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        1 - The effect of different energy levels of diet on blood parameters and testicular tissue in sheep
        Maryam Feli Saied Mohammadzadeh Mohsen Abasi
        Purpose: One of the most important costs of sheep breeding is feed and the concentrated part of the ration. This research was conducted in order to investigate the effect of different rates of concentrate and fodder in lambs. Materials and methods: 27 Lori Bakhtiari ma More
        Purpose: One of the most important costs of sheep breeding is feed and the concentrated part of the ration. This research was conducted in order to investigate the effect of different rates of concentrate and fodder in lambs. Materials and methods: 27 Lori Bakhtiari male lambs with an average age of 75 days and a weight of 26kg were selected and divided into three treatments and nine replications. Treatment one was 55:45 concentrate to fodder, second treatment was 70:30 and third treatment was 15:85 and feeding was done individually in morning and evening. After the end of the 100-day period, blood sampling was done and all the lambs were slaughtered. The testicles were removed from the scrotum and after measuring the morphometric parameters, the testicular tissue sample with dimensions of 1x1cm was prepared in 10% formalin solution and tissue sections were prepared. Findings: The results showed that the rate of concentrate and fodder made a significant difference in the macroscopic parameters of testicular tissue (P˂0.05). The weight of the testis in the treatment of three lambs decreased. In this treatment, the thickness of the coating of spermatogenic tubes, tube diameter, lumen diameter and tube area decreased. Also, the rate of hematocrit, red blood cells and hemoglobin of this treatment was significantly higher than other treatments (P˂0.05). Conclusion: increasing the percentage of concentrate more than 70% will have a negative effect. Manuscript profile
      • Open Access Article

        2 - Investigation of the effects of copper sulfate on sperm quality parameters, DNA fragmentation rate and testicular tissue of adult rats of wistar breed
        rahil jannatifar hamid piroozmanesh Leila naserpoor
        Aim: Copper sulfate is one of the most important environmental pollutants that has the ability to produce free radicals and create oxidative stress. The aim of this study was to investigate the effects of copper sulfate on sperm quality parameters, DNA fragmentation rat More
        Aim: Copper sulfate is one of the most important environmental pollutants that has the ability to produce free radicals and create oxidative stress. The aim of this study was to investigate the effects of copper sulfate on sperm quality parameters, DNA fragmentation rate and testicular tissue of adult Wistar rats. Materials and Methods: In this experimental study, 30 adult Wistar rats weighing 250-200 grams were used. Random adult mice were treated in 3 control groups, copper sulfate receptor with concentration (100 mg / kg) and concentration (200 mg / kg) for 56 days. At the end of the treatment period, testicular weight, sperm count and parameters were assessed based on (WHO2010). The quality of sperm chromatin was assessed by acridine uranium nuclear pigments. Malondialdehyde level was measured. Data were analyzed using the One-Way ANOVA statistical method. Results: The quality of sperm parameters in copper sulfate with a concentration of 200 mg / kg decreased significantly (P <0.05). The testicular weight was significantly reduced at a dose of 200 mg / kg (P <0.05). The diameter of the seminiferous tubules, testosterone levels,, germ cell count, and sperm DNA fragmentation rate decreased at a dose of 200 mg / kg (P <0.05). The concentration of malondialdehyde at a dose of 200 mg / kg sulfate copper was significantly increased (P <0.05). Conclusion: This study shows that high concentrations of copper sulfate cause destructive effects on sperm quality, testicular tissue. Manuscript profile
      • Open Access Article

        3 - Effect of Sublethal Concentrations of Diazinon in Testicular Tissue in Zebrafish (Danio rerio)
        معصومه درویشی مجره رقیه صفری
        Inroduction & Objective: Diazinon is an organophosphoric pesticide used in agricultural fields. The aim of this study was to evaluate the effect of sublethal concentrations of diazinon on testicular tissue in Danio rerio.  Material and Methods:For this purpose More
        Inroduction & Objective: Diazinon is an organophosphoric pesticide used in agricultural fields. The aim of this study was to evaluate the effect of sublethal concentrations of diazinon on testicular tissue in Danio rerio.  Material and Methods:For this purpose, 240 male animals with mean weight of 0.20 ± 0.05 were subjected to 0.8, 1.6 and 3.2 mg / l of diazinon and control for 30 days. Results: In histopathological study, the highest abnormality was observed in the highest dose (3.2 mg / l) and the most important abnormality was reduction in gonad size. In control group, the testicular tissue had adequate size and growth, and the cells were in the sexual maturity stage, but in 0.8 mg/ l, cellular atrophy and lose of lobular walls was observedIn 1.6 mg / l, the degeneration and atrophy of the sexual cells, destruction of shape and walls of the lobules was observed. At the highest concentration, the degeneration and atrophy of the sexual cells, destruction of shape and wall of the lobules and the decrease in the number of sex cells were observed. This decrease in the number of sexual cells results in reduction of testicular tissue size. Conclusion: These observations indicate an increase in complications and anomalies with the increase in toxin concentrations Manuscript profile
      • Open Access Article

        4 - Effects of alcoholic extract of Euphorbia cyparissias on spermatogenesis and serum level of testosterone in streptozotocin-induced diabetic rats
        نعیمه Mehanfar M.A Nourazar مهدی Rahnema
        Diabetes mellitus is an endocrine disorder which is considered one of the main causes of mortality in developed countries. Long term use of the majority of synthetic anti-diabetic drugs lead to various side effects therefore the use of medicinal plants has currently bec More
        Diabetes mellitus is an endocrine disorder which is considered one of the main causes of mortality in developed countries. Long term use of the majority of synthetic anti-diabetic drugs lead to various side effects therefore the use of medicinal plants has currently become popular. Traditionally, Euphorbia cyparissias has been used for its anti-inflammatory, anti-oxidative and anti-neoplastic properties. In the present study 40 male Wistar rats were studied in four groups (n=10). The groups included: control, diabetic, alcoholic 500, alcoholic 250. The alcoholic groups received 250 and 500 mg/kg of alcoholic extract of Euphorbia cyparissias for 21 days through gastric gavage following induction of diabetes with streptozotocin. In control and diabetes induced groups an identical volume of physiological serum was gavaged. The testicular tissue and blood samples were obtained at the end of the experimental period for histological and blood testosterone concentration purposes, respectively. Results suggested that the serum glucose and testosterone concentrations in the control and alcoholic 500 groups had a significant difference with the other groups (p Manuscript profile
      • Open Access Article

        5 - Effect of Testosteroneand PMSG Hormones on Organ Culture of Immature Testis of NMRI Mice
        ندا غلامی نسیم حیاتی رودباری کاظم پریور ویدا حجتی
        Spermatogenesis that including complicated and precise process of cell differentiation in mammals ,begins in early adulthood and continues throughout the life of reproduction , which resulted in dividing of  fundamental cells , and result of dividing meiosis ,are haploi More
        Spermatogenesis that including complicated and precise process of cell differentiation in mammals ,begins in early adulthood and continues throughout the life of reproduction , which resulted in dividing of  fundamental cells , and result of dividing meiosis ,are haploid spermatids that basically have been chenged in their testicular and epididym because of creating high performance sperm.All of stages of spermatogenesis occur simultaneously, as in pathological conditions, the smallest disruption can lead to infertility.Beginning in terms of survival and spermatogenesis by FSH and testosterone hormones are regulated. Some researchers believe that the testosteronehormone is the main hormone for regulating of spermiogenesis and FSH has a minor role. PMSG has the similar performance to FSH. in this project we will consider the effects of sex hormones PMSG and testosterone on testicular tissue and spermatogenesis. IN VITRO experiments was done in three different groups including a controlt and two experimental groups on the immature male mice that were NMRI descent and duration of tissue culture was considered 4weeks.after passing specified time and doing histology and microscopy studying, Results: the results indicated meaningful increase in number of spermatosist, spermatid, sperm and decrease in A spermatogonia Certoli cells. Conclusion:These results indicate that testicular tissue culture can be a useful experimental model for studying the regulatory mechanisms of proliferation of spermatogonia. Manuscript profile