Effect of the extract from Gundelia tournefortii on immature mouse oocytes
محورهای موضوعی : مجله گیاهان دارویی
آزاده عابدی
1
(
گروه فیزیولوژی دام، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
)
لیلا روحی
2
(
گروه فیزیولوژی دام، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
)
عبداله قاسمی پیربلوطی
3
(
گروه گیاهان دارویی، دانشگاه آزاد اسلامی واحد شهرکرد، شهرکرد، ایران
)
کلید واژه: Antioxidant, Immature oocytes, In-vitro Maturation, Gundelia Tournefortii extract,
چکیده مقاله :
Background & Aim: Considering that antioxidants are known as effective free radicals scavenger, it may be able to improve the in vitro oocyte maturation and the fetal quality. This study was designed to determine the effect of Gundelia tournefortii leaves (GTE) hydro-alcoholic extract as a source of antioxidant on in vitro oocyte maturation. Experimental: Germinal vasicle (GV) were recovered from 6-8 weeks old NMRI ovaries. GV were cultured for 24 hours in maturation medium in MEMα supplemented with 7.5 IU/ml hCG, 100 mIU/ml rhFSH, 5% FCS (control group) and adding different doses of GTE extract (group I: 10 µg/ml, group II: 20 µg/ml, and group III: 40 µg/ml) and then in vitro maturation stages and resumption of meiotic in all groups was recorded by inverted microscope. Results: In group I, maturation rates were improved compared to the control group. But this difference was not significant. In group II, maturation rates showed a significant increase compared to the control group (p<0.05). In group III, also maturation rates showed a significant increase compared to the control group (p<0.05). Recommended applications/industries: The results of this study showed that the extract from Gundelia tournefortii leaves has a positive effect on oocyte maturation that is doses dependent. With increasing concentration of the extract, the rate of maturation immature oocytes increased.
مقدمه و هدف: با توجه به اینکه آنتی اکسیدانها به عنوان مهارکنندگان رادیکالهای آزاد شناخته شدند، این امکان وجود دارد میزان بلوغ آزمایشگاهی تخمک را نیز بهبود بخشند. این مطالعه به منظور بررسی اثر عصاره برگ کنگر ( Gundelia Tournefortii leaves (GTE)) به عنوان یک منبع آنتی اکسیدانی، بر میزان بلوغ تخمکها در شرایط آزمایشگاهی طراحی شد. روش تحقیق: تخمک های نارس هسته دار (Germinal vesicle: GV) از تخمدانهای موش نژاد NMRI 6-8 هفته ای برداشت شدند. GV به مدت 24 ساعت در محیط بلوغ MEMα کامل شده با 51/7 واحد/میلی لیتر hCG، 100 واحد/میلی لیتر rhFSH، 5 درصد FCS (گروه کنترل) کشت داده شدند و دوزهای مختلف عصاره GTE (10 میکروگرم/میلی لیتر: گروه 1، 20 میکروگرم/میلی لیتر: گروه 2 و 40 میکروگرم/میلی لیتر: گروه 3) اضافه گردید و سپس مراحل بلوغ آزمایشگاهی و از سرگیری میوز در همه گروه ها بوسیله میکروسکوپ معکوس مورد ارزیابی قرار گرفت. نتایج و بحث: در گروه آزمایشی 1 میزان بلوغ نسبت به گروه کنترل بهبود یافته بود اما این تفاوت معنی دار نبود. در گروه آزمایشی 2 ، میزان بلوغ نسبت به گروه کنترل افزایش معنی داری را نشان داد (p<0/05 ). در گروه آزمایشی 3 نیز میزان بلوغ نسبت به گروه کنترل افزایش معنی داری را نشان داد (p<0/05). توصیه کاربردی/صنعتی: نتایج این تحقیقنشان داد کهتاثیر عصاره آبی برگ کنگر، دارای یک اثر مثبت وابسته به دوز در بلوغ تخمک می باشد، بطوریکه با افزایش میزان غلظت عصاره، میزان بلوغ تخمک های نارس نیز افزایش می یابد. به طور کلی ما نتیجه می گیریم که اضافه کردن مقادیر مناسب از عصاره های گیاهی مانند برگ کنگر به محیط کشت بلوغ، بلوغ اووسیتها را می تواند بهبود بخشد.
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