Antidiabetes, antidyslipidemia, hemoprotective, nehproprotective and hepatoprotective effect of ethanol extract of Jatropha tanjorensis leaf against alloxan induced diabetes in rats
محورهای موضوعی : مجله گیاهان دارویی
چیکا اونبو
1
(گروه شیمی/بیوشیمی، دانشکده علوم صنعتی و کاربردی، پلی تکنیک فدرال، نیجریه)
اوبینا آجا
2
(گروه بیوشیمی، کالج علوم طبیعی، دانشگاه کشاورزی مایکل اوکپارا.)
چینوندو نوروم
3
(گروه فارماکوگنوزی و طب سنتی، دانشکده علوم دارویی، دانشگاه ننامدی آزیکوه، آکوا، نیجریه)
جوزفات اونووسونیه
4
(گروه بیوشیمی/میکروبیولوژی، دانشکده علوم صنعتی و کاربردی، پلی تکنیک فدرال ، نیجریه.)
کیوما دورو
5
(گروه فناوری دارویی، دانشکده علوم صنعتی و کاربردی، پلی تکنیک فدرال، نیجریه.)
اوبینا جوئل
6
(گروه بیوشیمی/میکروبیولوژی، دانشکده علوم صنعتی و کاربردی، پلی تکنیک فدرال ، نیجریه.)
کلید واژه: Hematology, Antidiabetic, Hepatoprotective, Nephroprotective, Antidyslipidemic,
چکیده مقاله :
Background & Aim: Management/ treatment of illness and maintenance of well-being using herbal medicines is the oldest and most popular form of healthcare practice known to humankind that has been practiced by all cultures in all ages throughout the history of evolution. The ethanol extract of Jatropha tanjorensis leave was evaluated for it pharmacological potency in alloxan-induced diabetes in albino rats.Experimental: Twenty-four adult male albino rats weighing 120-180g were randomly divided into six groups of four rats per group. Group I (Normal control) were given 0.2 mL of water. Group II (Negative control): were untreated diabetic rats. Group III were Diabetic rats treated with reference drug (glibenclamide at 5 mg/kg b.wt) which served as positive control. Group IV – VI were Diabetic rats treated with Jatropha tanjorensis leaf extract at a dose of 200 mg/kg b.wt, 400 mg/kg b.wt, and 600 mg/kg b.wt, respectively. Administrations were done orally for 14 days. Blood was collected from the tail of the rats to determine the blood glucose level on the 4th, 9th and 14th day of the study.Results: The extract significantly reduced the blood glucose level. The Jatropha tanjorensis leaf extract showed dose dependent significant (P<0.05) decrease in the triacylglycerol (TAG), low density lipoprotein (LDL), Cholesterol, total white blood cell (TWBC), aspartate aminotransferase (AST), alanine aminotransferase (ALT), bilirubin, urea, creatinine level as well as significant (P<0.05) increase in hemoglobin (Hb), packed cell volume (PCV), red blood cell (RBC) and albumin level when compared with diabetic untreated group (negative control). Diabetic-related alteration in rat serum biochemical indices were significantly improved by the extract.Recommended applications/industries: The outcome of the research gave credence to the folk use Jatropha tanjorensis leaf in the treatment of diabetes and its health related dysfunctions.
Background & Aim: Management/ treatment of illness and maintenance of well-being using herbal medicines is the oldest and most popular form of healthcare practice known to humankind that has been practiced by all cultures in all ages throughout the history of evolution. The ethanol extract of Jatropha tanjorensis leave was evaluated for it pharmacological potency in alloxan-induced diabetes in albino rats.Experimental: Twenty-four adult male albino rats weighing 120-180g were randomly divided into six groups of four rats per group. Group I (Normal control) were given 0.2 mL of water. Group II (Negative control): were untreated diabetic rats. Group III were Diabetic rats treated with reference drug (glibenclamide at 5 mg/kg b.wt) which served as positive control. Group IV – VI were Diabetic rats treated with Jatropha tanjorensis leaf extract at a dose of 200 mg/kg b.wt, 400 mg/kg b.wt, and 600 mg/kg b.wt, respectively. Administrations were done orally for 14 days. Blood was collected from the tail of the rats to determine the blood glucose level on the 4th, 9th and 14th day of the study.Results: The extract significantly reduced the blood glucose level. The Jatropha tanjorensis leaf extract showed dose dependent significant (P<0.05) decrease in the triacylglycerol (TAG), low density lipoprotein (LDL), Cholesterol, total white blood cell (TWBC), aspartate aminotransferase (AST), alanine aminotransferase (ALT), bilirubin, urea, creatinine level as well as significant (P<0.05) increase in hemoglobin (Hb), packed cell volume (PCV), red blood cell (RBC) and albumin level when compared with diabetic untreated group (negative control). Diabetic-related alteration in rat serum biochemical indices were significantly improved by the extract.Recommended applications/industries: The outcome of the research gave credence to the folk use Jatropha tanjorensis leaf in the treatment of diabetes and its health related dysfunctions.
Aboyade, O.M., Yakubu, M.T., Grierson, D.S and Afolagun, A.J. 2009. Studies on the toxicological effect of the aqueous extract of the fresh and boiled barriers of solamum aculeastrum Dunal in melewistarretss. Human Experimental Toxicology, 28(12): 765-775.
Atamgbba, A.A. 2018. Total serum protein and albumin levels of wistar albino rats on administration of methanol- ethanol (1:1) leaf extracts of anacardium oxideutale and jatropha tanjorensi. International Journal of Biochemistry, Biotechnology and Allied Fields, 3(2): 20-26.
Chikezie PC, Ojiako OA, Nwufo KC. 2015. Overview of Anti-Diabetic Medicinal Plants: The Nigerian Research Experience. Journal of Diabetes and Metabolism, 6:546.
Couthand, M.G. 2015. Oedema in Kwashiorkor is caused by Hypoalbuminaemia. Paediatrics and International Child Health, 35(2): 83-89.
Dacie, J. V. and Lewis S. M. 1991. Practical Haematology 7th edition ELBS with Churchill Livingstone. Longman group U. K. pp.5-82.
Drew, B.G., Dufy, S.J. and Formosa, M.F. 2009. High density Lipoproteins modulates glucose metabolism in patients with type 2 diabetes mellitus. Circulation, 119: 2103-2111.
Eckel, R.H., S.M. Grundy and P.Z. Zimmet, 2005. The metabolic syndrome. Lancet, 365:1415-1428.
Ehimowenma, S.O. and Osagie, A.U. 2017. Phytochemical screening and anti-anaemie effects of jatrophatanjorensis leaf in protein malnourished rats. Plants Archives, 7: 509-516.
Fawcett, J.K. and Scott, J.E. 1960. A Rapid and Precise Method for the Determination of Urea. Journal of Clinical Pathology, 13: 156-159.
Gurib-Fakim, A. 2006. Medicinal plants: traditions of yesterday and drugs of tomorrow. Molecular Aspects of Medicine, 27:1-93.
Harborne, J.B. 1973. Phytochemical Methods. Chapman and Hall Limited, London. pp. 49- 88.
Iwalewa, E.O., Adewunmi, C.O., Omisore, N.O., Adebanji, O.A. and Azike, C.K. 2005. Prooxidant effects and cytoprotective potentials of nine edible vegetables in Southwest Nigeria. Journal of Medicinal Foods, 8: 539-544.
Jain, N. C. 1986. Schalm’s veterinary Haematology, 4th ed. Lea and Fabriger, Philadelphia pp. 564 – 572.
Jang, Y., Lee, S.T., Kim, T.J., Jun, J.S., Moon, J., Jung, K.H., Park, K.I., Chuk, K. and Lee S.K. 2018. High albumin level is a predictor of favourable response to immuno therapy in autoimmune encephalitis. Science Reports, 8(1):1012.
Kochmar, J.F. and Moss, D.W. 1976. Fundamentals of Clinical Chemistry, N.W. Tietz (ed). W.B. Sanders and Company, Philadelphia. pp. 604.
Kodak-Kimble, M.A., Young, L.Y., Kradjan, W.A., Guglielmo B.J. and Corelli, R.L. 2005. Applied Therapeutics: The Clinical Use of Drugs. 8th Ed.,Lippincot Williams and Wilkins, Philadelphia, pp: 487-509.
Lorke, D. 1983. A New Approach to Practical Acute Toxicity Testing. Archives of Toxicology, 55:275-287.
Malloy, H.J. and Evelyn, KA. 1937. The determination of Bilirubin with the photoelectric colorimeter. Journal of Biological Chemistry, 119:481.
Malomo, S.O. 2000. Nigeria Jatropha Biochemistry. Molecular Biology, 2: 33-35.
Mathai, K., Mahan, L.K and Escott-Stump, S. 2000. Nutrition in the adult years. In: Mahan L.K. Escott-Stump, S. (ed) Krause’s food, Nutrition and Diet therapy, 10th ed, pp. 271, 274-275.
Meyer, S.O. and Kulkarni, A.P. 2001. Hepatoxicity, In: Introduction to Biochemical Toxicology, Willey interscience, New york, pp. 20.
Mukherjee, P.K., Maiti, K., Mukherjee, K. and Houghton, P.J. 2006. Leads from Indian medicinal plants with hypoglycemic potentials. Journal of Ethnopharmacology, 106:1-28.
Oduola, T., Adeosun, G.O., Oduola, T.A., Avwioro, G.O. and Oyeniyi, M.A. 2005. Mechanism of action of Jatropha gossypifolia stems latex as a haemostatic agent. European Journal of General Medicine, 2(4):140-143.
Olayiwola, G., Iwalewa, E.O., Omobuwajo, O.R., Adebajo, A.C., Adeniyi, A.A. and Verspohl, E.J. 2004. The antidiabetic potential of Jatropha tanjorensis leaves. Nigerian Journal of Natural Products and Medicine, 8: 55-58.
Omoregie, E.S. and Osagie, A.U. 2012. Antioxidant proper of methanolic extracts of some Nigerian plants Nutritionally- Stressed Rats. Nigerian Journal of Bassie and Applied Sciences, 20(1):7.
Orhue, E.S., Idu, M., Ataman, J.E. and Ebite, L.E. 2008. Haematological and histopathological studies of Jatrophatanjorensis leaves in rabbits. Asian Journal of Biological Sciences, 1(2): 84-89.
Patrick, O.I., Cyril, O.U. and Ugwu, C.C. 2011. Phytochemcial analysis and toxicological evaluation of the methanolic extract of jatrophatanjoreness leaf. Journal of pharmacy and Bioresources, 8(2): 86-91.
Paul, S.K., Kerenaftali, K., Brian, L.T., Michael, L.W. and Kamlesh, K. 2015. Delay in treatment intensification increases the risks of cardiovascular events in patients with type 2 diabetes. Cardiovascular Diabetology, 14:100.
Reitman, S. and Frankel, S. 1957. A colorimetric method for determination of serum glutamate oxaloacetate and glutamic pyruvate transaminase. American Journal of Clinical Pathololgy. 28:56-58.
Saxena, A. and Vikram, N.K. 2004. Role of selected Indian plants in management of type 2 diabetes: A review. Journal of Alternative and Complement Medicine, 10: 369-378.
Ungaro, R., Babyetshy, M.W., Zhu, H. and Fred, J.K.S. 2012. Protein losing entherology in ulcerative colitis. Case reports in Gastroeuterology, 6(1), 177-182.
Whiting, D.R., Guariguata, L. Weil C. and Shaw, J. 2011. IDF diabetes atlas: Global estimates of the prevalence of diabetes for 2011 and 2030. Diabetes Research. Clinical Practice, 94:311-321.
Wilson, P.F., Meigs, J.B., Sulliran, L., Fox, C.S., Nathan, D.M. and D’S R.B. 2007. Prediction of inciden diabetes mellitus in middle-aged adults: The framigham offspring study. Archives of Intermediary Medicine, 167:1008-1074.
Xie, X., X. Meng, X. Zhou, X. Shu and H. Kong, 2011. Research on therapeutic effect and hemorrheology change of berberine in new diagnosed patients with type 2 diabetes combining nonalcoholic fatty liver disease. China Journal of Chinese Materia Medica, 36: 3032-3035.
