Currently, global use of prebiotic compounds has increased considerably. This study aims to analyse the effect of complementing the rations of rainbow trout with different concentrations of Celmanax® prebiotic, which contains Saccharomyces cerevisia associated More
Currently, global use of prebiotic compounds has increased considerably. This study aims to analyse the effect of complementing the rations of rainbow trout with different concentrations of Celmanax® prebiotic, which contains Saccharomyces cerevisia associated compounds with Mannan-oligosaccharide on gill and liver histology during the growing period and experimental challenge with yersiniosis. Four concentration levels of prebiotic (0, 0.1, 0.5 and 1%) were mixed into fish feed pellets. The fish (with mean body weight of 19.08±1.45 gr) were fed a supplemented commercial diet for 60-days.On day 60 of the study, experimental yersiniosis was induced in all treatment groups by intraperitoneal injection of bacterial suspension. Tissue samples were taken on days 0, 30 and 60 and also after induction of experimental yersiniosis. The results showed that complementing rainbow trout rations with different concentrations of Celmanax® improved the gill and liver injuries on days 30 and 60 of the study. The best tissue effects in the growing period were observed in treatment groups which received 0.1% and 0.5% of the prebiotic respectively, and after experimental challenge with yersiniosis, the lowest lesions of liver and gill pathology were seen in the treatment group fed with 0.1% prebiotic which was significantly different (p<0.05) from all other treatments. According to the results of this study, adding Celmanax® to rainbow trout diet at 0.1% concentration improves tissue parameters during breeding period and decreases tissue lesions when faced with yersiniosis. Conflict of interest: None declared.
Manuscript profile
Streptococcosis and Yersiniosis are important diseases for the aquaculture industry for the rainbow trout (Oncorhynchus mykiss). In this study, the specific immunological responses of rainbow trout to vaccination with the bivalent vaccine of Streptococcus/ Ye More
Streptococcosis and Yersiniosis are important diseases for the aquaculture industry for the rainbow trout (Oncorhynchus mykiss). In this study, the specific immunological responses of rainbow trout to vaccination with the bivalent vaccine of Streptococcus/ Yersinios were investigated. Three hundred rainbow trout fingerlings (25±2g) were randomly divided into four equal groups in triplicates. Treatments contain Streptococcosis vaccine, Yersiniosis vaccine, bivalent vaccine, and control group. Fish in vaccinated treatment intraperitoneally injected with 100 microliter vaccine on the first day. The control group was injected with sterile PBS, identical to the vaccinated group. Blood samples were collected from vaccinated and non-vaccinated groups at days 0, 30, and 60 of the study, and antibody titer was subsequently performed. Sixty days after initial immunization, all groups were challenged by a lethal dose of Streptococcus iniae and Yersinia rockeri, mortality rate was calculated and compared among the groups for 14 days. These results indicated high antibody levels in the vaccinated fish at day 30 and 60 post-immunization using both vaccines(p<0.05). However, this amount was fewer at day 60 than day 30. The percentage of trout cumulative mortality after the challenge with Streptococcus iniae and Yersinia rockeri showed a significant decrease in vaccinated groups compared to the control treatment. In general, it can be concluded that the administration of bivalent Streptococosis/Yersiniosis vaccine to the rainbow trout provided appropriate protection against disease and effectively induced the immune response against these two diseases, which is comparable to either of the vaccines separately.
Manuscript profile
Yersiniosis or Enteric Red Mouth Disease (ERM) is an infectious bacterial disease that causes mortality and alsoeconomical loss in Salmonid aquaculture industries. In recent years, the yersiniosis has been expended in differentpart of Iran and there are reports about it More
Yersiniosis or Enteric Red Mouth Disease (ERM) is an infectious bacterial disease that causes mortality and alsoeconomical loss in Salmonid aquaculture industries. In recent years, the yersiniosis has been expended in differentpart of Iran and there are reports about it in most of the Rainbow trout farms in Iran. Mazandaran Province hasthe second grade of Rainbow trout production in Iran and the most production of Mazandaran is in Haraz Zone(East of Mazandaran). There for, because of the importance of Yersiniosis and also high production of Rainbowtrout in this part of Mazandaran, factors influencing on yersiniosis incidence was evaluated. Samplings were donefrom July 2011 to August 2012 and in 10 rainbow trout farms. Overall, liverand kidney of 1200 fsh with clinicalsigns (410 samples) either without clinical signs (790 samples) were Cultured in TSA agar (Merck Co. Germany).Then Microbial cultures were transformed to laboratory (Ecology of Caspian Sea Institute, Health and Aquaticanimal’s Disease branch) and Bacteria were identifed using gram staining and biochemical tests. In addition, thewater of farms was sampled for calculating Bacterial total count and also measuring some of the physicochemicalparameters (Temperature, DO, pH, Nitrate, Nitrite, NH4+ and TDS) of water. Result showed that decrease oftemperature and increase of pH had the most effects on incidence of yersiniosis . Also, it was demonstrated thatfrom 410 diseased fsh and 790 fsh without clinical signs, 20% and 10% suffered from yersiniosis respectively.
Manuscript profile
Sanad
Sanad is a platform for managing Azad University publications
