Cytotoxic effect of hydroalcoholic extract from Thymus daenesis Celak on MCF-7 cancer cells line

محورهای موضوعی : مجله گیاهان دارویی

فاطمه صادقی سامانی ¹ (گروه زیست شناسی، دانشکده علوم پایه، شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران)
حسین سازگار ² (گروه زیست شناسی، دانشکده علوم پایه، شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران)
عبداله قاسمی پیربلوطی ³ (گروه گیاهان داروئی، دانشگاه آزاد، واحد شهرکرد، شهرکرد، ایران)

تاریخ دریافت : 1394/01/31 تاریخ پذیرش : 1394/10/30 تاریخ انتشار : 1395/04/11

کلید واژه: Breast cancer, MTT test, Thymus daenesis Celak, MCF-7,

چکیده مقاله :

Background & Aim:Breast cancer is the second most common cancer in women after lung cancer. Given that the herbal ingredients are used for centuries to treat cancer,The aim of this study was to determine the cytotoxic effect of hydroalcholic extract from Thymus daenesisCelak on MCF-7 cancer cells line. Experimental: Breast cancer cells MCF-7 and natural fibroblast cells were cultured in DMEM medium containing fetal bovine serum and antibiotics. The cells were exposed to different doses ofhydroalcholic extract ofThymus daenesisCelak(0.156, 0.312, 0.625, 1.25, 2.5mg/ml)and incubated for 24, 48 and 72 hours, respectively. After incubation, the modified MTT colorimetric test was used to determine cytotoxicity. Results: The results of MTT test showed thathydroalcholic extractof Thymus daenesisCelak has dose- and time-dependent anti-cancer effect on MCF-7 cancer cells, so that by increasing the concentration and 72h incubation, the most cell death was observed (P<0.05). Plant extract did not show significant cytotoxicity on natural fibroblast cells. Then, it seems that its compounds can be used in treating cancer through more future research. Recommended applications/industrie: With regard to the increasing use of herbal medicines to treat many diseases, hydroalcholic extract of Thymus daenesis Celak can be used to treat cancer with drug therapy due to having antioxidant properties.

چکیده انگلیسی:

مقدمه و هدف: سرطان پستان دومین سرطان شایع در زنان بعد از سرطان ریه می­باشد. با توجه به اینکه ترکیبات گیاهی قرن­هاست که برای درمان سرطان استفاده می­شوند. هدف از این مطالعه بررسی اثر سمیت سلولی عصاره هیدروالکلی آویشن دنایی بر روی رده سلول­های سرطانی MCF-7 بود. روش تحقیق: سلول­های سرطانی MCF-7 و سلول­های طبیعی فیبروبلاست در محیط کشت DMEM حاوی سرم جنین گاو و آنتی­بیوتیک کشت گردیدند. این سلول­ها در مجاورت دوزهای مختلف عصاره هیدروالکلی آویشن دنایی (156/0، 312/0، 625/0، 25/1، 5/2 میلی­گرم بر میلی­لیتر) قرار گرفتند و به مدت 24، 48 و 72 ساعت انکوبه شدند. پس از پایان انکوباسیون، از روش تغییر یافته آزمون رنگ سنجی MTT جهت تعیین سمیت سلولی عصاره­ استفاده شد. نتایج و بحث: نتایج حاصل از تست MTT نشان داد که عصاره­ هیدروالکلی آویشن دنایی اثر ضد سرطانی وابسته به دوز و زمان بر سلول­های سرطانی MCF-7 دارد، به طوری که با افزایش غلظت عصاره و انکوباسیون 72 ساعت بیشترین درصد مرگ سلولی مشاهده شد ( 05/0 > P). عصاره گیاه سمیت سلولی قابل توجهی را بر روی سلول­های طبیعی فیبروبلاست نشان نداد.لذا به نظر می­رسد با تحقیقات بیشتر در آینده، می­توان از ترکیبات آن در درمان سرطان بهره جست. توصیه­ ی کاربردی/ صنعتی:با توجه به اینکه مصرف داروهای گیاهی برای درمان بسیاری از بیماری­ها در حال افزایش است، عصاره هیدروالکلی آویشن دنایی به دلیل داشتن خاصیت آنتی­اکسیدانی می­تواند در درمان سرطان در کنار دارو درمانی استفاده شود.

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