Journal of Comparative Pathobiology: 1 (28)

Open Access Article

1 - Determination of the significance of beta-hydroxy butyrate, non esterified fatty acids and some other serum biochemical parameters as predictors of left displacement of abomasum in cattle
نوید Basiri SH Safi ایرج Norouzian عباس Rahimi Foroushani

Left displacement of abomasum (LDA) is one of the major metabolic diseases in dairy cattlewhich cause a large economic loss in dairy industry. Thus, predicting the occurrence of LDA,especially in early weeks post parturition is very important. In the present study, 14 b More

Left displacement of abomasum (LDA) is one of the major metabolic diseases in dairy cattlewhich cause a large economic loss in dairy industry. Thus, predicting the occurrence of LDA,especially in early weeks post parturition is very important. In the present study, 14 biochemicalparameters of cows with LDA before and after parturition were compared to those of healthycows (control group) using logistic regression statistical model. Changes in six parameters,including beta-hydroxy butyrate (BHB), non-esterified fatty acids (NEFA), aspartateaminotransferase (AST), calcium, sodium and potassium were significant (p<0.05) between thegroups and were included in the prediction model. Manuscript profile

Open Access Article

2 - The histomorphometric study of adult ostrich oviduct in reproductive season.
جلیل Pourhadji ایرج پوستی

The non-carinatae birds or ratites include the biggest birds known, the ostrich and the rhea. Inostrich, the reproductive age mainly begins when the female birds are 2-3 years old. The layingseason varies depending on the region and climate. During laying phase, the ost More

The non-carinatae birds or ratites include the biggest birds known, the ostrich and the rhea. Inostrich, the reproductive age mainly begins when the female birds are 2-3 years old. The layingseason varies depending on the region and climate. During laying phase, the ostrich lays 90–100eggs. The eggs have an approximally size of 155􀂰125 mm and weigh about 1500 (1200-1800)gr. The oviduct of ostrich is divided into five anatomically distinguishable regions namelyinfundibulum, magnum, isthmus, uterus, and vagina. In this evaluation histologic aspects ofoviducts of four adult ostrich were subjected to study. Results indicated that the histology of theoviduct of ostrich largely conforms to their account of avian and Rhea. In the mucous layer:primary folds are seen with secondary folds. The pseudostratified columnar or tall simplecolumnar epithelium of the mucosal fold contains ciliated and non-ciliated cells (Mucosal cells).In subepithelial, there was lamina propria with branched tubular glands. The vagina has noglands in the lamina propria. Beyond the connective tissue of the lamina propria, the oviductwall has layer of smooth musculature. The muscular layer consists of two circularly andlongitudinally directions; the inner lamina of muscular layer consists of circulary disposed fiberssome of which continue into the subepithelial connective tissue to ultimately enter the core ofthe mucosal folds; the outer lamina contains longitudinally arranged fibers and is peripherallybound by a serous covering. Manuscript profile

Open Access Article

3 - Study of the correlation between blood BHBA level and concentrate intake in calves
محسن Davoudabady آریا Badie A.R Shaghayegh فرهاد Moosakhani ابوالفضل Zarei

Carbohydrates are the main source of energy in ruminants. As a result of microbial fermentationof carbohydrates in the rumen, volatile fatty acids are produced (Acetic acid, Butyric acid,Propionic acid) which are absorbed from the ruminal wall and supply up to 75% of th More

Carbohydrates are the main source of energy in ruminants. As a result of microbial fermentationof carbohydrates in the rumen, volatile fatty acids are produced (Acetic acid, Butyric acid,Propionic acid) which are absorbed from the ruminal wall and supply up to 75% of the requiredenergy of the animal.This study was performed on 42 Holstein calves (24 males and 18 females). Blood samples weretaken weekly to evaluate blood BHBA levels. Other data about these calves such as body weight(BW) and the concentrate intake level per week was recorded at the same time. All collecteddata were analyzed by using standard software (SPSS 15.0 for Windows, SPSS, Chicago,Illinois, USA). Values of P<0.05 were considered to indicate a statistically significantdifference. Repeated measures analysis of variance showed a statistically significant differencebetween mean beta-hydroxybutyric acid levels in male and female calves in the period of thestudy. In logistic regression model, if beta-hydroxybutyric acid level is considered as adependent variable and body weight and concentrate intake level are considered as independentvariables, a statistically significant correlation is seen between these three variables as shownbelow:BHBA = 0.016 + (0.00003 × Concentrate Intake level) + (0.002 × BW)According to the results of this study, it is cleared that concentrate intake level has a significantcorrelation with BHBA levels and BHBA level has a positive direct effect on maturation ofruminal epithelium and papillae. By increasing of concentrate intake levels and blood BHBAlevels, maturation of ruminal epithelium and weaning of the calf occurs earlier. In calves thathad diarrhea or suffer from any other illness, blood BHBA levels were decreased as a result oflower level of concentrate intake. Manuscript profile

Open Access Article

4 - Effect of melatonin and vitamin C on gastric ulcer in cholestatic rats
بابک Beta - hydroxybutyric acid, concentrate, dairy calf. مرتضی Samini فریبرز Moayer پژمان Mortazavi A.A Solati

The frequency of gastrointestinal ulceration is higher in cholestatic than in healthypopulation.Melatonin is powerful antioxidant, that does not undergo redox cycling.Vitamin C isan antioxidant,that undergo redox cycling. Cysteamine is a most potent agent for inducingga More

The frequency of gastrointestinal ulceration is higher in cholestatic than in healthypopulation.Melatonin is powerful antioxidant, that does not undergo redox cycling.Vitamin C isan antioxidant,that undergo redox cycling. Cysteamine is a most potent agent for inducinggastrointestinal ulcer and its ulcerogenic effect may be due to generation of ROS, and increasingduodenal endothelin-1 concentration.The aim of this study was to evaluate the effect of pretreatment with melatonin on cysteamineinducedgastric ulcer in unoperated control (UOC), and bile duct-ligated (BDL) rats.This study have performed on 2 groups of rats: UOC and BDL. each group was divided into 4subgroups.these subgroups were treated with saline, cysteamine, vitamin C plus cysteamine andmelatonin plus cysteamine respectively.All rats were killed 24h after the last injection andstomach was prepared for calculation of j.score. In BDL group, the common bile duct wasdoubly ligated and after7 days, rats had shown overt jaundice. BDL group was treated like theUOC group .In UOC group, injection of cysteamine was associated with significant increased in j.scorecompared with saline group. injection of vitamin C and melatonin, was associated with decreasein and j.score compared with cysteamine group. In BDL group injected with cysteamine, j.scorewere significantly more sever compared with saline group. injection of vitamin C and melatoninwas associated with significant decrease in j.score compared with cysteamine group.Our results suggest that pretreatment with melatonin , protect UOC and BDL rats againstcysteamine-induced gastric ulcer possibly by ability to improve oxidative stress. Manuscript profile

Open Access Article

5 - Purification and Determination of some Characters of Dominant Isoform of GP96 Molecules from Mice Fibrosarcoma Cell Line (WEHI164)
عقیل Tabar Molla Hassan زهیر Hassan علی Mostafaie سعید Hesaraki علی Taravati

GP96 protein is a heat shock protein (HSP) with an important role in cellular immunity. Tumorderived gp96 has entered clinical trials for cancer immunotherapy. For purification of dominantisoform of gp96 molecules from mice fibrosarcoma cell line, Cultured WEHI164 cell More

GP96 protein is a heat shock protein (HSP) with an important role in cellular immunity. Tumorderived gp96 has entered clinical trials for cancer immunotherapy. For purification of dominantisoform of gp96 molecules from mice fibrosarcoma cell line, Cultured WEHI164 cell line werelysed and supernatants of cell extract were precipitated by Amoniume sulfate , In the next stage,purification was done through affinity and ione exchange chromatography. Molecular Weight ofpurified protein was estimated by SDS-PAGE and its nature was confirmed by western blot. Theresult of this study shows that, purified molecule has molecular weight between 66-68 kd whichindicates another different isoform of this molecule; a result confirmed by western blot. Manuscript profile

Open Access Article

6 - A Study of Histidine-Decarboxylase Producing Microflora in kawakawa (Euthynnus affinis)
ولی اله Koohdar ودود Razavilar عباسعلی Motalebi

Histamine poisoning is world wide food intoxication. The causative agents are biogenic amines,produced by various bacteria species. The aim of this investigation was to identify histidinedecarboxylase-producing bacteria on Kawakawa (Euthynnus affinis) hunted at the sout More

Histamine poisoning is world wide food intoxication. The causative agents are biogenic amines,produced by various bacteria species. The aim of this investigation was to identify histidinedecarboxylase-producing bacteria on Kawakawa (Euthynnus affinis) hunted at the south of Iran.Bacteriological examinations and measurement of histamine were obtained from the musclearound the gills of twenty five frozen samples. The obtained results indicated that the meanLog10 CFU/g for total microbial and psychotropic counts in samples were 4.48±0.18SE and4.44±0.17SE, respectively. Diverse bacterial isolates were identified as histidine decarboxylaseproducingbacteria. Among them, Clostridium perfringens contributed (20.9%) followed by,Klebsiella spp. (16. 8%), Proteus spp. (14.1%), Enterobacter spp. (10.5%) and other isolates(37.7%) in samples. Different levels of histamine in Kawakawa tuna were <20, 20-50, and>50ppm for 48.0, 8.0 and 44.0% of samples, respectively. Therefore, there is seafood safetyrisks in the current harvesting and post harvesting methods used in Kawakawa tuna industry andcarefully use of preventional methods to control of histamine formation is recommended. Manuscript profile

Open Access Article

7 - Study on parasitic infections of native poultry in Golestan province
میثم Mamashly SH Rqnjbar-Bahadori ابوالقاسم Safdari رضا Aghaebrahimi-Samani

Poultry breeding in free environmental situation may cause an extensive band of parasiticinfection in them. In the present study, 110 native poultry from wet regions (Gorgan,Minoodasht, Azadshahr, Aliabad katool, Kalaleh, and Galikesh) and dry regions (Gonbad-eKavoos an More

Poultry breeding in free environmental situation may cause an extensive band of parasiticinfection in them. In the present study, 110 native poultry from wet regions (Gorgan,Minoodasht, Azadshahr, Aliabad katool, Kalaleh, and Galikesh) and dry regions (Gonbad-eKavoos and agh-ghala) in 2009 were collected randomly. After necropsy of trachea anddigestive system, its includings were checked by helminth existence and mucosal intestinesamples were checked by digestion prptozoa. Moreover, external coat and surface of poultrywere studied for presence of ectoparasites. Meanwhile, blood samples of poultry were studiedfor blood protozoa.Results showed that 97.27% of native poultry in Golestan province were infected to parasitesand isolated parasites were including: Heterakis galinarum (18.18%), Subulura brumpti(7.27%), Acuaria spiralis (5.45%), Capilaria sp (1.81%), Ascaridia galli (48.18%), Syngamustrachea (15.45%), Hymenolepis sp (2.73%), Choanotaenia infundibulum (11.81%), Railietinatetragona (53.63%), Railietina cesticyllus (14.45%), Railietina echinobothrida (25.45%),Drepanidotaenia lanceolata (1.81%), Menopon gallinae (72.72%), Argas persicus (13.63%),Menocanthus stramineus (0.9%), Goniodes dissimilis (1.81%), Dermanyssus gallinae (1.81%),Haemoproteus sp (1.81%), and Eimeria spp (2.73%). Therefore, it seems that it is necessary tocontrol of parasitic infection in poultry of Golestan province is possible by applying specificmanagement method and control of their vectors. Manuscript profile

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