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        1 - Comparison of morphological and phytochemical traits in some endogenous genotypes of sweet violet (Viola odorata l.) in Mazandaran and Golestan provinces
        Hossein Moradi Mahdi Haddadinejad Alireza Yavari Mahdi Mohammadi Azni SeyedMaryam Musavi Syyed Mohammad Amin Hosseini
        In order to evaluate genetic diversity using phenotype and phytochemical properties of sweet violet (Viola odorata), 10 regions of Mazandaran and Golestan provinces were studied. Phytochemical traits such as phenol content, flavonoid, antioxidant capacity, chlorophyll a More
        In order to evaluate genetic diversity using phenotype and phytochemical properties of sweet violet (Viola odorata), 10 regions of Mazandaran and Golestan provinces were studied. Phytochemical traits such as phenol content, flavonoid, antioxidant capacity, chlorophyll a, chlorophyll b, total chlorophyll, and morphological characters of leaf size, petiole length, pedicel length, and root length were evaluated. Sari and Kosout genotypes had the highest chlorophyll b and total chlorophyll contents and the Sari ecotype had the highest flavonoid content. The highest level of antioxidant capacity was observed in Sari and Vari ecotypes. The ecotypes of Zanglab, Aq Cheshmeh, Tangeh Chehel Chai with lower height showed the highest total phenol contents. Results of morphological evaluation showed that ecotypes of Vari, Sar,i and kosout had the longest leaves and  ecotypes of Azni and Vari had the longest roots. Also, ecotypes of Vari, kosout, Tangeh Chehel Chai, and Zanglab had the highest petiole length and Tangeh Chehel Chai ecotype had the highest leaf number. Correlation of traits showed that leaf height, leaf width, petiole length, leaf number, leaf flavonoid, and leaf phenol contents of sweet violet decreased with increasing altitude. In general, Sari, Vari, and Kosoot ecotypes from Mazandaran province had superior genotypes for most of the traits that could be used for future breeding programs. Manuscript profile
      • Open Access Article

        2 - Variation on biological activities and total phenol and flavonoids contents in different extracts from Salvia rhytidea Bent. collected from natural habitats of Kerman and Sistan and Baluchestan provinces
        Omid Azizian-Shermeh Alireza Hassanabadi
        Introduction: The variety of quantity and quality of active ingredients as well as the antioxidant function of medicinal plants under different ecological conditions has made plants as a valuable and natural source of new antioxidants in the focus of researchers. Salvia More
        Introduction: The variety of quantity and quality of active ingredients as well as the antioxidant function of medicinal plants under different ecological conditions has made plants as a valuable and natural source of new antioxidants in the focus of researchers. Salvia rhytidea Bent is one of the native medicinal plants of Sistan and Baluchestan and Kerman provinces. The genus Salvia belongs to the genus Lamiaceae, with more than 311 species worldwide, especially in tropical and temperate regions. The present study is carried out to evaluate the phytochemical compounds such as total phenolic and total flavonoid contents, antioxidant and antibacterial activities of three extracts (Methanolic, Ethanolic and Aqueous) of Salvia rhytidea Bent from natural habitats of Kerman and Sistan and Baluchestan province.Experimental:  Phenolic and Flavonoid contents were determined by Folin-Ciocaltiu, Aluminum Chloride colorimetric and antioxidant activities were studied by two methods (DPPH and FRAP) and antimicrobial activities were estimated by Disk-Diffusion Agar and MIC methods against 3 bacteria Staphylococcus aureus, Bacillus cereus, Escherichia coli and 2 fungis Aspergillus niger and Candida albicans.Results and Disscussion: The results showed that, the methanolic extract of S. rhytidea from Sistan and Baluchestan province had high amount of secondary compounds (phenolic and flavonoids: 35.12 ± 2.11 mg GAE/g extract, 29.13 ± 1.12 mg QUE/g extract) and activities (antioxidant activities: IC50= 27.28 ± 2.12 µg/ml, 49.17 ± 2.21 mM Fe2+/mg Sample, and antimicrobial activities against Staphylococcus aureus with the diameter of inhibition zone 25 ± 1.41). Aqueous extract of S. rhytidea from Kerman province had minimum amount of secondary compounds (phenolic and flavonoids: 10.72 ±1.21 mgGAE/g extract, 6.14 ± 0.24 mgQUE/g extract) and activities (antioxidant activities: IC50= 173.12 ± 4.16 µg/ml, 7.66 ± 0.23 mM Fe2+/mg Sample, and antimicrobial activities against Escherichia coli with the diameter of inhibition zone 9 ± 0.19 mm).  The extraction method of the extract and the appropriate solvent play an important role in the extraction of secondary compounds. Methanol solvent has played an important role in the extraction of phenolic and flavonoid secondary compounds, and these compounds have led to antioxidant and antimicrobial properties, so that methanolic extracts have the highest amount of total phenols and flavonoids and the most antioxidant effect. Among the studied extracts, methanolic extract showed the greatest effect in all evaluations. Also, the antioxidant power of methanolic extract of this plant was so high that it was almost close to the antioxidant power of synthetic antioxidants ascorbic acid (10.75 ± 0.98 µg/ml, 65.75 ± 3.35 mM Fe 2+/mg Sample) and BHT (15.26 ± 1.2 µg/ml, 57.96 ± 1.53 mM Fe 2+/mg Sample) in both methods. Ethanolic extract was in the second place for both plant samples and between methanolic and aqueous extracts.Extension:  Overall, based on the results, the Salvia rhytidea can be a suitable candidate for the treatment of diseases caused by oxidative stress, and diseases caused by pathogenic microbes. Also, due to its high potential in eliminating pathogens, this plant can be a suitable alternative to antibiotics. Manuscript profile