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Open Access Article
1 - Identification and differentiation of vaccinal and virulent Newcastle disease virus in Iran by HRM analysis
Dibazar, Sh., Sheikhi, N.*, Hemmatzadeh, F., Charkhkar, S., Pourbakhsh, A. .To control of outbreaks caused Newcastle disease, it is necessary to distinguish virulent virusesfrom vaccine strains, in minimum possible time and with high accuracy. The aim of this studywas using High-Resolution Melting-Curve Analysis (HRM) for detection and differen MoreTo control of outbreaks caused Newcastle disease, it is necessary to distinguish virulent virusesfrom vaccine strains, in minimum possible time and with high accuracy. The aim of this studywas using High-Resolution Melting-Curve Analysis (HRM) for detection and differentiation ofIranian Newcastle disease virus (NDVs) isolates from vaccine strains. In this study, 5 virulentisolates along with 2 vaccine strains, including B1 and Lasota were used. Based on thenucleotide sequence of F gene, 8 primers (A - H) were designed for the analysis of HRM. At thefirst stage, one virulent and 2 vaccine virus was analyzed by 8 primer pairs. Based on thepreliminary results of both RT-PCR HRM, 3 sets of the primers have been selected for finaltesting of the samples. The patterns obtained from wild viruses were compared with vaccinegroup. The melting temperatures for vaccine strains were higher than virulent isolates by A, B,C, F and H primer pairs; despite D, E and G. In this study, B and H primer pairs could separatevaccine strains from each other and from virulent isolates, better than other primers. Based onthese results, HRM analysis and correct primer selection can determinate and differentiatevirulent NDVs from vaccine strains. This technique is able to do this in short time and with highaccuracy in comparison with previous conventional approaches such as pathogenicity indicestests, RT-PCR and nucleotide sequencing. Manuscript profile -
Open Access Article
2 - Distribution map of tomato yellow leaf curl virus in tomato field of Fars province
Ali Pakniat Sasan Ghasemi Abdollah KarborA survey was conducted to detect the distribution of tomato (yellow) leaf curl disease in tomato fileds of Fars province including Lamerd, Lar, Farashband, Kazeroon, Mamasani, Shiraz, SaadatShahr, and Abadeh region during winter, spring and summer of 2012. suspect MoreA survey was conducted to detect the distribution of tomato (yellow) leaf curl disease in tomato fileds of Fars province including Lamerd, Lar, Farashband, Kazeroon, Mamasani, Shiraz, SaadatShahr, and Abadeh region during winter, spring and summer of 2012. suspected samples that showed severe leaf curl associate with stunting and yellowing around the leaves were collected. After DNA extraction, samples were tested with specific primers of Abadeh isolate and degenerate primers of begomoviruses using polymerase chain reaction (PCR). The results showed that Lamerd, Lar, Saadatshahr, Abadeh and Mamasani regions are infected and distribution map of TYLCV was drown in Fars Province. Manuscript profile -
Open Access Article
3 - Structural and discursive analysis of primers in Najmuddin Zarkoub's Fotovvat-nāmeh based on Halliday's theory
Davud Ghanbary Narges Oskouie aziz hojjaji The initiator, as the point of departure of the message, provides the possibility of processing the text message for the speaker as well as determining the action and intellectual direction of the message for the audience. In this research, in order to analyze the More The initiator, as the point of departure of the message, provides the possibility of processing the text message for the speaker as well as determining the action and intellectual direction of the message for the audience. In this research, in order to analyze the structural and discursive text of Najmuddin Zarkoub's fotovvat-name and discover the mechanisms between the author's mind and language and the cultural-social context of the text, the analysis of primers has been used. From the analysis of the types of primers in this text, which was done using a combined method (descriptive, analytical and statistical), the following results were obtained: the significant frequency of experimental primers in this text indicates the predominance of explanatory and explanatory action in the author's discourse. The significant reduction of interpersonal initiators indicates the low level of communicative and interactive actions in the text and confirms the strengthening of monophonic and authoritative discourse meanings in the experimental structures. Among the text starters, structural elements are more prominent than the other two types (continuity and link addition) due to the role they play in the continuity and development of the text. In this text, the frequency of simple and unmarked starters is more than that of compound and marked starters, whose impact in the text can be seen in the form of ease and regularity of language and grammar, as well as textual coherence in the context of the text's ideological discourse. Manuscript profile -
Open Access Article
4 - Study on the occurrence of some important viral pathogens infecting pepper in varamin area
Azin Aghazadeh Mojdeh Maleki Ali Reza GolnaraghiSurveys were conducted to determine viral diseases of pepper plants (Capsicum annum) in different regions of Varamin county during the growing seasons of 2012-2014. A total of 110 pepper leaf samples and 11 weed leaf samples were collected from plants showing virus-like MoreSurveys were conducted to determine viral diseases of pepper plants (Capsicum annum) in different regions of Varamin county during the growing seasons of 2012-2014. A total of 110 pepper leaf samples and 11 weed leaf samples were collected from plants showing virus-like symptoms. The samples were tested by enzyme-linked immunosorbent assay (ELISA) using antibodies produced against Cucumber mosaic virus (CMV), Potato virus X (PVX), Tobacco mosaic virus (TMV), Tomato mosaic virus (ToMV), Tomato yellow leaf curl virus (TYLCV), and the broad-spectrum antibodies reacting potyviruses. According to the ELISA results, virus infections were related to TMV (26.7%), followed by potyviruses (24.3%), TYLCV (23.8%), CMV (13.3%), ToMV (13.3%) and PVX (1.9%). Nearly, 64.1% of the pepper leaf samples were infected with vector-transmitted viruses and 34.5% with viruses having no known vectors for their transmission. The results also indicated that 21.0% and 77% of the samples had single and mixed virus infections. respectively The tobamovirus and potyvirus infections of the samples were confirmed by reverse transcription-polymerase chain reaction (RT-PCR) using previously described universal primer pairs Tob-Uni1/Tob-Uni2 and NIb2F/NIb3R, which resulted in the amplification of DNA fragments with the expected sizes of 800 bp and 350 bp, respectively. Moreover, the presence of TYLCV was confirmed by the amplification of DNA fragments with the expected size of ca. 670 bp by PCR using virus-specific primers TYLCV-Sar and TYLCV-Isr. This study showed a widespread occurrence of different viruses especially tobamoviruses and potyviruses in pepper fields of Varamin area. In the present work, the potyvirus infection of morning glory plant (Ipomoea purpurea) was also confirmed. Manuscript profile -
Open Access Article
5 - Identification of microsatellite markers linked to Potato Virus Y (PVY) resistance genes in tobacco using Bulk Segregated Analysis (BSA) method
Marzieh Shazdehahmadi Mohammad Reza Salavati MeybodiOne of the most important viral diseases causing damage on tobacco is Potato Virus Y (PVY) which reduses the quality and quantity of tobacco products. The best way to control the disease is use of resistant cultivars. Molecular markers are used to label resistance genes MoreOne of the most important viral diseases causing damage on tobacco is Potato Virus Y (PVY) which reduses the quality and quantity of tobacco products. The best way to control the disease is use of resistant cultivars. Molecular markers are used to label resistance genes in breeding programs. To determine the microsatellite markers linked to PVY resistance genes in tobacco, this research was conducted in Tirtash Tobacco Research Center, Behshahr, Iran. In the first year, VAM (a resistant cultivar) was crossed with K326 (a susceptible cultivar). F1 seeds were plants and F2 seeds were obtained. F2 plants were transplanted in farm and inoculated with a pure isolate of PVY strain O. Area under disease progress curve (AUDPC) was calculated as phenotypic evaluation. To confirm resistance and susceptibility of the used cultivars’ ten plants of each cultivar were tested by ELISA and resistant and susceptible plants were selected. Using 100 SSR pair primers and BSA method, microsatellite markers linked to PVY resistance genes were determined in resistant and susceptible bulks and in F2 populations. Three primer compounds PT30002, PT20165 and PT20357 produced polymorphism on DNA of resistant and susceptible bulks. These primers were also on single plants of two bulks and F2 populations and the data were analyzed. Markers distance from resistance genes was determined by Mapmaker ver.3.0 software. Based on the results, PT3 marker was located on 17.5 CM and PT1 and PT2 on 18.1 and 37.4 CM distance from resistance gene. Manuscript profile -
Open Access Article
6 - Genetic diversity and relationships among traits in potato genotypes using agronomic traits and molecular marker (SSR).
Hossein Afshari Ehsan Barzin Ghanbar laei Morteza Noryan -
Open Access Article
7 - Prevalence and antibiotic resistance pattern of Acinetobacter spp. infections in Shahrekord medical centers
Tohid Piri Gharaghie Abbas Doosti Seyed Abbas Mirzaeithe study estimated the prevalence and pattern of antimicrobial resistance of Acinetobacter spp. isolate from different clinical specimens. Primer design for Acinetobacter spp. was performed with Bioinformatics software Primer Express and Gene Runner. Primer authenticat Morethe study estimated the prevalence and pattern of antimicrobial resistance of Acinetobacter spp. isolate from different clinical specimens. Primer design for Acinetobacter spp. was performed with Bioinformatics software Primer Express and Gene Runner. Primer authentication was performed by BLASTn online tool and Sequence Match program. Acinetobacter isolates were identified from different clinical specimens by standard biochemical methods and PCR tests. Antimicrobial susceptibility tests and biofilm detection were performed for isolates identified by standard disk diffusion method according to CLSI protocol and Microtiter plate. Acinetobacter spp. were identified by designed primer with Query Cover and Per. Ident 100%. From 60 clinical samples, 243 bacterial isolates were obtained. 131 isolates (53.9%) were related to gram-positive bacteria and 112 isolates (46.09%) were gram-negative bacteria, of which 43 isolates (17.69%) Were identified as Acinetobacter spp. According to the PCR test, 31 strains (77.5%) were identified as Acinetobacter baumannii, 7 strains (17.5%) as Acinetobacter lwoffii, 2 strains (5%) as Acinetobacter junii. Antibiotic susceptibility study showed that all isolated strains were MDR and 87.5% of isolates were XDR. However, only 12.5% of the isolates were sensitive to carbapenems, All Acinetobacter isolates were biofilm positive and were identified as strong biofilms with a total mean of 0.213. According to the study, it is clear that infection with Acinetobacter can lead to significant challenges in the country's health care system in the future. To this end, finding solutions to prevent infection of this bacterial genus, especially Acinetobacter baumannii, is very important and necessary. Manuscript profile -
Open Access Article
8 - Evaluation of NKP46 gene expression in celiac disease patients on a gluten free diet
Maryam Khodapanah Mohammad Rostami-Nejad Mehrdad Hashemi Hamid Asadzadeh-AghdaeiAccording to the previous studies, natural cytotoxicity receptors including NKP46 can play an important role in strengthening and sustaining immune responses against viral infection, which may have a role in the progression of inflammation in celiac disease. Moreover, I MoreAccording to the previous studies, natural cytotoxicity receptors including NKP46 can play an important role in strengthening and sustaining immune responses against viral infection, which may have a role in the progression of inflammation in celiac disease. Moreover, Interleukin-15, which is one of the most important inflammatory cytokines in celiac disease pathogenesis, can also change the expression of this type of receptors that leads to atrophy of intestinal villi. The aim of this study was to evaluate the intestinal expression of NKP46 gene in patients with celiac disease on a gluten-free diet in comparison with healthy individuals. In this study, 20 small intestinal biopsy samples were collected from patients with celiac disease and 20 samples from healthy subjects. After RNA extraction and cDNA synthesis, gene-specific primer pairs were designed, PCR conducted and NKP46 gene expression was evaluated by real-time PCR method. 8 females (40%) and 12 males (60%) in the patient group and 7 females (35%) and 13 males (65%) in the control group were studied. NKP46 gene expression was not significantly different between celiac patients who were on a gluten-free diet and healthy individuals (Pvalue: 0.4). The lack of significant difference in the expression of this receptor between the patient and control groups can be due to the effects of gluten-free diet adherence. Additional studies are recommended to achieve a more complete expression profile of these receptors and possible mutations affecting their expression in treated and untreated celiac disease patients. Manuscript profile -
Open Access Article
9 - Identifying the Resilience Dimensions of Principals at Primary School Based on Thematic Analysis
Mahdi Bagheri Masoud Sadeghi Mahmoud Abolghasemi Seyfollah Fazllolahi ghomshiToday, school principals face a variety of challenges that should be equipped with resilience. Therefore, the purpose of this study was to identify the resilience dimensions of primary school principals. This research was qualitative and using the thematic analysis meth MoreToday, school principals face a variety of challenges that should be equipped with resilience. Therefore, the purpose of this study was to identify the resilience dimensions of primary school principals. This research was qualitative and using the thematic analysis method. In this research, all researches associated with resilience of school principals and educational leaders were considered as the main text for extracting concepts and categories. Data were analyzed by MAXQDA2018 software and 110 themed themes were extracted. of the total basic themes, 14 dimensions were designed as predatory themes for resilient primary school principals. Predicitors included physical and psychological health, healthy personality traits, resilient measures and functional functions, relative abilities, religious and spiritual values, positive job characteristics, positive and constructive interactions with components of educational system, organizational commitment, problem and conflict management, challenge The ability to convert and convert them to the opportunity, quality of work life, perspective and organizational goals, proper environmental conditions and assistance and appropriate support for systems. The identified dimensions of primary school administrators can be used as possible components to resilience more primary school principals. Manuscript profile -
Open Access Article
10 - Full-length genome sequencing of an isolate of tomato yellow leaf curl virus to determine its taxonomical place
کاووس ایازپور Negahdar Oladhossein Ali PakniatBackground & Objectives: Tomato yellow leaf curl disease (TYLCD) is one of the most common and important tomato diseases in Iran. Sequences of viral genomes help to find and interbreed resistant plant varieties. This study aimed to sequence the com MoreBackground & Objectives: Tomato yellow leaf curl disease (TYLCD) is one of the most common and important tomato diseases in Iran. Sequences of viral genomes help to find and interbreed resistant plant varieties. This study aimed to sequence the complete genome of an isolate of the TYLCV, determine its strain and taxonomic status. Materials & Methods: For this purpose, a viral isolate from Assaluyeh was selected and studied. Using degenerate primers of begomoviruses (BC/PCRV 181) a fragment (500bp) of the genome was amplified in a polymerase chain reaction (PCR). According to the sequence of this fragment (500bp), a primer pair was designed (FarsC/FarsV) to achieve the full genome sequence of the virus. By utilizing a combination of primers (FarsC/PAL1V 1978) / (FarsV/TYLCV-[Ab] 1997C) complete sequence of Asalooyeh isolate was amplified and the taxonomic position of that isolate was determined. Results: Phylogenetic analysis showed that this isolate had a one-part genome and was more than 93% similar to the Omani strain. Therefore, this isolate is an Omani variant of the virus. In other researches, was stated that there is only TYLCV-IL strain in Bushehr province, while the results of this study prove the existence of TYLCV-OMN strain. Conclusion: According to the results of this study Asalooyeh isolate of TYLCV belongs to the Omani strain of TYLCV that produces severe yellowing in host plants. Manuscript profile -
Open Access Article
11 - Loop-mediated isothermal amplification: Rapid and cost-effective method for detection of pathogens
Ahzam Askari Mohammad Kargar Sadegh Ghorbani-Dalini Abbas DoostiLoop-mediated isothermal amplification (LAMP) is nucleic acid amplification method that amplifies target isothermally at 62-65˚C. In this approach, withouth thermal denaturation step, double stranded DNA is simultaneousely denatured and synthesized by using the Bst (Bac MoreLoop-mediated isothermal amplification (LAMP) is nucleic acid amplification method that amplifies target isothermally at 62-65˚C. In this approach, withouth thermal denaturation step, double stranded DNA is simultaneousely denatured and synthesized by using the Bst (Bacillus stearothrmophilus) DNA polymerase with high strand displacement activity. Thus the reaction can be conducted with a cost-effective quipment such as water bath or heating block, and the thermal-cycling needs of a PCR are avoided. The LAMP reaction uses six primers that specifically recognize eight distinct regions on the target sequence. Moreover, the LAMP reaction produces a large amount of amplified products, resulting in easier detection, such as visual judgment based on the turbidity or colour change result from fluorescent dsDNA intercalating dye in the reaction mixture, so gel electrophoresis is not required. Therefore, the LAMP assay has the advantages of high specificity, sensitivity, amplification efficiency and simple detection, that without the need for expensive equipment could be applicable as valuable tool for rapid diagnosis of infectious diseases in both clinical and hospital laboratories of developing countries. The aim of this article is to introduce the principles and applications of LAMP method for detection of infectious agents. Manuscript profile -
Open Access Article
12 - Discrimination and preliminary selection of self-compatible progenies among controlled crosses in almond using Sf specific primer
M. Shahmoradi M. Rasouli R. Footuhi Ghazvini A. Imani Y. Hamidogli -
Open Access Article
13 - Study of Compatibility Relationships Among Some Almond Cultivars and Genotypes Using of SAlleles Identification
M. Fallah M. Rasouli Y. Sharaf A. Imani -
Open Access Article
14 - Identification of Bacterial Isolates of Pseudomonas fluorescens siderophore from Rhizosphere of Corn Fields and Evaluation of Iron Absorption by Corn
Sayed Amin Fani Yazdi Amir Fotovat Amir Lakzian Ali Akbar Haddad Mashhadrizeh -
Open Access Article
15 - Molecular identification and detection of gene encoding deoxynivalenol in Fusarium graminearum isolates, the causal agent of fusarium head blight of wheat in Ira
Roya Rezaeian Doloei saeed rezaee mansooreh mieabolfathy hamidreza zamanizadeh mohammad razaviFusarium Head Blight (FHB) is one of the most important fungal diseases of wheat in Iran and throughout the world. Fusarium species infect wheat during the flowering period. In addition to losses of yield, these fungi can also synthesize mycotoxins under suitable enviro MoreFusarium Head Blight (FHB) is one of the most important fungal diseases of wheat in Iran and throughout the world. Fusarium species infect wheat during the flowering period. In addition to losses of yield, these fungi can also synthesize mycotoxins under suitable environmental conditions, thus threatening animal and human health. In the present study, 60 isolates of F. graminearum collected from infested wheat fields of some provinces of Iran and were characterized morphologically and genetically. Molecular characterization of isolates was performed using an optimized simple and low cost method for isolation of DNA from F. graminearum using polymerase chain reaction with species specific primers. All of the isolates were confirmed as F. graminearum using species specific primers Fg16F/Fg16R through PCR assays. The results indicated that a specific band of 420 bp was amplified, when species specific primers of F. graminearum was used. All of the isolates were molecularly identified as F. graminearum. The primers failed to amplify any band in negative controls including F. culmorum. All of F. graminearum isolates were tested for DON chemotype producing gene by specific Tri13F/Tri13DONR primer by PCR. Our results showed that a specific band of 282 bp was amplified only in 36 isolates (60%). Consequently, using of species specific primers for detection of suspected cultures of F. graminearum in infected tissues and toxin profile of isolates are very interesting when comparing with the morphological trait which are time consuming and non-specific. Manuscript profile
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