The Impact of Rhizospheric Pseudomonas aeruginosa on the Growth of Melissa officinalis
محورهای موضوعی : Biotechnological Journal of Environmental Microorganisms
الهام کرمی
1
,
منیر دودی
2
,
زهرا رضایتمند
3
,
لادن رحیم زاده ترابی
4
1 - گروه میکروبیولوژی، واحد فلاورجان، دانشگاه آزاد اسلامی ، اصفهان ، ایران
2 - گروه میکروبیولوژی، واحد فلاورجان، دانشگاه آزاد اسلامی ، اصفهان ، ایران
3 - گروه بیولوژی ، واحد فلاورجان ، دانشگاه آزاد اسلامی ، اصفهان ، ایران
4 - گروه میکروبیولوژی ، واحد فلاورجان ، دانشگاه آزاد اسلامی ، اصفهان ، ایران
کلید واژه: Melissa officinalis, Rhizospheric, Rhizobacteria, Pseudomonas aeruginosa,
چکیده مقاله :
Microbial communities occupy a significant position in the functioning and productivity of agricultural ecosystems.
The taxonomic genus Pseudomonas encompasses a group of bacterial species that can establish mutually
beneficial partnerships with different plants. The objective of this study was to ascertain and isolate Pseudomonas
strains that were obtained from the rhizosphere soil of M. officinalis. Furthermore, an examination was conducted
on the morphological characteristics of M. officinalis that had been treated with standard and rhizospheric Pseudomonas.
The experimental treatments consisted of 3 different groups: a rhizosphere Pseudomonas inoculation
with a concentration of 106 and 109 CFU /ml, standard Pseudomonas inoculation with a concentration of 106 and
109 CFU /ml, and a control group that did not contain any bacteria. To effectively separate bacteria, specialized
culture medium, such as the King B medium, were employed. The biochemical and molecular result revealed
that the isolated strain was Pseudomonas aeruginosa. The standard Pseudomonas treatment with a concentration
of 109 CFU/ml exhibited the greatest root length of 24.1 cm, while the treatment lacking the presence of
Pseudomonas displayed the lowest root length of 12.9. Treatments with rhizospheric P. aeruginosa showed the
greatest values for stem length, as well as root and stem weight. Meanwhile, the control treatment resulted in the
lowest measurements for these mentioned characteristics. The absence of bacteria (microorganisms) is observed
in this context. The present study’s results demonstrated that the impact of rhizospheric P. aeruginosa on the
growth and quality of M. officinalis was substantial, resulting in enhanced quantitative and qualitative growth of
the plant. The highest magnitude of root length and weight was achieved through the inoculation of P. aeruginosa
Microbial communities occupy a significant position in the functioning and productivity of agricultural ecosystems.
The taxonomic genus Pseudomonas encompasses a group of bacterial species that can establish mutually
beneficial partnerships with different plants. The objective of this study was to ascertain and isolate Pseudomonas
strains that were obtained from the rhizosphere soil of M. officinalis. Furthermore, an examination was conducted
on the morphological characteristics of M. officinalis that had been treated with standard and rhizospheric Pseudomonas.
The experimental treatments consisted of 3 different groups: a rhizosphere Pseudomonas inoculation
with a concentration of 106 and 109 CFU /ml, standard Pseudomonas inoculation with a concentration of 106 and
109 CFU /ml, and a control group that did not contain any bacteria. To effectively separate bacteria, specialized
culture medium, such as the King B medium, were employed. The biochemical and molecular result revealed
that the isolated strain was Pseudomonas aeruginosa. The standard Pseudomonas treatment with a concentration
of 109 CFU/ml exhibited the greatest root length of 24.1 cm, while the treatment lacking the presence of
Pseudomonas displayed the lowest root length of 12.9. Treatments with rhizospheric P. aeruginosa showed the
greatest values for stem length, as well as root and stem weight. Meanwhile, the control treatment resulted in the
lowest measurements for these mentioned characteristics. The absence of bacteria (microorganisms) is observed
in this context. The present study’s results demonstrated that the impact of rhizospheric P. aeruginosa on the
growth and quality of M. officinalis was substantial, resulting in enhanced quantitative and qualitative growth of
the plant. The highest magnitude of root length and weight was achieved through the inoculation of P. aeruginosa
