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        1 - Phylogenetic groups of Escherichia coli strains isolated from urinary tract infection in Jahrom city, southern Iran
        Sara Asadi kavous Solhjoo Mohammad Kargar Abbas Ali Rezaeian
        Background and Objective: Escherichia coli is a causative agent of over 80% of urinary tract infections in all ages of the society. Strains of Escherichia coli are divided into four phylogenetic groups. Most of pathogenic extra-intestinal strains often belong to groups More
        Background and Objective: Escherichia coli is a causative agent of over 80% of urinary tract infections in all ages of the society. Strains of Escherichia coli are divided into four phylogenetic groups. Most of pathogenic extra-intestinal strains often belong to groups D and B2. This study aimed to define the phylogenetic groups of E. coli isolated from urinary tract infections in south of Iran. Material and Methods: This cross– sectional study was carried out on the patients with urinary tract infection who admitted to peymanieh hospital of Jahrom in 2010. Specific biochemical tests were used for identification of bacteria. The phylogenetic groups of E. coli strains were determined by the PCR method and using two specific primers yjaA ChuA and TspE4.C2. Results: Out of 60 identified E. coli, 78.34% were isolated from women while just 21.76% were isolated in men. The most common identified groups were classified as D (70%), A (23.3%) and B1 (6.7%), and none of the species belonged to the B2 group.  Data analysis revealed no significant relationship between phylogentic groups with the variables age, sex, history of urinary tract infection, previous history of antibiotic use and hospitalization. Conclusion: The results showed that most of the E. coli strains isolated from urinary tract infections in this region belonged to phylogenetic group D. Also, the results obtained from this region was different from other area. Manuscript profile
      • Open Access Article

        2 - Survey infection of Genetic diversity of E. coli strains isolated from beef cattle in sanandaj
        Hiva karimi darhabi
        Escherichia coli often lead to foodborne pathogen and generally considered public health importance. Escherichia coli can be produce one or more Shiga toxins, which may produce diarrhea, hemorrhagic colitis and life-threatening hemolytic uremic syndromein humans and ani More
        Escherichia coli often lead to foodborne pathogen and generally considered public health importance. Escherichia coli can be produce one or more Shiga toxins, which may produce diarrhea, hemorrhagic colitis and life-threatening hemolytic uremic syndromein humans and animals .The aim of the present work was to investigate the Escherichia coli groups A, B1, B2 and D of 80 samples from beef cattle in sanandaj . result shows that 34 samples(42.5%) E. coli isolated and identified based on standard procedures and PCR method with uidA gene .phylogenetic group of each strain was determined by using multiplex PCR method with chuA ,yjaA and segment of DNA TspE4C2. In this study, Among the strains isolated e.coli from beef cattle were allocated into phylogenetic group A (61.76%), B2 (5.8%), D(26.62%) and group B 1 (8.82%). Detection of E.coli isolation is very important and shows that food of animal origin can be of a reservoir that potentially could be transferred to humans the food chain. Manuscript profile