Cytotoxic effect of hydroalcoholic extract from Thymus daenesis Celak on MCF-7 cancer cells line
محورهای موضوعی : مجله گیاهان دارویی
فاطمه صادقی سامانی
1
,
حسین سازگار
2
,
عبداله قاسمی پیربلوطی
3
1 - گروه زیست شناسی، دانشکده علوم پایه، شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
2 - گروه زیست شناسی، دانشکده علوم پایه، شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران
3 - گروه گیاهان داروئی، دانشگاه آزاد، واحد شهرکرد، شهرکرد، ایران
کلید واژه: Breast cancer, MTT test, Thymus daenesis Celak, MCF-7,
چکیده مقاله :
Background & Aim:Breast cancer is the second most common cancer in women after lung cancer. Given that the herbal ingredients are used for centuries to treat cancer,The aim of this study was to determine the cytotoxic effect of hydroalcholic extract from Thymus daenesisCelak on MCF-7 cancer cells line. Experimental: Breast cancer cells MCF-7 and natural fibroblast cells were cultured in DMEM medium containing fetal bovine serum and antibiotics. The cells were exposed to different doses ofhydroalcholic extract ofThymus daenesisCelak(0.156, 0.312, 0.625, 1.25, 2.5mg/ml)and incubated for 24, 48 and 72 hours, respectively. After incubation, the modified MTT colorimetric test was used to determine cytotoxicity. Results: The results of MTT test showed thathydroalcholic extractof Thymus daenesisCelak has dose- and time-dependent anti-cancer effect on MCF-7 cancer cells, so that by increasing the concentration and 72h incubation, the most cell death was observed (P<0.05). Plant extract did not show significant cytotoxicity on natural fibroblast cells. Then, it seems that its compounds can be used in treating cancer through more future research. Recommended applications/industrie: With regard to the increasing use of herbal medicines to treat many diseases, hydroalcholic extract of Thymus daenesis Celak can be used to treat cancer with drug therapy due to having antioxidant properties.
مقدمه و هدف: سرطان پستان دومین سرطان شایع در زنان بعد از سرطان ریه میباشد. با توجه به اینکه ترکیبات گیاهی قرنهاست که برای درمان سرطان استفاده میشوند. هدف از این مطالعه بررسی اثر سمیت سلولی عصاره هیدروالکلی آویشن دنایی بر روی رده سلولهای سرطانی MCF-7 بود. روش تحقیق: سلولهای سرطانی MCF-7 و سلولهای طبیعی فیبروبلاست در محیط کشت DMEM حاوی سرم جنین گاو و آنتیبیوتیک کشت گردیدند. این سلولها در مجاورت دوزهای مختلف عصاره هیدروالکلی آویشن دنایی (156/0، 312/0، 625/0، 25/1، 5/2 میلیگرم بر میلیلیتر) قرار گرفتند و به مدت 24، 48 و 72 ساعت انکوبه شدند. پس از پایان انکوباسیون، از روش تغییر یافته آزمون رنگ سنجی MTT جهت تعیین سمیت سلولی عصاره استفاده شد. نتایج و بحث: نتایج حاصل از تست MTT نشان داد که عصاره هیدروالکلی آویشن دنایی اثر ضد سرطانی وابسته به دوز و زمان بر سلولهای سرطانی MCF-7 دارد، به طوری که با افزایش غلظت عصاره و انکوباسیون 72 ساعت بیشترین درصد مرگ سلولی مشاهده شد ( 05/0 > P). عصاره گیاه سمیت سلولی قابل توجهی را بر روی سلولهای طبیعی فیبروبلاست نشان نداد.لذا به نظر میرسد با تحقیقات بیشتر در آینده، میتوان از ترکیبات آن در درمان سرطان بهره جست. توصیه ی کاربردی/ صنعتی:با توجه به اینکه مصرف داروهای گیاهی برای درمان بسیاری از بیماریها در حال افزایش است، عصاره هیدروالکلی آویشن دنایی به دلیل داشتن خاصیت آنتیاکسیدانی میتواند در درمان سرطان در کنار دارو درمانی استفاده شود.
Abdullaev, F. 2001. Plant-derived agents cancer,Journal of Pharmacology. 15(3): 345-354.
Afshari, J., Brook, A., and Moheghi, N. 2011. The cytotoxic effect of zingiberafficinale in breast cancer(MCF7) cell line.Ofogh-e-Danesh, 17(4): 28-33.
Aggarwal, B.B., Sundaram, C., Malani, N., and Ichikawa, H .2007. Curcumin: the Indian solid gold. Advances in Experimental Medicine and Biology, 595: 1-75.
Angel, S., Morana, A., Salvatore, A., Zappia, V., and Galletti, P. 2009. Protective effect of polyphenols from Glycyrrhizaglabra against oxidative stress in Caco-2 cells. Journal of Medicinal Food, 12; 1326–1333.
Barazandeh, M., andBagherzadeh, K. 2007. Investigation on the Chemical Composition of the Essential of Thymus daenensis Celak from Four Different Regions of Isfahan Province. Journal of Medicinal Plant, 3 (23): 15-19.
Diaz-Cruz, E.S., Shapiro, C.L., and Brueggemeier, R.W.2005. Cyclooxygenase inhibitors suppress aromatase expression and activity in breast cancer cells. Journal of Clincal Endocrinology & Metabolism, 90(5): 2563-2570.
Dixon, M.J. Role of ErbB2 in selection for adjuvant tamoxifen or aromatase inhibitors.2008.Womens Health, 14(3): 229-231.
Forouzandeh, F., Salimi, S., Naghsh, N., Zamani, N., and Jahani, S.2014.Evaluation of anti-cancer effect of Peganumharmala L hydroalcholic extract on human cervical carcinoma epithelial cell line. Journal of Shahrekord University of Medical Sciences, 16(4): 1-8.
Ghasemi Pirbalouti, A., Bahmani, M., and Avijgan, M.2009. Anti-candida activity of Iranian medicinal plants. Electronic Journal of Biology, 5: 85-88.
Ghasemi Pirbalouti, A., Hashemi, M., and Ghahfarokhi, F.T. 2013. Essential oil and chemical compositions of wild and cultivated Thymus daenensis Celak and Thymus vulgaris L. Industrial Crops and Products, 48: 43-48.
Ghasemi Pirbalouti, A., Malekpoor, F., Enteshari, S., Yousefi, M., Momtaz, H., and Hamedi, B. 2010. Antibacterial activity of some folklore medicinal plants used by Bakhtiari tribal in Southwest. International Journal of Biology, 2: 55-63.
Golparvar, A., Ghasemipirbalouti, A., Zinaly, H.,and Hadipanah, A. 2012. Effect OF Harvest Times on Quantity (Morphological) and Quality Characteristics of Thymus daenensis Celak. In Isfaha. Journal of Herbal Drugs, 2(4): 245-254.
Gordanian, B., Behbahani, M., Carapetian, J., and Fazilati, M. 2014. In vitro evaluation of cytotoxic activity of flower, leaf, stem and root extracts of five Artemisia species. Research in Pharmaceutical Sciences, 9:91-96.
Hamta, A., and Ghazaghi, S. 2014. The study of Thymus vulgaris Cytotoxicity effects on breast cancer cell's line. Quarterly Journal of Sabzevar University of Medical Sciences, 21(1):122-130.
Hamta, A., and Parvini, P. 2011. Study of cytotoxic effects of Taxol and Rosemary extracts on cancerous cells derived from DMB Ainduced breast cancer in SD rats. Journal of Cell & Tissue, 2: 117-126.
Karimi, A., Ghasemi pirbalouti, A., Malekpoor, F., Yousefi, M,, and Golparvar, A. 2010. Evaluation of Ecotype and Chemotype Diversity of Thymus Daenensis Celak on Isfahan and Chaharmahal va Bakhtiari Provinces. Journal of Herbal Drugs, 1(3): 1 – 10.
Keramati, k., Sanaie, k., Babakhani, A,. Rakhshan, M., Vaezi, G., and Hareri, A. Effect of Thymus Vulgaris hydro- alcoholic extraction on DMBA induced prostate cancer Wistar rat. 2011. Research in Medicine, 35(3): 135-140.
Kumaran, A., and Karunakaran, R.J. 2006. Antioxidant and free radical scavenging activity of an aqueous extract of Coleus aromaticus. Food Chemistry,97: 109-114.
Mahdian, D., Hosseini, A., Mousavi, H., Bihamta, M., and Vahedi, M. 2015. The Evalution of the Effects of Hydro-Alcolic Extract of Brassica Olerace (Red cabbge) on Growth inhibition and Apoptosis indution in Breast cancer cell line MCF-7. Iranian Journal of Obstetrics Gynecology and Infertility, 18 (151):1-11.
Mosmann, T. 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 1983; 65: 55-63.
O'Hara, M., Kiefer, D., Farrell, K., and Kemper, K. 1998. A review of 12 commonly used medicinal herbs. Archives of Family Medicine, 7(7): 523-536.
Osborne, C.K, Hobbs, K., and Trent, J.M. 1987. Biological differences among MCF-7 human breast cancer cell lines from different laboratories.Breast Cancer Research and Treatment, 9(12):111-121.
Rana, P., and Soni, G.2008.Antioxidantpotential of thyme extract: alleviation of Nnitrosodiethylamine - in duced oxidative stress.Human & Experimental Toxicology. 27(3): 215- 221.
Rezaei, F., Shokrzadeh, M., Majd, A., andNezhadsattari, T. 2014.Cytotoxic Effect of Hydroalcoholic Extract of Cornus mas Lfruit on MCF7, HepG2 and CHO cell line by MTTAssay. Journal of Mazandaran University Medical Sciences, 24(113):130-138.
Sefidkon, F., and Ahmadi, S.h. 2000. Essential oil of SaturejakhuzistanicaJamzad. Journal of Essential Oil Research, 12: 427-428.
Shun, Y.M., Wen, Y.H., Yong, C.Y., and Jian, G.S. Two Benzyl Dihydroflavones from Phellinus Igniarius. 2003. Chinese Chemical Letters, 14(8): 810-813.
Siegel, R., Ward, E., Brawley, O., and Jemal, A. 2011. Cancer statistics, 2011: the impact of eliminating socioeconomic and racial disparities on premature cancer deaths, Cancer Journal for Clinicians. 61(4): 212-236.
Singh, B., andLucci, A. 2002. Role of cycloxygenase-2 in breast cancer,Journal of Surgical Research. 108: 173-179.
