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      • Open Access Article

        1 - Application of Herbicide in Paddy Fields Inoculated with ‎Azotobacter chroococcum
        Akbar Shirzad Chenari Hashem Aminpanah Peyman Sharifi
        A field experiment was conducted at Rasht, in 2014, to investigate controlling weeds in native Hashemi cultivar paddy fields by different methods of herbicide applications and inoculated with Azotobacter chroococcum. The factorial experiment based on a randomized comple More
        A field experiment was conducted at Rasht, in 2014, to investigate controlling weeds in native Hashemi cultivar paddy fields by different methods of herbicide applications and inoculated with Azotobacter chroococcum. The factorial experiment based on a randomized complete block design with three replicates was performed. Factors were Azotobacter application with two levels (inoculation with or without Azotobacter chroococcum) and weed management regimes with six levels (pretilachlor plus a supplementary hand weeding, bensulfuron methyl plus a supplementary hand weeding, and pretilachlor + bensulfuron methyl plus a supplementary hand weeding, hand weeding during rice growing period + no herbicide, un-weeded during rice growing period + no herbicide). Results indicated that paddy rice yield was significantly increased by 16% after Azotobacter chroococcum inoculation. Rice paddy yield was significantly reduced in un-weeded plots compared to hand weeded and herbicide treated plots. ANOVA also revealed that there was no interaction effect between Azotobacter chroococcum applications and weed management regimes on paddy and biological yields, indicating that the herbicides had no adverse effect on Azotobacter efficiency in promoting growth and paddy yield of rice. Weed dry weight was reduced by 88, 91, 92, and 94 percentages in weeded plot and plots treated with pretilachlor, bensulfuron methyl, and pretilachlor + bensulfuron methyl, respectively, compared to un-weeded plots. Azotobacter chroococcum inoculation had no significant effect on weed biomass. Overall, the result of this experiment confirmed the feasibility of chemical weed control in paddy fields inoculated with Azotobacter chroococcum. Manuscript profile
      • Open Access Article

        2 - Effect of Azospirillum lipoferum Inoculation, Previous Crop, and Usage Nitrogen on Rice (Oryza sativa L.) Growth and Yield
        Milad Javadi Hashem Aminpanah
        Incorporation of winter legume crops in rotation with rice and using plant growth-promoting rhizobacteria can be a proper alternative approach in increasing sustainable crop production in rice fields. A split factorial field experiment using randomized complete block de More
        Incorporation of winter legume crops in rotation with rice and using plant growth-promoting rhizobacteria can be a proper alternative approach in increasing sustainable crop production in rice fields. A split factorial field experiment using randomized complete block design with three replications was conducted at the Rice Research Station of Tonekabon, Mazandaran province, Iran, in 2014 to evaluate the effects of previous crop, Azospirillum lipoferum inoculation, and N rate on growth and seed yield of rice (Oryza sativa L. cv. Shiroudi). Main plots were consisted of previous crop [berseem clover, faba bean, and control (fallow)] and subplots of Azospirillum lipoferum (Inoculated and Un-inoculated) and recommended rate of N applications (50, 75, and 100 kg.ha-1). Analysis of variance showed that rice paddy yield was significantly affected by previous crop, Azospirillum lipoferum and N rate. Result also showed that rice paddy yield was increased only by 3% when rice was planted after berseem clover as it compared with rice plant after fallow. However, rice paddy yield was significantly reduced by 16% when it was planted after faba bean as compared to that it was planted after fallow. Rice paddy yield was significantly increased by 14% after Azospirillum lipoferum inoculation. Rice paddy yield was significantly increased by 11% when N application increased from 50 to 75 kg N ha-1, and further N application (100 kg N ha-1) did not affect paddy yield significantly. Based on the result of this experiment, planting rice after berseem clover, Azospirillum lipoferum inoculation and application of kg N ha-1 of recommended rates can be used to obtain highest paddy yield in the experimental site. Manuscript profile
      • Open Access Article

        3 - Study on Changes of Atropine and Scopolamine Content and Growth Characteristics of Atropa belladonna L. affected by Bio-fertilizers and Chemical-fertilizers
        Mohammad Inanloo Far Hassanali Naghdi Badi Mostafa Heidari Majid Tolyat Abolhasani Hassan Makarian Mohammad Reza Amerian
          Abstract Nowadays, the use of Plant Growth Promoting Rhizobacter (PGPR) as a biofertilizers is one of the ways to improve growth and phytochemical characteristics of medicinal plants. This study was carried out as a factorial experiment based on randomized comple More
          Abstract Nowadays, the use of Plant Growth Promoting Rhizobacter (PGPR) as a biofertilizers is one of the ways to improve growth and phytochemical characteristics of medicinal plants. This study was carried out as a factorial experiment based on randomized complete block design with a randomized complete block design with 3 replications in the research farm of the Institute of Medicinal Plants - ACECR in 2017.The growth-promoting bacteria as a first factor were non-inoculation, Pseudomonas, Azotobacter, Pseudomonas + Azotobacter, and Thiobacillus + Sulfur. The chemical fertilizers as a second factor were no fertilizer or control, 50% recommended fertilizer and 100% recommended fertilizer. At flowering stage, the alkaloids were extracted by using chloroform, methanol and ammonia solvents. The amount of atropine and scopolamine was measured by High-performance liquid chromatography (HPLC). The biological and chemical fertilizers, as well as their interaction effect had significant effect (p<0.01) on growth traits, atropine and scopolamine content of leaf and root. The maximum biological yield was observed in Pseudomonas with 100% of recommended fertilizer. The highest amount of leaves atropine and scopolamine was related to Pseudomonas with 50% fertilizer and non-biofertilizer treatment with 50% recommended fertilizer, respectively. The highest amount of root atropine was related to Pseudomonas without chemical fertilizer application. The highest content of root scopolamine was observed in treatment of Pseudomonas with 100% recommended. Therefore, the highest biological yield and the amount of atropine in leaves and roots, as well as the scopolamine content of the root were obtained using Pseudomonas application. Manuscript profile
      • Open Access Article

        4 - Seed Priming With Plant Growth-Promoting Rhizobacteria Alleviate Salinity Stress In Wheat Plant
        Fateme Nasibi Effat Mousavi
      • Open Access Article

        5 - Effect of phosphorus rate, Rhizobium phaseoli inoculation and Nitragin biofertilizer on growth and yield of common bean (Phaseolus vulgaris L.)
        Meysam Ghanbarzadeh Hashem Aminpanah Hasan Akhgari
        A field experiment was conducted in Amlash, Guilan province to evaluate the effect of phosphorus rate (P), Rhizobium phaseoli inoculation and nitragin biofertilizer on growth and yield of common bean (Guilan landrace). The experiment was designed in a factorial arrangem More
        A field experiment was conducted in Amlash, Guilan province to evaluate the effect of phosphorus rate (P), Rhizobium phaseoli inoculation and nitragin biofertilizer on growth and yield of common bean (Guilan landrace). The experiment was designed in a factorial arrangement based on a Randomized Complete Block Design with three replicates. The factors were P rate, Rhizobium phaseoli application, and application of nitragin biofertilizer. Result showed that grain and fresh pod yields were significantly increased by 28% and 21%, respectively, as P application rate increased from 0 to 50 kg ha-1. No significant increase in grain and fresh pod yields were observed as P application rate increased from 50 to 75 kg ha-1, but further increase in P rate (75 kg ha-1) reduced grain and fresh pod yields significantly. Regression analysis showed that the highest grain (1403.9 kg ha-1) and pod (8510.7 kg ha-1) yields were obtained when phosphorus was applied at the rate of 65.4 and 59.3 kg ha-1, respectively. Grain and fresh pod yields were significantly increased by 7 and 10% with nitragin biofertilizer application, respectively. Moreover, grain and fresh pod yields increased by 17% and 20% after Rhizobium inoculation. Manuscript profile
      • Open Access Article

        6 - Maize- Peanut Intercropping Under Inoculated with Azotobacter chroococcum
        Siavash Pourjani Hashem Aminpanah mohammadnaghi safarzade vishekaei
        Intercropping and plant growth-promoting rhizobacteria improve sustainability of agro-ecosystems. To evaluate the effect of Azotobacter (Azotobacter chroococcum) inoculation on productivity of a peanut /maize intercropping system, a two-year study was design as a random More
        Intercropping and plant growth-promoting rhizobacteria improve sustainability of agro-ecosystems. To evaluate the effect of Azotobacter (Azotobacter chroococcum) inoculation on productivity of a peanut /maize intercropping system, a two-year study was design as a randomized complete block in a factorial arrangement with three replicates at Astaneh-ye Ashrafiyeh, Guilan province. Factors were Azotobacter inoculation [control (non-inoculated) and inoculated with Azotobacter chroococcum) and intercropping pattern (Sole cropping of peanut and maize, additive intercropping pattern at three levels (100% peanut + 100% maize, 100% peanut + 50% maize, and 50% peanut + 100% maize), and replacement intercropping pattern at three levels (50% peanut + 50% maize, 67% peanut +33% maize, and 33% peanut + 67% maize). Results showed that gain yields of maize and peanut were significantly affected by Azotobacter inoculation and intercropping pattern. Inoculation with Azotobacter chroococcum significantly increased grain yields of peanut and maize by 10% and 16%, respectively. Maximum and minimum of land equivalent ratio (LER) were observed at 100% peanut + 50% maize and 50% peanut + 50% maize, respectively and inoculated intercropped plots with Azotobacter chroococcum had 12-16% grater LER that non-inoculated ones. Regards to impossibility of mechanical weed control by machines in additive intercropping patterns, to obtain the highest productivity in maize/peanut intercropping system, intercropping pattern of 100% peanut + 50% maize along with Azotobacter application will be suitable in fields with low weed density. Otherwise, based on LER and grain yield of peanut, the intercropping pattern of 67% peanut + 33% maize along with Azotobacter application will be suitable. Manuscript profile