List of Articles Proteus

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  1 - Chemical Composition of the Essential Oil of Mentha pulegium L. and its Antimicrobial Activity on Proteus mirabilis, Bacillus subtilis and Zygosaccharomyces rouxii
  M.A. Khosravi Zanjani N. Mohammadi M. Zojaji H. Bakhoda
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  2 - Isolation and Identification of Cadmium Resistant Proteus sp. and Its Potential in Cadmium Bio Removal
  Marjan Mirhosseininia Maryam Ghane Parisa Nejatkhah
  10.22034/jest.2018.18266.2709
  Background: The contamination of ecosystems with heavy metals is an important issue in current world. Removal of heavy metals from contaminated sites using microorganisms is a cheaper alternative to chemical technologies. The aim of present study was isolation and chara More

  Background: The contamination of ecosystems with heavy metals is an important issue in current world. Removal of heavy metals from contaminated sites using microorganisms is a cheaper alternative to chemical technologies. The aim of present study was isolation and characterization of Cadmium resistant bacteria, determination of Minimum Inhibitory Concentration (MIC) and bio removal potential of the isolates. Materials and Methods: Isolation of Cadmium resistant bacteria was carried out by enrichment method by medium supplemented with Cadmium chloride. The minimum inhibitory concentration of Cd2+ was determined by the agar plate dilution method and the Cadmium removal evaluated by atomic absorption spectroscopy. Identification was carried out in accordance with Bergey's Manual of Systematic Bacteriology and phylogenetic analysis was performed based on 16S rRNA gene sequences. Results: A total of 40 Cadmium resistant strains were isolated from Salour River in Islamshahr. The results showed that bacterial strain ST1 isolated from sediments was highly resistant to Cadmium. The MIC of Cd2+ for selected isolate was 6 mM. The isolate was able to remove 65.2% of Cadmium at Cadmium concentration of 1 mM. Phylogenetic analysis showed that ST1 belongs to the genus Proteus with 99% similarity to Proteus mirabilis then designated as Proteus sp. HM_AF12. The strain had a wide pH tolerance of 5.5–9.0, and salt tolerance was up to 7.5% NaCl. Conclusion: The results implied that Proteus sp. HM_AF12 can be a low cost and environmental friendly bio sorbent that may have important application in Cd2+removal from polluted environment. Manuscript profile
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  3 - Evaluation of Immunogenicity of PLGA - Proteus  mirabilis Detoxified Lipopolysaccharide Conjugate Against Urinary Tract Infection in Mice
  Reza Shapouri
  Proteus is a gram negative and  facultative anaerobic opportunistic pathogen. This bacterium is responsible for 10% of urinary tract infections. A cause of 90% of proteus-induced infection is the mirabilis. Increasing the multi-drug resistance and the side effects More

  Proteus is a gram negative and  facultative anaerobic opportunistic pathogen. This bacterium is responsible for 10% of urinary tract infections. A cause of 90% of proteus-induced infection is the mirabilis. Increasing the multi-drug resistance and the side effects of antibiotics have led to a special concern for this purpose, finding preventive strategies like making a vaccine seems essential. In the present study, LPS from Proteus mirabilis, was extracted, detoxified and conjugated to PLGA nanoparticle. This conjugation was confirmed with ZetaSizer, FTIR and AFM. Vaccination was done intramuscularly, 3 times in two weeks intervals. Then, mices were anesthetized and operated with ethical rights and 6×107 CFU concentrations of P.mirabilis was injected into their bladder. After one week of injection, bladders were removed and suspended in a salt buffer.The results of the challenge test confirmed the success of the PLGA-LPS conjugate in comparison with other groups. In other words, the control and PLGA groups had the highest growth rates, and the average number of colonies was significantly higher than all of the groups. And the number of colonies grown in groups were LPS-PLGA(6.2 *10 cfu/ml) , LPS(3.6 ×103 cfu/ml), PLGA (91.3×102 cfu/ml) and NS(98 × 102 cfu/ml). According to one-way ANOVA, which was done by SPSS software, there was a significant difference between all groups (P <0.05).the PLGA immunogenicity was significantly different with the control group. Therefore, it can be concluded that the PLGA-LPS conjugate has an appropriate immunity and can be a candidate of nano-vaccine for urinary tract infection of P.migrailitis. Manuscript profile
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  4 - Simulation and Analysis of the New Brake System according to Changing of the Spring and Vertical Acceleration of the Wheels
  Bahman Karami Pirmohammadi Mehdi Tajdari
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  5 - Evaluation of the effect of Myristic acid and iron nanoparticles on the expression of Proteus Mirabilis pathogenic genes
  Zeinab Ashenagar kumarss amini
  10.30495/jmw.2021.690454
  Background: Proteus is one of the most important causes of UTI and nosocomial infections. Nowadays, the focus is on the antibacterial effects of natural products. The aim of this study was to investigate the effect of myristic acid and iron nanoparticles on the expressi More

  Background: Proteus is one of the most important causes of UTI and nosocomial infections. Nowadays, the focus is on the antibacterial effects of natural products. The aim of this study was to investigate the effect of myristic acid and iron nanoparticles on the expression of Proteus Mirabilis pathogenic genes isolated from urine samples.Materials and Methods: In this cross-sectional study, urine samples were collected from outpatients and patients admitted to hospitals and clinics during six months. After identification of Proteus mirabilis using standard methods, these isolates were tested for antibiotic resistance. After identification of the target gene bacteria, iron nanoparticles and myristic acid were treated and the expression of flaA and ureA genes in these bacteria were evaluated.Results: Finally, 6219 positive urine samples were collected from which 3937 (63.3%) were female and 2282 (36.7%) were male (mean age 16 69 69 years). Sixty Proteus mirabilis strains were isolated. Out of 60 clinical samples, flaA gene was present in 100% and UreA gene in 91.65% of isolated bacteria. The results showed that the expression level of flaA gene in isolates treated with mixture of myristic acid and iron nanoparticles decreased by one quarter of normal in isolates (p = 0.002).Conclusions: The data showed that iron oxide and meristic acid nanoparticles have strong antibacterial activity against Proteus mirabilis strains with flaA gene. In addition, these compounds decreased flagellin gene expression, which may indicate regulatory systems responsive to external signals. Therefore, these two substances can be considered as grounds for further studies on infection control. Manuscript profile