Effects of Chitosan Nanoparticles on Mice Infected with Listeria monocytogenes
محورهای موضوعی : Biotechnological Journal of Environmental Microorganismsسارا کاظمی راد 1 , میر ساسان میرپور 2 , مهدی آسمار 3 , محمدرضا رضوی 4
1 - گروه میکروبیولوژی، دانشکده علوم پایه، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران
2 - گروه میکروبیولوژی ، دانشکده علوم پایه، واحد لاهیجان ، دانشگاه آزاد اسلامی واحد لاهیجان ، لاهیجان ، ایران
3 - گروه میکروبیولوژی ، دانشکده علوم پایه، واحد لاهیجان ، دانشگاه آزاد اسلامی واحد لاهیجان ، لاهیجان ، ایران
4 - گروه انگل شناسی، انستیتو پاستور ایران، تهران، ایران
کلید واژه: Listeriosis, nanoparticles, antibacterial properties, Balb/c mice,
چکیده مقاله :
Listeria monocytogenes is the cause of listeriosis, which has many complications, especially in pregnant women.
Due to the antibiotic resistance of this bacterium, many attempts have been made to introduce different
medicinal compounds, including nanoparticles based on biological compounds. This research aimed to examine
the mechanism of the effect of chitosan nanoparticles on L. monocytogenes in vivo (in the body of a living
organism). The standard strain of L. monocytogenes (ATCC 7644) was prepared and analyzed in the Day Hospital
Laboratory (Iran). The bacteria were examined based on biochemical tests. Then, the antibacterial activity
of concentrations of 4.88 to 5000 μg/mL of chitosan nanoparticles against L. monocytogenes standard (ATCC
7644) was calculated with the investigated methods and the lowest inhibitory and bactericidal concentrations
(MIC and MBC, respectively). The effects of different nanoparticle concentrations and ampicillin in mice infected
with bacteria were also investigated. In infected mice, the therapeutic effect increased with increasing
the nanoparticle concentration, and the concentration of 156.25 μg/mL was the most effective compared to
other treatments. Also, ampicillin (10 μg) and chitosan nanoparticles with a concentration of 39.06 μg/mL had
almost the same therapeutic effect. With the timely identification of listeria contamination in pregnant women
and the proper use of chitosan nanoparticles instead of common drugs, a new solution can be found for the
treatment of listeriosis.
Listeria monocytogenes is the cause of listeriosis, which has many complications, especially in pregnant women.
Due to the antibiotic resistance of this bacterium, many attempts have been made to introduce different
medicinal compounds, including nanoparticles based on biological compounds. This research aimed to examine
the mechanism of the effect of chitosan nanoparticles on L. monocytogenes in vivo (in the body of a living
organism). The standard strain of L. monocytogenes (ATCC 7644) was prepared and analyzed in the Day Hospital
Laboratory (Iran). The bacteria were examined based on biochemical tests. Then, the antibacterial activity
of concentrations of 4.88 to 5000 μg/mL of chitosan nanoparticles against L. monocytogenes standard (ATCC
7644) was calculated with the investigated methods and the lowest inhibitory and bactericidal concentrations
(MIC and MBC, respectively). The effects of different nanoparticle concentrations and ampicillin in mice infected
with bacteria were also investigated. In infected mice, the therapeutic effect increased with increasing
the nanoparticle concentration, and the concentration of 156.25 μg/mL was the most effective compared to
other treatments. Also, ampicillin (10 μg) and chitosan nanoparticles with a concentration of 39.06 μg/mL had
almost the same therapeutic effect. With the timely identification of listeria contamination in pregnant women
and the proper use of chitosan nanoparticles instead of common drugs, a new solution can be found for the
treatment of listeriosis.
