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Open Access Article
1 - The investigation of single nucleotide polymorphism rs2286663 in human Insulin-like 3 gene(INSL3),related to male infertility by RFLP PCR method
Asieh fotoohi رودابه بهزادی اندوهجردی مسعود زندیABSTRACT: Infertility is the inability of a couple to conceive after one year of consecutive sexual intercourses without birth control methods. Infertility is a multifactorial syndrome with several genetical or nongenetical reasons. The possible causes for infertility a MoreABSTRACT: Infertility is the inability of a couple to conceive after one year of consecutive sexual intercourses without birth control methods. Infertility is a multifactorial syndrome with several genetical or nongenetical reasons. The possible causes for infertility are still unknown for more than 25 percent of cases (idiopathic infertility). Recently a product of Leydig cells termed Leydig insulin –like hormone (INSL3)has been proposed as a putativa trophic hormone of the first part of descent.Absence of Insl3 in male mice results in bilateral cryptorchidism and mutations involving this gene may be a cause of azoospermia in man.this gene has several SNP .For this reason at this research rs2286663 is investigated. For this purpose, the number of 80 infertile men and 80 healthy men (as a control) was tested. Using a standardRFLP PCR method, the presence of the mutant was analyzed. The statistical results were not significant. Thus, no significant relationship between the mutation and idiopathic infertility in males has been detected. However, the results may be different by selecting the different genetic pool or significant changes in a population size. Manuscript profile -
Open Access Article
2 - The effect of a course of aerobic exercise with stem cells on some genes involved in mitochondrial biogenesis of azoospermic rats
Sahar Koochaki Hajar Abbaszadeh parvin farzanegiIntroduction: Spermatogenesis is highly dependent on energy metabolism and spermatogenesis is very sensitive to compounds that interfere with mitochondrial energy metabolism and cellular respiration control. The purpose of this research was to investigate the role of ex MoreIntroduction: Spermatogenesis is highly dependent on energy metabolism and spermatogenesis is very sensitive to compounds that interfere with mitochondrial energy metabolism and cellular respiration control. The purpose of this research was to investigate the role of exercise activity along with stem cells on the expression of some effective genes in mitochondrial biogenesis in azoospermic rats with busulfan.Methods: In this experimental study, 30 8-week-old rats were selected and randomly divided into 6 groups after induction of the azoospermia model. Healthy, sham, azoospermia, azoospermia+exercise, azoospermia+cell group, and azoospermia+cell+exercise group were divided. One month after the creation of the model, one million stem cells were transplanted once into the vas deferens of each mouse. Swimming training was done daily for 30 minutes a day and 5 days a week for 8 weeks. Genes were measured by Real time-PCR method. One-way analysis of variance was used to analyze the data. All calculations were done using SPSS/23 statistical software and at a significant level of P≤0.05.Findings: The results showed that the induction of the azoospermia model caused a decrease in the expression of SIRT1 and AMPK genes in testicular tissue, and that exercise combined with cell therapy increased the expression of SIRT1 and AMPK genes in testicular tissue in azoospermic model rats.Conclusion: In general, the results of the current research indicate that regular aerobic exercise such as low-intensity swimming helps in controlling the effects of infertility diseases through the maintenance and development of mitochondrial biogenesis in improving the spermatogenesis process. Manuscript profile -
Open Access Article
3 - Investigating of malondialdehyde levels in azoospermia model rats following a period of swimming exercises and stem cell transplantation
sahar kochaki Hajar Abbaszadeh parvin farzanegiThe purpose of this study was to investigate the malondialdehyde levels in azoospermia model rats following a period of swimming training and stem cell transplantation. Thirty 8-week-old rats were selected, and then the azoospermia model was created with the drug busulf MoreThe purpose of this study was to investigate the malondialdehyde levels in azoospermia model rats following a period of swimming training and stem cell transplantation. Thirty 8-week-old rats were selected, and then the azoospermia model was created with the drug busulfan with a dose of 40 mg. Rats were randomly divided into healthy control (n=5), sham control (n=5), azoospermia control(n=5), azoospermia+exercise (n=5), azoospermia+cell (n=5) and azoospermia+cell+exercise (n=5) groups. One month after the creation of the one-time model, stem cells were transplanted in the vas deferens in the amount of one million cells for each mouse, and after recovery, daily for 30 minutes in They swam 5 days a week for 8 weeks. Malondialdehyde levels were measured by thiobarbituric acid method and all calculations were done using SPSS/23 statistical software and at a significant level of p≤0.05. The results showed that induction of azoospermia model increased testicular tissue malondialdehyde levels (P=0.002) and exercise combined with cell therapy caused a non-significant decrease in testicular malondialdehyde levels in azoospermia model rats (P>0.05). In general, the results of the present research indicate an increase in malondialdehyde levels in testicular tissue cells of azoospermia model mice. On the other hand, regular aerobic exercise such as swimming along with cell therapy will probably help in controlling the effects of infertility diseases by reducing malondialdehyde levels and reducing oxidative stress. Manuscript profile -
Open Access Article
4 - The study of the association of two variants of MLH3(rs175080) andTEX11(rs6525433) in Iranian infertile men
Hanieh Sezavar Zahra Noormohammadi Masoud SheidaiInfertility is one of the most common problems in human societies which estimated between 1 in 6 couples worldwide. It is a complex clinical condition caused by the interaction of genetic and environmental factors. The TEX11 and MLH3 gene plays a key role in the mechani MoreInfertility is one of the most common problems in human societies which estimated between 1 in 6 couples worldwide. It is a complex clinical condition caused by the interaction of genetic and environmental factors. The TEX11 and MLH3 gene plays a key role in the mechanism of DNA repair and crossing over. Methods: In this study, the association of two SNPs of MLH3 (rs175080) and TEX11 (rs6525433) in Iranian infertile men was investigated. One hundred twenty infertile men with azoospermia and 115 healthy individuals were collected as a control. These two polymorphisms were investigated by using tetra-amplification refractory mutation system ((PCR), TETRA - ARMS – PCR), PCR – RFLP, and sequencing were used. Findings and Discussion: The frequency of GG and GA genotypes in MLH3 gene was 30.9% and 69.1% in azoospermia males and 31.3% and 68.7% in the control group (P = 0.619), respectively. The result showed that rs175080 polymorphism in the MLH3 gene and rs6525433 polymorphism in the TEX11 gene were not associated with the risk of Azoospermia. Manuscript profile -
Open Access Article
5 - The effect of low-power laser on the promotion of spermatogenesis in a mouse model of azoospermia (in-vivo)
habib tajalli Masoud Maleki Esmail Safavi Reza Shahi Fatemeh Firoozi Zahra Akbarpour Ali reza Sotoudeh Khyaban -
Open Access Article
6 - The effects of low-power laser on the promotion of spermatogenesis in a mouse model of azoospermia (in-vivo)
Habib Tajalli Masoud Maleki Esmail Safavi Reza Shahi Fatemeh Firoozi Zahra Akbarpour Alireza Sotoudeh Khyaban -
Open Access Article
7 - A review of common animal models in reproductive biology studies
ramazan khanbabaee nahideh nazdikbin yamchiOne of the major problems in today's world is the inability to have children. This has affected social health and led researchers to preclinical studies. Over several decades, numerous animal models in the biology of reproduction have entered the field of research. Diff MoreOne of the major problems in today's world is the inability to have children. This has affected social health and led researchers to preclinical studies. Over several decades, numerous animal models in the biology of reproduction have entered the field of research. Different mechanisms and approaches induce different types of reproductive diseases. Variety in conventional methods in inducing animal models has confused researchers. Since premature ovarian failure, azoospermia and polycystic ovary syndrome are important causes of inability of couples to have children, we decided to review the studies conducted in this field, appropriate laboratory methods and processes to create an animal model for premature failure. Introduce ovarian, azoospermia and polycystic ovary syndrome. The use of chemical drugs used in adults as chemotherapy drugs, especially cyclophosphamide, is known as the most appropriate method to create an animal model in premature ovarian failure. To create an animal model of azoospermia, busulfan injection is used as the most common method to induce azoospermia. Finally, to create an animal model of polycystic ovary syndrome, the most common method is to inject androgens into dihydroepiandestrone. In the present study, common methods along with the relevant protocols, along with the advantages and disadvantages of each model are presented. Manuscript profile -
Open Access Article
8 - The Effect of Stem Cell Factor and Retinoids in Spermatogonial Cell Differentiation of Testicular Tissue of Obstructive Azoospermia Model Adult Rats
Mahnaz Nasimi Esmaeil Fattahi Sayed Gholamali Jorsaraei Maryam Gholamitabar Tabari Ebrahim Zabihi NeyshabouriRetinoids and stem cell factor play an important role in the stem cells proliferation, survival, and differentiation into sperm. Therefore, in this study, the effect of stem cell factor and retinoids on the proliferation and differentiation of spermatogonial cells in te MoreRetinoids and stem cell factor play an important role in the stem cells proliferation, survival, and differentiation into sperm. Therefore, in this study, the effect of stem cell factor and retinoids on the proliferation and differentiation of spermatogonial cells in testis seminiferous tubules of obstructive azoospermia model adult rats was investigated using the tissue culture system. The testicular tissue fragments were cultured in the culture medium supplement with retinoids or stem cell factor, or a combination of both at the temperature of 34.5 °C and 5% CO2 for 25, 30, and 35 days. Then, the stained tissue sections prepared at the mentioned times were evaluated using PAS and hematoxylin staining methods. The cell counting per unit area was done using ImageJ software, and the statistical analysis was performed by one-way ANOVA and Tukey's post hoc test. The number of spermatogonia, spermatocyte, and Leydig cells increased significantly on day 25, 30 and 35 of culture; additionally, the number of round and elongated spermatid increased significantly on day 35 of culture in the testicular tissue of the obstructive azoospermia compared to the experimental and control groups (p < 0.001). Histological examination of testicular tissue in different culture conditions revealed pathological changes in the epithelialmaking cells. Retinoids played a more effective role on day 35 of culture compared to other factors in maintaining and proliferation of cell lines of the spermatogenesis process. As these factors are not alone the cause of proliferation and differentiation induction, therefore optimizing the culture medium is recommended to achieve adult sperm. Manuscript profile -
Open Access Article
9 - A study on histological changes of testis in mice treated with intratesticular gentamicin
وحید Akbarnejad پرویز Tajik M. Movahedin رضا Youssefi نیلوفر Haj SadeghiArrestment of spermatogenesis in the recipient is an important step in spermatogonial stem cell transplantation.Studies have shown toxic effects of gentamicin on proliferation and differentiation of bone marrow stem cells. Thepresent study was conducted to evaluate the MoreArrestment of spermatogenesis in the recipient is an important step in spermatogonial stem cell transplantation.Studies have shown toxic effects of gentamicin on proliferation and differentiation of bone marrow stem cells. Thepresent study was conducted to evaluate the effect of intratesticular administration of gentamicin on induction ofazospermia in mice. Twelve male mice were randomly assigned to three experimental groups: in control group,the mice received no treatment. In saline group, the mice received 0.02 ml saline intratesticularly. In gentamicingroup, the mice received 0.02 ml gentamicin (40 mg/ml) intratesticularly. Two months after treatment, the testeswere removed and their weight was recorded. Then, the specimens were fxed in 10% formalin solution, stainedand evaluated using light microscope. There was no difference among experimental groups in testicular weight andmortality (P> 0.05). Although germ cells were depleted in saline and gentamicin groups, spermatogenesis was notarrested in none of these groups based on histopathological examination. In conclusion, the results indicated thatintratesticular administration of gentamicin might not serve as an effective method for induction of azospermia inmice. Manuscript profile -
Open Access Article
10 - Clinical report of an Idiopathic Hypogonadotrophic Hypogonadism in a seven year old dog
اورنگ Ataee Amarluii, , S NaghibzadehOn a 7-years-old dog with Azoospermia three conventional drugs in breeding techniques were tested to assess the effects and reproductive improvement of them. the Drugs that chosen include gonadotropin (GnRH) in commercial name Cystorelin، 50 micrograms subcutaneously MoreOn a 7-years-old dog with Azoospermia three conventional drugs in breeding techniques were tested to assess the effects and reproductive improvement of them. the Drugs that chosen include gonadotropin (GnRH) in commercial name Cystorelin، 50 micrograms subcutaneously 60 minutes prior to sperm collection , prostaglandin (PGF2α) in commercial name Veteglan , 0/005 mg/kg subcutaneously 15 minutes prior to sperm collection and Oxytocin 10IU per dog intramuscularly 10 minutes prior to sperm collection. Semen collected from this animal by manual semen collection technique and we had 7 days sexual rest between each collection. during the semen collection the dog’s libido was assessed and then semen Volume , Semen PH , semen color , semen concentration per ml ,total count of spermatozoa , progressive motility forward percent , morphologically normal spermatozoa percent and Total normal motile spermatozoa (TMNS) was evaluated. The samples did not show any traces of semen quality change when dog received prostaglandin and oxytocin and there is no spermatozoa in that samples. But when the sampled animal was under the influence of gonadotropin therapy semen quality improved dramatically in addition we have one billion and twenty-four millions of spermatozoa as the total count of the sample. Two hypotheses are proposed for this quality improvement. The frst one say the prescribing of gonadotropin in this dog, increased the animal blood testosterone index and stimulate the seminiferous ducts to released spermatozoa. The second one say because of the clinical condition of the animal it seems to had IHH syndrome and during the administration of gonadotropin the body hormonal system was regulated and released the sperm cells. Finally, it can be concluding that the use of gonadotropins may be improving the quality of semen of animals that have Azoospermia with source of hormonal abnormality. Manuscript profile -
Open Access Article
11 - The effect of eight weeks of swimming training, cell and laser therapy on the expression of IL-2, IL-10 and STAT-3 genes in testicular tissue in azoospermia model rat.
seyed sasan Hoseyni Habib Asgharpour پروین فرزانگیpurpose: Azoospermia means lack of sperm in semen. One percent of all men and ten percent of infertile men suffer from azoospermia, the purpose of this study is to investigate the effects of eight weeks of swimming training, cell and laser therapy on the expression of I Morepurpose: Azoospermia means lack of sperm in semen. One percent of all men and ten percent of infertile men suffer from azoospermia, the purpose of this study is to investigate the effects of eight weeks of swimming training, cell and laser therapy on the expression of IL-2, IL-10 and STAT-3 genes in testicular tissue. It is azoospermia in rats. Methods: Forty 6- to 8-week-old rats were randomly selected, and then the azoospermia model was induced with Busulfan at a dose of 40 mg in mice. One month after induction of the model, stem cells were transplanted once in the vas deferens at the rate of one million cells per mouse. Then, one week after cell transplantation, a laser with a wavelength of 632.8 nm and a power of 10 mW and energy 3 joules were applied in three repetitions throughout the study period with an interval of once a week, and after improving the effects of surgery, swimming training were performed for 30 minutes and 5 days a week for 8 weeks. Results: The results showed that induction of azoospermia model caused a significant increase in IL-2, IL-10 and STAT-3 genes compared to healthy group rats (P≤0.05). Also, performing the interventional methods of laser therapy, cell therapy and exercise significantly reduced the expression of these genes in the testicular tissue of rats compared to the patient group (P≤0.05). Conclusion: Swimming exercise in combination with cell and laser therapy may help the fertility of azoospermia model rats by reducing testicular tissue inflammation signaling. Manuscript profile
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