Cytotoxic Effect of Hydroalcoholic Extract from Satureja bachtiarica Bunge on Hela Cancer Cells Line
محورهای موضوعی : مجله گیاهان داروییآناهیتا اروند شوشتر 1 , حسین سازگار 2 , عبداله قاسمی پیربلوطی 3
1 - گروه بیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
2 - گروه بیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
3 - گروه گیاهان داروئی، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
کلید واژه: MTT test, Hela, Satureja bachtiarica Bunge,
چکیده مقاله :
Background & Aim:Cervical cancer is the most common female genital cancer and the second most common cancer in women in the world. Since the herbal ingredients have been used for centuries to treat cancer, purpose of the present study was to determine the cytotoxic effect of hydroalcoholic extract from Satureja bachtiarica Bungeon Hela cancer cells line. Experimental: Hela cells line and natural fibroblast cells line were cultured in RPMI 1640 and DMEM Containing fetal bovine serum and antibiotics, respectively. The cells were exposed to different doses of hydroalcoholic extract of Satureja bachtiarica Bunge (0.156, 0.312, 0.625, 1.25, and 2.5 mg/ml) and were incubated for 24, 48 and 72 hr, respectively. After the incubation period, the modified MTT colorimetric test was used in order to determine cell toxicity of the extract. Results: The results of MTT test Showed that hydroalcoholic extract of Satureja bachtiarica Bunge has dose-dependent anti-cancer effect on Hela cancer cells, so that by increasing the concentration of extract and incubation in 72h, the most percentage of cell death was observed (p < 0.05). The extract of plant didn’t show any significant cytotoxicity on natural fibroblast cell line. Therefore, it seems that its compounds will be applied in treating cancer through more researches. Recommended applications/industries: Considering the low side effects of herbal medicines, especially antioxidants including Satureja bachtiarica Bunge can be used to treat cancer with drug therapy.
مقدمه و هدف: سرطان دهانه رحم شایعترین سرطان دستگاه تناسلی زنان و دومین سرطان شایع زنان در دنیاست. با توجه به اینکه ترکیبات گیاهی قرنهاست که برای درمان سرطان استفاده میشود، هدف از این مطالعه بررسی اثر سمیت سلولی عصاره هیدروالکلی مرزه بختیاری بر روی رده سلولهای سرطانی Hela بود. روش تحقیق:رده سلولهای سرطانی Hela و رده سلولهای طبیعی فیبروبلاست به ترتیب در محیط کشت RPMI 1640 و DMEM حاوی سرم جنین گاو و آنتیبیوتیک کشت گردیدند. این سلولها در مجاورت دوزهای مختلف عصاره هیدروالکلی مرزه بختیاری (156/0، 312/0، 625/0، 25/1 و 5/2 میلیگرم بر میلیلیتر) قرار گرفتند و به مدت 24، 48 و 72 ساعت انکوبه شدند. پس از پایان مدت انکوباسیون، از روش تغییر یافته آزمون رنگسنجی MTT جهت تعیین سمیت سلولی عصاره استفاده شد. نتایج و بحث: نتایج حاصل از تستMTT نشان داد که عصاره هیدروالکلی مرزه بختیاری اثر ضد سرطانی وابسته به دوز و زمان بر سلولهای سرطانی Hela دارد، به طوری که با افزایش غلظت عصاره و انکوباسیون 72 ساعت بیشترین درصد مرگ سلولی مشاهده شد (05/0p<). عصاره گیاه سمیت سلولی قابل توجهی بر روی رده سلولهای طبیعی فیبروبلاست نشان نداد. لذا به نظر میرسد با تحقیقات بیشتر در آینده میتوان از ترکیبات آن در درمان سرطان بهره جست. توصیه کاربردی/صنعتی: با توجه به اثرات جانبی کم داروهای گیاهی بویژه آنتیاکسیدانها از جمله مرزه بختیاری میتواند در درمان سرطان در کنار دارو درمانی استفاده شود.
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