomic Energy Organization of Iran, Karaj, Iran
کلید واژه: digestibility, irradiation, condensed tannin, pomegranate seed,
چکیده مقاله :
The objective of this study was to determine the effect of irradiation of pomegranate seed (PS) on chemical composition, digestibility and kinetic of gas production. Pomegranate seeds were exposed to gamma ray (GR) and electron beam (EB) at doses of 5, 10, 15 and 20 kGy. Three ruminally fistulated rams were used for obtaining ruminal fluid for in vitro digestibility and gas production measurements. Irradiation had no effect on chemical composition of PS. Orthogonal contrast did not show any significant effect for EB irradiation on neutral detergent fiber % (NDF%), but GR irradiation of PS at low doses (i.e.; 5 and 15 kGy) increased NDF percentage (P<0.05). Irradiation decreased condensed tannin (CT) content of PS at all doses (P<0.01). Gas production potential (b) and gas production rate (c) of PS were decreased; but EB irradiation at doses of 5 and 20 kGy did not effect on gas production potential of PS. Irradiation treatment did not affect partitioning factor. Ionizing radiation decreased PS digestibility, but EB irradiation at a dose of 20 kGy and 5, 15 and 20 kGy did not change in vitro dry matter digestibility and organic matter digestibility. In conclusion, the result of this study suggests that ionizing radiation processing, especially EB irradiation, can be regarded as an efficient method in decreasing CT of pomegranate seeds.
هدف از این مطالعه تعیین اثر پرتوتابی تفاله هسته انار بر ترکیب شیمیایی، قابلیت هضم و کینتیک تولید گاز حاصل از آن بود. تفاله هسته انار در معرض پرتوهای گاما و الکترون در دزهای 5، 10، 15 و 20 کیلوگری قرار گرفتند. تعداد 3 رأس قوچ سنجابی برای جمعآوری مایع شکمبه در آزمایشات قابلیت هضم و تولید گاز درون آزمایشگاهی استفاده گردید. پرتوتابی اثری بر ترکیب شیمیایی تفاله هسته انار نداشت. پرتوتابی الکترون در مقایسات اورتوگونال اثر معنیداری بر فیبر نامحلول در شوینده خنثی نداشت، اما تفاله هسته انار پرتوتابی شده با اشعه گاما در دزهای پایین (یعنی در دزهای 5 و 15 کیلوگری) باعث افزایش فیبر نامحلول در شوینده خنثی گردید (05/0P<). پرتوتابی محتوای تانن متراکم تفاله انار را در تمامی دزها کاهش داد (01/0P<). پتانسیل تولید گاز (b) و نرخ تولید گاز (c) تفاله هسته انار کاهش یافت، اما پرتو الکترون در دزهای 5 و 20 کیلوگری اثری بر پتانسیل تولید گاز تفاله هسته انار نداشت. تیمارهای پرتوتابی اثری بر فاکتور تفکیک کننده نداشت. پرتوهای یونیزه کننده قابلیت هضم تفاله هسته انار را کاهش داد، اما پرتو الکترون در دز 20 کیلوگری و 5، 15 و 20 کیلوگری قابلیت هضم ماده خشک و قابلیت هضم ماده آلی را تغییر نداد. در نهایت نتایج این مطالعه نشان داد که فرآیند کردن با پرتوهای یونیزه کننده، به خصوص پرتوتابی با پرتو الکترون، میتواند به عنوان یک روش مطلوب در کاهش تانن متراکم تفاله هسته انار مورد توجه قرار گیرد.
AOAC. (1995). Official Methods of Analysis. 16th Ed. Association of Official Analytical Chemists, Arlington, VA, USA.
Behgar M., Ghasemi S., Naserian A., Borzoie A. and Fatollahi H. (2011). Gamma radiation effects on phenolics, antioxidants activity and in vitro digestion of pistachio (Pistachia vera) hull. Radiat. Phys. Chem. 80, 963-967.
Bhat R. and Sridhar K.R. (2008). Nutritional quality evaluation of electron beam- irradiated lotus (Nelumbo nucifera) seeds. Food Chem. 107, 174-184.
Blummel M., Steingass H. and Becker K. (1997). The relationship between in vitro gas production in vitro microbial biomass and N-15 incorporation and its implications for the prediction of voluntary feed intake of roughages. Br. J. Nutr. 77, 911-921.
Charlesby A. (1981). Crosslinking and degradation of polymers. Radiat. Phys. Chem. 18, 59-66.
Chen K.H., Huber J.T., Simas J., Theurer C.B., Yu P. and ChanS.C. (1995). Effect of enzyme treatment or steam flaking of sorghum grain on lactation and digestion in dairy cows. J. Dairy Sci. 78, 1721-1727.
Chesson A., Stewart C.S. and Wallace R.J. (1982). Influence of plant phenolic acids on growth and cellulolytic activity of rumen bacteria. Appl. Environ. Microbiol. 44, 597-603.
De Toledo T.C.F., Canniatti-Brazaca S.G., Arthur V. and Piedade S.M.S. (2007). Effects of gamma radiation on total phenolics, trypsin and tannin inhibitors in soybean grains. Radiat. Phys. Chem. 76, 1653-1656.
Dela Rosa A.M., Dela Mines A.S., Banzon R.B. and Simbul Nuguid Z.F. (1983). Radiation pretreatment of cellulose for energy production. Radiat. Phys. Chem. 22, 861-868.
Duodu K.G., Minnaar A. and Taylor J.R.N. (1999). Effect of cooking and irradiation on the labile vitamins and antinutrient content of a traditional African sorghum porridge and spinach relish. Food Chem. 66, 21-27.
Ebrahimi S.R., Nikkhah A., Sadeghi A.A. and Raisali G. (2009). Chemical composition secondary compounds ruminal degradation and in vitro crude protein digestibility of gamma irradiated canola seed. Anim. Feed Sci. Technol. 151, 184-193.
Ebrahimi-Mahmoudabad S.R. and Taghinejad-Roudbaneh M. (2011). Investigation of electron beam irradiation effects on anti-nutritional factors, chemical composition and digestion kinetics of whole cottonseed, soybean and canola seeds. Radi Phys. Chem. 80, 1441-1447.
El-Niely H.F.G. (2007). Effect of radiation processing on antinutrients, in vitro protein digestibility and protein efficiency ratio bioassay of legume seeds. Radiat. Phys. Chem. 76, 1050-1057.
Farag M.D.E.H. (1998). The nutritive value for chicks of full-fat soybeans irradiated at up to 60 kGy. Anim. Feed Sci. Technol. 73, 319-328.
Feizi R., Ghodratnama A., Zahedifar M., Danesh Mesgaran M. and Raisianzadeh M. (2005). Apparent digestibility of pomegranate seed fed to sheep. P. 222 in Proc. Br. Soc. Anim. Sci. Penicuik, England.
Galyean M.L. (1997). Laboratory Procedure in Animal Nutrition Research. Texas A and M Research and ExtensionCenter, Amarillo. USA.
Ghanbari F., Ghoorchi T., Shawrang P., Mansouri H. and Torbati-NejadN.M. (2012). Comparison of electron beam and gamma ray irradiations effects on ruminal crude protein and amino acid degradation kinetics and in vitro digestibility of cottonseed meal. Radi. Phys. Chem. 81, 672-678.
Guimaraes-Beelen P.M., Berchielli T.T., Beelen R., Araujo Filho J. and Oliveira S.G. (2006). Characterization of condensed tannins from native legumes of the Brazilian northeastern semi-arid. Sci. Agric. 63, 522-528.
Holm N.W. and Berry R.J. (1970). Manual on Radiation Dosimetry. Dekker, New York.
Jackson F.S., Barry T.N., Lascano C. and Palmer B. (1996). The extractable and bound condensed tannin content of leaves from tropical tree. J. Sci. Food Agric. 71, 103-110.
Kamalak A., Canbolat O., Gurbuz Y., Ozay O., Ozkan C.O. and Sakarya M. (2004). Chemical composition and in vitro gas production characteristics of several tannin containing tree leaves. Livest. Res. Rural Dev. Available at: http://www.lrrd.org.
Khan F., Ahmad S.R. and Kronfli E. (2006). c-Radiation induced changes in the physical and chemical properties of lignocellulose. Biomacromolecules. 7, 2303-2309.
Khosravi F., Fathi Nasri M.H., Modaresi S.J. and Fazaeli Rad A. (2012). Effect of electron beam irradiation on phenolic compound content and rumen degradability parameters of pomegranate seed pulp. Pp 2491-2495 in Proc. Nation. Cong. Ani. Poult. Sci. North. Country. Sari, Iran.
Larbi A., Smith J.W., Kurdi I.O., Adekunle I.O., Raji A.M. and Lapido D.O. (1998). Chemical composition, rumen degradation and gas production characteristics of some multipurpose fodder tree shrubs during wet and dry seasons in the humid tropics. Anim. Feed Sci. Technol. 72, 81-96.
Makkar H.P.S. (2004). Recent advances in the in vitro gas method for evaluation of nutritional quality of feed resources. Pp. 55-88 in Assessing Quality and Safety of Animal Feeds. FAO Animal Production and Health Series. Rome.
Makkar H.P.S., Blummel M. and Becker K. (1998). Application of an in vitro gas method to understand the effects of natural plant products on availability and partitioning of nutrients. Pp. 147-150 in In vitro Techniques for Measuring Nutrient Supply to Ruminants. E.R. Devaille, E. Owen, A.T. Adesogan, C. Rymer, J.A. Huntington and T.L.J. Lawrence, Eds. Edinburgh, Scotland.
McDougall E.I. (1948). Studies on ruminant saliva. I. The composition and output of sheep’s saliva. Biochem. J. 43, 99-109.
Mirzaei-Aghsaghali A., Maheri-Sis N., Mansouri H., Razeghi M.E., Mirza-Aghazadeh A., Cheraghi H. and Aghajanzadeh-Golshani A. (2011). Evaluating potential nutritive value of pomegranate processing by-products for ruminants using in vitro gas production technique. ARPN J. Agric. Biol. Sci. 6, 45-51.
Modarresi S.J., Fathi Nasri M.H., Dayani O. and Rashidi L. (2010). The effect of pomegranate seed pulp feeding on DMI, performance and blood metabolites of southern Khorasan crossbred goats. Anim. Sci. Res. 20, 123-132.
Mohmmadabadi T., Chaji M. and Tabatabaei S. (2010). The effect of tannic acid on in vitro gas production and rumen fermentation of sunflower meal. J. Anim. Vet. Adv. 9, 277-280.
Ndagurwa H.G.T. and Dube J.S. (2013). Nutritive value and digestibility of mistletoes and woody species browsed by goats in a semi-arid savanna, southwest Zimbabwe. Livest. Sci. 151, 163-170.
Ndlovu L.R. and Nherera F.V. (1997). Chemical composition and relationship to in vitro gas production of Zimbabwean browsable indigenous tree species. Anim. Feed Sci. Technol. 69, 121-129.
Nsahlai I.V., Siaw D.E.K.A. and Osuji P.O. (1994). The relationship between gas production and chemical composition of 23 browses of the genus Sesbania. J. Sci. Food Agric. 65, 13-20.
O’Donovan L. and Brooker J.D. (2001). Effect of hydrolysable and condensed tannins on growth morphology and metabolism of (Streptococcus gallolyticus), (Streptococcus caprinus) and (Streptococcus bovis). Microbiology. 147, 1025-1033.
Orskov E.R. and McDonaldI. (1979). The estimation of protein degradability in the rumen from incubation measurements weighted according to the rate of passage. J. Agric. Sci. 92, 499-503.
Parker M.L., Grant A., RigbyN.M., Belton P.S. and Taylor J.R.N. (1999). Effect of popping on the endosperm cell walls of sorghum and maize. J. Cereal. Sci. 30, 209-216.
Pekel N., Yoshii F., Kume T. and Guven O. (2004). Radiation crosslinking of biodegradable hydroxypropyl methylcellulose. Carbohydr. Polym. 55, 139-147.
Polvi J. and Nordlund K. (2014). Low-energy irradiation effects in cellulose. J. Appl. Physiol. 115, 1-8.
Prakash C.V.S. and PrakashI. (2011). Bioactive chemical constituents from pomegranate (Punica granatum) juice, seed and peel: a review. Int. J. Res. Chem. Environ. 1, 1-18.
Riley R.A. (1994). Free radicals in biology: oxidative stress and the effects of ionizing radiation. Int. J. Radi. Biol. 65, 27-33.
SAS Institute. (2002). SAS®/STAT Software, Release 9.1. SAS Institute, Inc., Cary, NC. USA.
Shabtay A., Eitam H., Tadmor Y., Orlov A., Meir A., Weinberg P., Weinberg Z.G., Chen Y., Brosh A., Izhaki I. and Kerem Z. (2008). Nutritive and antioxidative potential of fresh and stored pomegranate industrial byproduct as a novel beef cattle feed. J. Agric. Food Chem. 56, 10063-10070.
Shawrang P., Sadeghi A.A., Behgar M., Zareshahi H. and Shahhoseini G. (2011). Study of chemical compositions anti-nutritional contents and digestibility of electron beam irradiated sorghum grains. Food Chem. 125, 376-379.
Taghinejad M., Nikkhah A., Sadeghi A.A., Raisal G. and Chamani M. (2009). Effects of gamma irradiation on chemical composition, antinutritional factors, ruminal degradation and in vitro protein digestibility of full-fat soybean. Asian-Australas J. Anim. Sci. 22, 534-541.
Tahan G.H., Fathi Nasri M.H., Riasi A., Behgar M. and Farhang far H. (2012). Effect of electron radiation on degradation characteristics, ruminal and post ruminal DM and CP digestion of some plant protein resources. Iranian J. Anim. Sci. Res. 3, 422-434.
Taher-Maddah M., Maheri-Sis N., Salamatdoustnobar S. and Ahmadzadeh A. (2012). Estimating fermentation characteristics and nutritive value of ensiled and dried pomegranate seeds for ruminants using in vitro gas production technique. Open Vet. J. 2, 40-45.
Theodorou M.K., Williams B.A., Dhanoa M.S., McAllan A.B. and France J. (1994). A simple gas production method using a pressure transducer to determine the fermentation kinetics of ruminant feeds. Anim. Feed Sci. Technol. 48, 185-197.
Tilly J.M.A. and Terry R.A. (1963). A two stage technique for the in vitro digestion of forage crops. J. Br. Grassl. Soc. 18, 104-111.
Topuz A. and Ozdemir F. (2004). Influences of gamma irradiation and storage on the capsaicinoids of sun-dried and dehydrated paprika. Food. 86, 509-515.
Van Soest P.J., Robertson J.B. and Lewis B.A. (1991). Methods for dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, 3583-597.
Variyar P.S., Bandyopadhyay C. and Thomas P. (1998). Effect of gamma-irradiation on the phenolic acids of some Indian spices. Int. J. Food Sci. Technol. 33, 533-537.
