Oral cancer is one of the types of head and neck cancers. Five percent of all cancers occur in the head and neck, and almost half of them occur in the oral cavity. Many factors are involved in the onset and progression of oral cancer. One of the most important factors i More
Oral cancer is one of the types of head and neck cancers. Five percent of all cancers occur in the head and neck, and almost half of them occur in the oral cavity. Many factors are involved in the onset and progression of oral cancer. One of the most important factors is miRNA and also its mechanism. Mir-96 is, directly and indirectly, involved in apoptosis, proliferation, and invasion of tumors. Therefore, investigation of its role and relation to oral cancer can improve the process of diagnosis and then treatment in patients. In this study, 30 oral tumor and 30 their marginal samples were collected and histo-phatological study were done. Then, RNA extraction was performed, specific primers were designed for Mir-96 and U6 and the expression of Mir-96 was evaluated by a real-time PCR reaction. The results showed that Mir-96 expression in tumor samples was 3.44 times higher than normal ones (p < 0.001). Also, the level of Mir-96 expression was significantly higher in the tumor samples of alcoholic patients than non-alcoholic patients (p < 0.01). Also, the level of Mir-96 expression was significantly higher in tumor samples with metastases than those without metastases (p < 0.01). On the other hand, the expression of Mir-96 in the tumor sample of subjects with prior skin cancer was significantly lower than the tumor sample of subjects with no previous history of cancer (p < 0.05). Mir-96 has good potential as a diagnostic and therapeutic marker in oral cancer.
Manuscript profile
Glioblastoma multiforme (also called glioblastoma, GBM, or grade 4 astrocytoma) is uncommon cancer which accounts for more than half of the primary malignant brain tumors diagnosed. The study of the expression of genes involved in the glioblastoma can be effective in ea More
Glioblastoma multiforme (also called glioblastoma, GBM, or grade 4 astrocytoma) is uncommon cancer which accounts for more than half of the primary malignant brain tumors diagnosed. The study of the expression of genes involved in the glioblastoma can be effective in early detection, so we discussed the clinical significance and biological role of the SMC4 gene transcript in glioblastoma cancer, which is unknown. Before treatment, 50 samples of healthy tissue margin from a glioblastoma tumor were taken as a control group, and 50 samples of glioblastoma tissue were acquired as a patient group. We did RNA extraction and cDNA synthesis. qRT-PCR was used to determine SMC4 expression levels. As an internal control, ACTB was employed. Statistical analysis was performed by GraphPad Prism v.8.0.1. ROC curve was plotted.Overexpression of SMC4 (P<0.0001) in glioblastoma tissue samples was significant compared to healthy tumor margin tissue. While in age (P<0.7507) and sex (P<0.2270) no significant expression was observed. Increased expression of SMC4 gene can play an important role in the glioblastoma cancer. Our work provides a new perspective on SMC4 gene transcription with respect to ROC curve analysis as a biomarker, but needs further study.
Manuscript profile
Curcumin, a natural product of turmeric, is known for its antibacterial and antifungal properties. Candida albicans is a major fungal pathogen with high mortality rate, particularly in immunocompromised patients. This study aimed to evaluate the effect of curcumin encap More
Curcumin, a natural product of turmeric, is known for its antibacterial and antifungal properties. Candida albicans is a major fungal pathogen with high mortality rate, particularly in immunocompromised patients. This study aimed to evaluate the effect of curcumin encapsulated in nanoparticles (polymersomes) in combination with fluconazole on the expression of the MDRI gene in drug-resistant isolates of C. albicans. This descriptive cross-sectional study involved obtaining 50 clinical samples, from women with vulvovaginal infections at Al-Zahra hospital (Rasht, Iran). After identifying the strains, resistance to fluconazole was assessed using disc diffusion and broth dilution methods. Six fluconazole-resistant isolates of C. albicans were treated with ½ MIC fluconazole (control) alone and in combination with curcumin encapsulated in nanoparticles. After 24 hours, the two cell groups were cultured on Sabouraud dextrose agar (SDA) to estimate the cell death rate. The expression of the MDR1 gene was quantitatively investigated using the qRT-PCR method in treated and untreated isolates. Our finding indicated that combined therapy with ½ MIC fluconazole and curcumin encapsulated in nanoparticles (at a concentration of 400µg/ml) reduced fungal growth by up to 50% within during 24 hours. In treated cells, qRT-PCR analysis revealed a decrease in MDR1 gene expression compared to untreated cells. Curcumin appears to enhance the effectiveness of fluconazole in fluconazole-resistant isolates by reducing MDR1 gene expression.
Manuscript profile
Sanad
Sanad is a platform for managing Azad University publications
