List of Articles Walnut Anthracnose

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  1 - Growth and sporulation of some Gnomonia leptostyla isolates in various culture media
  Soleiman Jamshidi Siyamak Salahi
  Walnut anthracnose is the most common disease in common walnut, Juglans regia L., in Iran, caused by Gnomonia leptostyla. In current research, efficiency of various culture media including BAB, OMA, MA, NA, PDA, CMA, WLEA and WLEOMA in growth and sporulation of the fung More

  Walnut anthracnose is the most common disease in common walnut, Juglans regia L., in Iran, caused by Gnomonia leptostyla. In current research, efficiency of various culture media including BAB, OMA, MA, NA, PDA, CMA, WLEA and WLEOMA in growth and sporulation of the fungus were studied. Three contaminated leaf samples from Miyaneh (No1), Marand (DV) and Karaj (Md1) areas were selected and after fungus isolation, they were cultured on mentioned media with three replications. Cultures were incubated in 21oC, 50% RH and 12:12 (L:D) photoperiod and some traits such as maximal colony diameter, colony pattern, mycelium density, sexual and asexual reproduction were investigated after 2, 3, 6, 9, 14 and 21 days. For perithecia maturation, primordial perithecia consisted cultures were incubated in 4oC in darkness for 3 months. There was no significant difference between isolates in morphology, color and growth pattern, but in growth rates and sporulation. Md1 produced primordial perithecia on the culture without walnut leaf extract but the others were not able to do. No1 could not sporulate on PDA, WA, CMA and OMA media as much as other isolates. However all isolates could produce immature perithecia and acervuli on WLEA and WLEOMA. The weakest media was BAB and the most suitable media for fungus isolation was WA. Fungus growth rate was in maximum on OMA and the highest density of mycelium was obtained on PDA. Sexual and asexual reproduction has been occurred in best way on media with walnut leaf extract. However, any mature perithecia was not obtained in any culture after three month incubation.  Manuscript profile
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  2 - Investigation on the genetic structure of Gnomonia leptostyla populations by PCR-RFLP in Azarbayejan-e-Sharqi, Iran
  Siyamak Salahi Mohammad Javan Nikkhah Soleiman Jamshidi
      Gnomonia leptostyla (Fr.) Ces. et de Not., the teleomorph of Marssonina juglandis (Lib.) Magn., is the causal agent of the walnut anthracnose, a wide spread disease in almost all the walnut growing areas and causes severe damages particularly in the n More

      Gnomonia leptostyla (Fr.) Ces. et de Not., the teleomorph of Marssonina juglandis (Lib.) Magn., is the causal agent of the walnut anthracnose, a wide spread disease in almost all the walnut growing areas and causes severe damages particularly in the north of iran such as Kermanshah, Azarbayejan, Khorasan and Karaj regions. Sampling was conducted from several areas of Azarbayejan-e-Sharqi province during 2005-2006. 60 Fungus isolates were isolated from samples and cultured as streaked single spore on OMA. Molecular techniques based on PCR-RFLP applied to investigate the genetic variability of G. leptostyla.Total DNA was isolated from mycelial powder by rapid mini-preparation method. A region of 60 isolates coding for the small-subunit ribosomal RNA (SrDNA-18s) and the internal transcribed spacer (ITS) were amplified and analyzed by restriction enzyme digestion. The results of PCR-RFLP showed no polymorphism either in length or in pattern among all the isolates tested. Manuscript profile